Management of cutaneous T cell lymphoma

The spectrin repeat folds into a three‐helix bundle in solution

“…The equivalent residues in the crystal structure (from Gln-71 to Ala-75) are in a helical conformation (~ =-64.24 and =-43.86 on average). Regarding the tertiary structure, the interhelical NOEs indicate that the helix packing is similar to that observed in the crystal structure of the dimer, although the ongoing structure determination still has to distinguish properly between the tertiary structure of the two different models, either a left-handed coiled-coil [16] or a left-handed three-helix bundle [18]. The elucidation of the three-dimensional structure of the spectrin repeat is a step towards a fuller understanding of the structure and dynamics of the membrane skeleton.…”

“…troscopy and protease resistance have led to the determination of the domain boundaries (phasing) and have shown that the repeat is an independent folding unit with a high helical content [13][14][15]. Structural models for the repeat predict a lefthanded coiled-coil made by three a-helices (A-C) separated by two short loops (AB, BC) [16], although the probability for a coiled-coil fold according to the method of [17] is low. However, the X-ray structure of the 14th repeat from the Drosophila cx-spectrin [18] shows a homodimer which has a different secondary and tertiary structure compared to that of the proposed model [16].…”

“…Structural models for the repeat predict a lefthanded coiled-coil made by three a-helices (A-C) separated by two short loops (AB, BC) [16], although the probability for a coiled-coil fold according to the method of [17] is low. However, the X-ray structure of the 14th repeat from the Drosophila cx-spectrin [18] shows a homodimer which has a different secondary and tertiary structure compared to that of the proposed model [16]. At the secondary structure level, helices B and C are continuous and the proposed BC loop is in fact helical.…”