Mammalian Spermatogenesis, DNA Repair, Poly(ADP-ribose) Turnover: the State of the Art

Faraone-Mennella, Maria Rosaria

doi:10.5772/23337

Cited by 6 publications

(2 citation statements)

References 102 publications

(106 reference statements)

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“…Infact, the enzyme acts as a molecular DNA nick sensor binding DNA strand breaks with high affinity [5]. This interaction stimulates PARP-1 catalytic activity and is the first step in a series of events leading to either DNA repair or apoptosis (reviewed in [5,6,7]).…”

Section: Introductionmentioning

confidence: 99%

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

Imperato

Mistretta

Marone

et al. 2019

Cells

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Increased DNA damage and the propension to cancer development, depend on the modulation of the mechanisms to control and maintain genomic integrity. Poly(ADP-Ribose)Polymerase activation and automodification are early responses to genotoxic stress. Upon binding to DNA strand breaks, the enzyme, a molecular DNA nick sensor, is hyperactivated: this is the first step in a series of events leading to either DNA repair or apoptosis. Enzyme hyperactivation and automodification can be easily measured and are widely used to look at DNA damage extent in the cell. We investigated whether these two markers (increased catalytic activity and auto modification), could help to monitor DNA damage in lymphocytes of flower growers from Southern Italy, occupationally exposed to pesticides. Peripheral lymphocyte lysates were analyzed for Poly(ADP-Ribose)Polymerase activity, and by SDS-PAGE and anti-Poly(ADP-Ribose)Polymerase 1-antibodyto measure automodified Poly(ADP-Ribose)Polymerase levels bydensitometry. Poly(ADP-Ribose)Polymerase activity and PARP automodification followed the same trend. Growers daily exposed to pesticides, showed both biomarkers very high, either in the presence or in the absence of pathologies. PARP activity and auto-modification in peripheral blood lymphocytes are possible, non-invasive, androutinartools to monitor the healthy conditions of floricoltorists.

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Section: Introductionmentioning

confidence: 99%

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

Imperato

Mistretta

Marone

et al. 2019

Cells

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“…A key enzyme in the apoptotic process of cells is poly-ADP-ribose polymerase (PARP), the specific target of caspase 3 [10]. PARP is a multifaceted enzyme that through its product poly(ADP-ribose) is directly involved in the regulation of chromatin architecture and functions, with a main and prevalent role in DNA repair [11][12][13]. The synthesis of the polyanion poly(ADP-ribose) by the nuclear PARP 1, the main member of PARP family, starts after the first ADP-ribose unit has been transferred from NAD + to an acceptor protein [14].…”

Section: Introductionmentioning

confidence: 99%

A Case Study of Familiar Gastrointestinal Pathologies at Risk of Cancer. Early Detection of Degenerative Signals by Oxidative Stress Biomarkers

Ferreri¹,

Marone²,

Confalone³

et al. 2019

Preprint

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Background: Beyond risk factors such as smoking, obesity and others, gastrointestinal cancer often occurs in families and the risk of getting cancer is passed down from parents to offspring. About 5%-10% of gastrointestinal cancers are hereditary (inherited by a gene mutation from one or both parents, predisposing them to develop cancer in their lifetime). Here we describe the clinical history of family members affected by gastrointestinal pathologies which often leaded to cancer. Methods: The subjects were monitored from May 2006 to December 2017 by collecting periodically clinical and endoscopic data, and performing molecular analyses by assaying two biomarkers , auto-modification of lymphocyte Poly(ADP-ribose)Polymerase as early signal of DNA damage, and erythrocyte membrane lipid composition (Fat Profile). First we focused on the oldest members, nine brothers, and thereafter we considered their offspring. Results: Both groups of subjects developed gastrointestinal pathologies of different kind and seriousness. Some diseases evolved to cancer, sometimes as a sudden and lethal event. The results of the two molecular approaches auto-modification of Poly(ADP-ribose)Polymerase and Fat Profile), were in agreement and even predicted the clinical and imaging paths. Conclusions: Both non-invasive molecular analyses can be used preliminarly to predict altered physiological states and support clinical and imaging analyses.

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Regulatory Roles of PARP-1 and Lipids in Epigenetic Mechanisms

Faraone-Mennella¹,

Masi²,

Ferreri³

2017

Handbook of Nutrition, Diet, and Epigenetics

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Mammalian Spermatogenesis, DNA Repair, Poly(ADP-ribose) Turnover: the State of the Art

Cited by 6 publications

References 102 publications

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

Automodified Poly(ADP-Ribose) Polymerase Analysisto Monitor DNA Damagein Peripheral Lymphocytes of Floriculturists Occupationally Exposed to Pesticides

A Case Study of Familiar Gastrointestinal Pathologies at Risk of Cancer. Early Detection of Degenerative Signals by Oxidative Stress Biomarkers

Regulatory Roles of PARP-1 and Lipids in Epigenetic Mechanisms

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