Mammary cell proliferation and catabolism of adipose tissues in nutrition-restricted lactating sows were associated with extracellular high glutamate levels

Zhong, Heju; Wang, Peng; Song, Yumo; Che, Lianqiang; Feng, Bin; Lin, Yan; Li, Jian; Wu, De; Wu, Qiaofeng; Fang, Zhengfeng

doi:10.1186/s40104-018-0293-6

Cited by 4 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Six primiparous sows on 60 and 90 days of pregnancy, respectively, were selected for sample collection. Mammary extracellular fluid in the left third mammary gland was collected using microdialysis method under fasting conditions on 60 and 90 days of pregnancy, respectively, and the specific operation referred to the methods we previously described in detail [11]. Mammary parenchymal tissues were collected immediately after animals were sacrificed.…”

Section: Methodsmentioning

confidence: 99%

Methionine Protects Mammary Cells against Oxidative Stress through Producing S‐Adenosylmethionine to Maintain mTORC1 Signaling Activity

Zhong

Yuan

et al. 2021

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

The mechanistic target of rapamycin complex 1 (mTORC1) signaling plays pivotal roles in cell growth and diseases. However, it remains mechanistically unclear about how to maintain mTORC1 activity during mammary glands development. Here we showed that mammary glands suffered from aggravated oxidative stress as pregnancy advanced and was accompanied by an increase in H2O2 levels, while the consumption for methionine and S-adenosylmethionine (SAM) rather than S-adenosylhomocysteine (SAH) were promoted in vivo. Likewise, H2O2 promoted SAM synthesis and reduced SAM utilization for methylation depending on H2O2 levels and treatment time in vitro. H2O2 inhibited phosphorylation of S6 kinase Thr 389 (p-S6K1 (T389)), 4E-BP1 Thr 37/46 and ULK1 Ser 757, the downstream of mTORC1, in mammary epithelial cells. However, methionine and SAM were shown to activate mTORC1 under H2O2-exposed condition. Moreover, this effect was not disabled by SGI-1027 which inhibits SAM transmethylation. In conclusion, methionine appeared to protect mammary cells against oxidative stress through producing SAM to maintain mTORC1 signaling activity.

show abstract

Section: Methodsmentioning

confidence: 99%

Methionine Protects Mammary Cells against Oxidative Stress through Producing S‐Adenosylmethionine to Maintain mTORC1 Signaling Activity

Zhong

Yuan

et al. 2021

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

show abstract

Impacts of glutamate, an exercise-responsive metabolite on insulin signaling

Xing,

Sun,

Wang

et al. 2024

Life Sciences

View full text Add to dashboard Cite

Influence of the metabolic state during lactation on milk production in modern sows

Costermans

Soede

Middelkoop

et al. 2020

Animal

View full text Add to dashboard Cite

Selection for prolificacy in sows has resulted in higher metabolic demands during lactation. In addition, modern sows have an increased genetic merit for leanness. Consequently, sow metabolism during lactation has changed, possibly affecting milk production and litter weight gain. The aim of this study was to investigate the effect of lactational feed intake on milk production and relations between mobilization of body tissues (adipose tissue or skeletal muscle) and milk production in modern sows with a different lactational feed intake. A total of 36 primiparous sows were used, which were either full-fed (6.5 kg/day) or restricted-fed (3.25 kg/day) during the last 2 weeks of a 24-day lactation. Restricted-fed sows had a lower milk fat percentage at weaning and a lower litter weight gain and estimated milk fat and protein production in the last week of lactation. Next, several relations between sow body condition (loss) and milk production variables were identified. Sow BW, loin muscle depth and backfat depth at parturition were positively related to milk fat production in the last week of lactation. In addition, milk fat production was related to the backfat depth loss while milk protein production was related to the loin muscle depth loss during lactation. Backfat depth and loin muscle depth at parturition were positively related to lactational backfat depth loss or muscle depth loss, respectively. Together, results suggest that sows which have more available resources during lactation, either from a higher amount of body tissues at parturition or from an increased feed intake during lactation, direct more energy toward milk production to support a higher litter weight gain. In addition, results show that the type of milk nutrients that sows produce (i.e. milk fat or milk protein) is highly related to the type of body tissues that are mobilized during lactation. Interestingly, relations between sow body condition and milk production were all independent of feed level during lactation. Sow management strategies to increase milk production and litter growth in modern sows may focus on improving sow body condition at the start of lactation or increasing feed intake during lactation.

show abstract

Mammary cell proliferation and catabolism of adipose tissues in nutrition-restricted lactating sows were associated with extracellular high glutamate levels

Cited by 4 publications

References 36 publications

Methionine Protects Mammary Cells against Oxidative Stress through Producing S‐Adenosylmethionine to Maintain mTORC1 Signaling Activity

Methionine Protects Mammary Cells against Oxidative Stress through Producing S‐Adenosylmethionine to Maintain mTORC1 Signaling Activity

Impacts of glutamate, an exercise-responsive metabolite on insulin signaling

Influence of the metabolic state during lactation on milk production in modern sows

Contact Info

Product

Resources

About