Management ofMycobacterium aviumsubsp.paratuberculosisin dairy farms:Selection and evaluation of different DNA extraction methods from bovine and buffaloes milk and colostrum for the establishment of a safe colostrum farm bank

Gamberale, F.; Pietrella, Gabriele; Sala, M.; Scaramella, Paola; Puccica, Silvia; Antognetti, Valeria; Arrigoni, N.; Ricchi, M.; Cersini, Antonella

doi:10.1002/mbo3.875

Cited by 7 publications

(9 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In buffalo, as in cattle, infection/exposure to NTM can interfere with bTB diagnosis, because the composition of PPDs includes several antigens that can cross-react with environmental mycobacteria and this may lead to false positive reactions (93). MAP, the causative agent of PTB, is one of the most important NTM causes of false positive reactions to PPD in cattle and buffalo (11,12,(69)(70)(71)(72)(73)(74)(75).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo (Bubalus bubalis) Comparing Four Interpretative Criteria

et al. 2020

Self Cite

View full text Add to dashboard Cite

Bovine tuberculosis (bTB) is a worldwide zoonosis that affects many species of domestic and wild animals. Mycobaterium bovis is the main cause of infection in water buffalo (Bubalus bubalis) and bovines and is of great concern for human health and for buffalo producers in Italy. The bTB eradication programme is based on slaughterhouse surveillance and intradermal skin tests. Other in vivo diagnostic methods such as the interferon-gamma (IFN-γ) assay have been developed and are widely used in cattle to accelerate the elimination of bTB positive animals. The present study is the first to assess the use and performance of IFN-γ assays, which is used as an ancillary test for bTB diagnosis in water buffalo, and presents the results of a field-evaluation of the assay from 2012 to 2019 during the buffalo bTB eradication programme in Italy. The study involved 489 buffaloes with a positive result to the single intradermal tuberculin test (SITT). The IFN-γ assays and single intradermal comparative tuberculin test were used as confirmation tests. Then, a total of 458 buffaloes, reared on officially tuberculosis-free (OTF) herds, that were confirmed bTB-free for at least the last 6 years were subjected to IFN-γ testing. Furthermore, to evaluate the IFN-γ test in an OTF herd with Paratuberculosis (PTB) infection, 103 buffaloes were subjected to SITT and IFN-γ test simultaneously. Four interpretative criteria were used, and the IFN-γ test showed high levels of accuracy, with sensitivity levels between 75.3% (CI 95% 71.2–79.0%) and 98.4% (CI 95% 96.7–99.4%) and specificity levels between 94.3% (CI 95% 91.2–96.50%) and 98.5% (CI 95% 96.9–99.4%), depending on the criterion used. Finally, in the OTF herd with PTB infection, in buffalo, the IFN-γ test displayed high specificity values according to all 4 interpretative criteria, with specificity levels between 96.7% (CI 95% 88.4–99.5%) and 100% (CI 95% 96.2–100%), while SITT specificity proved unsatisfactory, with a level of 45.3% (CI 95% 35.0–55.7%). Our results showed that the IFN-γ test in the buffalo species could reach high Sensitivity and Specificity values, and that the level of Sensitivity and Specificity could be chosen based on the interpretative criterion and the antigens used depending on the health status of the herd and the epidemiological context of the territory. The IFN-γ test and the use of different interpretative criteria proved to be useful to implement bTB diagnostic strategies in buffalo herds, with the possibility of a flexible use of the assay.

show abstract

Section: Discussionmentioning

confidence: 99%

“…As NTM can interfere with bTB diagnosis (16, 41), we wanted to evaluate the performance of SITT and IFN-γ in the presence of NTM, in particular MAP, that could be present in buffalo herds (41, [69][70][71][72][73][74][75].…”

Section: Assessment Of the Performance Of Sitt And Ifn-γ On A Tubercumentioning

confidence: 99%

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo (Bubalus bubalis) Comparing Four Interpretative Criteria

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Only one sample was M. bovis positive by the enriched-broth PCR but negative by the direct-milk PURE-LAMP. Colostrum contains many components, such as fat and protein, which lower the accuracy of DNA extraction compared with mature milk [ 3 ]. The transitional milk used in this study was obtained from the second sample after parturition and, as such, was the second most concentrated milk after colostrum.…”

Section: Discussionmentioning

confidence: 99%

Combination of procedure for ultra rapid extraction (PURE) and loop-mediated isothermal amplification (LAMP) for rapid detection of Mycoplasma bovis in milk

Itoh

Hirano

Yamakawa³

et al. 2020

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Polymerase chain reaction (PCR) is typically used for the early detection of mycoplasma in bovine milk; it requires 3 days to obtain results because of the necessary enrichment process. A more rapid, simple, and accurate detection method is required to directly detect the Mycoplasma bovis ( M. bovis ) gene in milk. In this study, we assess the utility of combining the following two methods to achieve this goal: the loop-mediated isothermal amplification (LAMP), which is more sensitive than PCR, and the procedure for ultra rapid extraction (PURE), which adsorbs and filters components that inhibit DNA amplification/detection. LAMP was examined using DNA extracts obtained by four methods. This showed that PURE had the highest sensitivity and specificity and that the combination of PURE and LAMP was able to detect M. bovis in milk. We then showed that the detection limit of M. bovis was 10 2 colony-forming units per milliliter of milk using the PURE–LAMP. Finally, the respective sensitivities of the PURE–LAMP and PCR were 57% and 86% for bulk tank milk, 89% and 74% for mature milk, 85% and 92% for colostrum/transitional milk, and 97% and 95% for mastitis milk. The specificity was 100% for all milk samples in both LAMP and PCR. We conclude that PCR was suitable for detecting mycoplasma in bulk tank milk and that the PURE–LAMP could detect mycoplasma within 2 hr and was also effective for mature and mastitis milk.

show abstract

“…In the dairy herd, MAP is transmitted horizontally primarily by the fecal–oral route and vertically in utero, in the milk and colostrum (as determined via PCR) to newborn calves. The colostrum is an important source of growth factors, vitamins, and immunoglobulins (IgGs) as calves have agammaglobulinemia at birth [ 24 ] and require these IgGs via colostrum. As such, animals become infected within the first month of life with bovine susceptibility to MAP infection decreasing with age [ 25 ].…”

Section: The Triad Of Johne’s Diseasementioning

confidence: 99%

“…IS900 is the molecular target for PCR amplification, however, this sequence can be detected in other Mycobacterium species, leading to false positives. Cultural isolation of MAP is considered the gold standard for detection of the pathogen with a sensitivity of less than 30% and specificity of 100% in subclinically infected cattle [ 24 ]. The culture of fecal MAP is dependent on the stage of disease as the pathogen is excreted intermittently and to varying extents depending on the animal.…”

Section: Prevalence Of Diseasementioning

confidence: 99%

Mycobacterium Avium Paratuberculosis: A Disease Burden on the Dairy Industry

Garvey

2020

Animals

View full text Add to dashboard Cite

Mycobacterium avium paratuberculosis is responsible for paratuberculosis or Johne’s disease in cows, having economic impacts on the dairy industry and a prevalence rate exceeding 50% in dairy herds. The economic burden of Johne’s disease relates to decreased milk production and costs of disease prevention, treatment, and management, while having an economic impact on dairy producers, processors, consumers, and stakeholders of the dairy industry. Determining the true economic impact of the disease is difficult at regional and farm level as symptoms are not evident in subclinically infected animals. At present, the virulence, pathogenicity, persistence, and infectious dose of M. avium paratuberculosis are poorly understood, consequently effective paratuberculosis control measures remain obscure. M. avium paratuberculosis is potentially zoonotic with foodborne transmission a public health risk due to a possible causative link with inflammatory bowel disease in humans. A preventive approach is necessary to reduce the presence of this drug-resistant pathogen in dairy herds and subsequently dairy food. The use of inefficient diagnostic tests coupled with the long latency period of infection results in delayed animal culling and trade of asymptomatic animals, leading to regional transmission and increased disease prevalence. To date, there has been limited success at controlling and treating this terminal endemic disease, leading to significant prevalence rates. This study aims to outline the key factors associated with Johne’s’ disease while outlining its significant impact on the dairy sector.

show abstract

Management ofMycobacterium aviumsubsp.paratuberculosisin dairy farms:Selection and evaluation of different DNA extraction methods from bovine and buffaloes milk and colostrum for the establishment of a safe colostrum farm bank

Cited by 7 publications

References 44 publications

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo (Bubalus bubalis) Comparing Four Interpretative Criteria

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo (Bubalus bubalis) Comparing Four Interpretative Criteria

Combination of procedure for ultra rapid extraction (PURE) and loop-mediated isothermal amplification (LAMP) for rapid detection of <i>Mycoplasma bovis</i> in milk

Mycobacterium Avium Paratuberculosis: A Disease Burden on the Dairy Industry

Contact Info

Product

Resources

About