Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)

Abstract

Henequen (Agave fourcroydes Lem.) is a high value plant species both for agricultural and ecological uses. The species is very well known worldwide by the quality of its fiber that is widely used for industrial purposes. This species is propagated at a large scale by asexual methods but current propagation technologies are not satisfying the grower's demands. A. fourcroydes has been cultivated in vitro but it has shown some recalcitrant behaviour during the multiplication and rooting stages. The combination of 6-benzylamynopurine (BAP) (0.75 mg L-1) and indolebutyric acid (IBA) (1.0 mg L-1) instead of 2,4-dichlorophenoxyacetic acid (2,4-D) significantly improved explant survival and shooting during the establishment of in vitro young shoots. Combining thidiazuron (TDZ) (0.5 or 0.75 mg L-1) with BAP (1.0 mg L-1) and IBA (1.0 mg L-1) in the basal medium increased the multiplication rate of henequen and significantly speeded out bud dormancy breaking. To improve rooting of the micropropagated shoots, the addition of IBA and naphthylacetic acid (NAA) was tested. The best rooting efficiency was obtained when the basal medium was supplemented with 0.5 or 0.75 mg L-1 of NAA, giving 100% of rooted explants and an average of 9.40 and 11.55 roots per explants, respectively. Over 94% of micropropagated plants survived the ex vitro weaning step and no morphological disorders were observed in any of the plants. Modification of plant growth regulators composition in the medium was a key factor to improve the efficiency of the micropropagation technology of henequen.

Meristem culture and subsequent micropropagation of Chilean strawberry (Fragaria chiloensis (L.) Duch.)

“…Although further studies are desirable, for example, to discover the levels of endogenous hormones in this species, it appears that F. chiloensis produces sufficient endogenous auxin levels to induce rooting, and that the incorporation of additional amounts lessens this process, as previously documented for sweet potato [5] and henequen [23]. …”

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

“…In those protocols, Murashige and Skoog medium has been supplemented with combinations of cytokinins and auxins such as 2,4D (2,4-dichlorophenoxyacetic acid), IBA (Indole Butyric Acid), BA (6-benzyl adenine), BAP (6-benzyl aminopurine), NAA (Naphthalene acetic acid), IAA (Indole-3-acetic acid), TDZ (Thidiazuron), Kinetin and Topolin. Among these, 2,4D-BA or 2,4D-IBA are the most commonly utilized [ 32 , 33 , 34 , 35 , 48 , 49 , 50 , 51 ].…”

ANATOMÍA Y MORFOLOGÍA DE PLANTAS MICROPROPAGADAS-ACLIMATADAS DE Agave potatorum Zucc. FERTIRRIGADAS EN VIVERO

“…Hay antecedentes de micropropagación de A. fourcroydes Lem. (Garriga et al, 2010), A. tequilana Weber (Angeles-Espino et al, 2012), A. grijalvensis B. Ullrich (Santíz et al, 2012), A. angustifolia Haw. (Monja-Mio et al, 2015), que describen los procedimientos de propagación in vitro, la transferencia de las plantas a sustrato e invernadero para su aclimatización.…”