Mammalian testes in organ culture

Steinberger, Anna; Steinberger, Emil; Perloff, William H.

doi:10.1016/0014-4827(64)90156-9

Cited by 119 publications

(59 citation statements)

References 11 publications

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“…However, in their systems, no haploid cells were formed. The use of bicameral chambers in the present work is most probably an important parameter explaining at least partly, the difference between our results and those of others [25][26][27][28]. Indeed, under our conditions, Sertoli cells maintained morphological features of healthy polarized Sertoli cells throughout the culture period.…”

Section: Discussioncontrasting

confidence: 64%

The Whole Meiotic Process Can Occur in Vitro in Untransformed Rat Spermatogenic Cells

Staub

Hue

Nicolle

et al. 2000

Experimental Cell Research

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The aim of the present study was to assess whether the whole meiotic process of spermatogenic cells is able to take place in vitro. Fragments of seminiferous tubules from 20-to 22-or 28-day-old rats were seeded in medium containing 0.2% fetal calf serum in bicameral chambers and then cultured for 4 weeks in a chemically defined medium. The differentiation of meiotic germinal cells was followed by four criteria: (i) ultramicroscopic examination of the different types of germ cells present in the cell layer throughout the culture period; (ii) determination of the changes in DNA content per nucleus of the cell population seeded with time in culture; (iii) assessment of the ability of germinal cells to transcribe genes expressed after completion of meiosis; and (iv) monitoring the fate of BrdU-labeled leptotene spermatocytes. The ultrastructural study showed that the overall organization of the cells in the culture well recalls that of the seminiferous epithelium throughout the culture period. Moreover the identification of young round spermatids 21 days after seeding suggested that these spermatids had been formed very recently in culture. Determination of DNA content per nucleus showed that a 1C cell population could be observed after several days of cultures reaching 6 to 10% of total cells. An exponential-like increase in the amounts of the mRNAs encoding for TP1 or TP2 occurred from the time when 1C cells appeared in the culture until the end of the experiment. Finally, BrdU-labeled leptotene spermatocytes differentiated into pachytene spermatocytes and then into secondary spermatocytes, and BdrU-labeled round spermatids were observed from Day 21 of culture onward. Taken together these results indicate that the whole meiotic process from leptotene spermatocyte to round spermatid can indeed occur in vitro under the present culture conditions.

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Section: Discussioncontrasting

confidence: 64%

The Whole Meiotic Process Can Occur in Vitro in Untransformed Rat Spermatogenic Cells

Staub

Hue

Nicolle

et al. 2000

Experimental Cell Research

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“…The method of tissue culture was described [15]. The testicular tissue fragments were cut into small pieces of approximately 1 mm3 in size.…”

Section: Methodsmentioning

confidence: 99%

Temperature Sensitivity of Human Spermatogonia and Spermatocytes in Vitro

Nakamura

Namiki

Okuyama

et al. 1987

Archives of Andrology

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To study the effect of temperature on human spermatogenesis, both the number and DNA synthesis of germ cells were investigated in tissue fragments of human testes cultured for 22 h at 3 1 "C and 37 "C. The number of differentiated germ cells such as spermatids and spermatozoa cultured at 37°C was significantly smaller than that cultured at 31 "C. The number of spermatogonia and resting primary spermatocytes was not significantly different between these two temperatures, but the functional ability of DNA synthesis in these cells was significantly lower at 37°C than at 31 "C. It seems that in normal body temperature (37 "C) differentiated germ cells such as spermatids and spermatozoa are fragile and the DNA synthesis of spermatogonia and resting primary spermatocytes is retarded.

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“…The technique of testicular tissue culture established by Steinberger et al [15] has been used mainly for histological experiments [3]. On the other hand, biochemical experiments to investigate hormonal regulation and other functions of the testes have frequently been performed using cells such as Sertoli or Leydig cells cultured in vitro.…”

Section: Discussionmentioning

confidence: 99%

Induction of Testicular DNA Synthesis In Vitro by HCG and HMG Treatments in Men with Hypogonadotropic Hypogonadism

Okuyama

Nonomura

Koh

et al. 1989

Archives of Andrology

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To study the effectiveness of hCG and hMG administration on spermatogenesis of patients with hypogonadotropic hypogonadism (HH), the DNA synthesis of spermatogonia was investigated by the method of 3H-thymidine uptake by cultured tissue fragments of human testis with or without treatment with hCG and/or hMG. Although in two groups treated with different concentrations of hCG the value of 3H-thymidine incorporation into testicular tissue showed no significant difference compared with the untreated control group, treatment with both hCG and hMG induced significant changes in the value of 3H-thymidine incorporation compared with the untreated control group. Combined hCG and hMG therapy was effective for induction of spermatogenesis in patients with HH.

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Mammalian testes in organ culture

Cited by 119 publications

References 11 publications

The Whole Meiotic Process Can Occur in Vitro in Untransformed Rat Spermatogenic Cells

The Whole Meiotic Process Can Occur in Vitro in Untransformed Rat Spermatogenic Cells

Temperature Sensitivity of Human Spermatogonia and Spermatocytes in Vitro

Induction of Testicular DNA Synthesis In Vitro by HCG and HMG Treatments in Men with Hypogonadotropic Hypogonadism

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