Entrepreneurship is related to development. Opportunity recognition is considered an important part of realizing entrepreneurial action and sustainable development. Sustainable entrepreneurship has the initial goal of entrepreneurship while still prioritizing the issues of social (human), environmental (planet) and economic (profit) concepts, but there are few published studies that empirically test the model, even though these issues are important for measuring performance. This study aims to explore the ability to recognize entrepreneurial opportunities in Rural-Owned Enterprises in terms of sustainable ecotourism in Bali. Rural-Owned Enterprises as village business institutions are established to advance village socioeconomics without compromising future generations.Data collection was carried out by in-depth interviews with seven sustainable entrepreneurs. Mixed methods were adopted to answer the research objectives. The research approach is qualitative by collecting, reducing data, and presenting data. Furthermore, the development of the questionnaire used the findings of qualitative analysis. Questionnaires were distributed to managers of Rural-owned enterprises. Thirty managers out of fifty have answered a questionnaire sent by email. Quantitatively, the data were analyzed using descriptive statistical analysis. The result of the study show that quantitatively, the managers of ecotourism Rural-Owned Enterprises on average seventy percent agree that the practice of identifying sustainable entrepreneurial opportunities is determined by 1) prior knowledge; 2) motivation; and 3) entrepreneurial knowledge. In the future, the results of this research can be further developed by linking opportunity recognition to sustainable entrepreneurship by prioritizing aspects of local culture.