Management of Microbiological Hazards

Jackson, T.

doi:10.1016/b978-0-12-381504-0.00033-0

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…As such, it has been suggested as an alternative to TVC that can provide accurate results in a shorter time. Recent study has shown multiplex qPCR to be capable of simultaneously detecting up to 12 important foodborne pathogens in a single assay (Tang, 2023).…”

Section: Qrt-pcr In Detecting Salmonella Spmentioning

confidence: 99%

Molecular Identification and Characterization of Salmonella Sp. In Raw and Heat-Treated Milks from Local Market

Rashad,

Refaat,

Ismail

et al. 2023

Annals of Agricultural Science, Moshtohor

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Milk is an excellent nutritious food that is characterized by a short shelf life and requires careful handling. Therefore, milk is rapidly perishable as it is a suitable medium for the pathogenic bacterial growth especially; causing milk spoilage and diseases in consumers. Nine Salmonella isolates recovered from 4 (8%) and 5 (10%) samples of Raw cow milk and Raw buffalo milk, respectively while no isolates of salmonella sp. were detected from heat-treated milk. Moreover, the antimicrobial susceptibility testing showed that nine isolates were resistant to Amoxicillin/clavulanic acid (AMC 30) 20/10μg, Erythromycin (E15) 15μg and Cephalexin (CL30) 30μg whilst they are all sensitive to Chloramphenicol (C30) 30μg. Additionally, our isolates revealed that they were posed 16S rRNA gene and by using amplified ribosomal DNA restriction analyses (ARDRA) technique the resulting restriction fragment patterns, showing that all isolates following the same genus and species. Importantly, two isolates were sequenced and submitted in NCBI under accession numbers OR048345 and OR102504. The virulence gene profile of the nine isolates revealed that they are poses the six virulence genes (invA, orgA, sifA, spvA, iroN and spiA) were detected significantly in all nine isolates. A mimic experiment was done to estimate the sensitivity of the real time-PCR to detect salmonella sp. in contaminated milk samples. Our results indicate that insufficient hygienic measures adopted during handling. Therefore, the strict hygienic approaches are recommended during milking and processing. The current study aimed to determine the prevalence of Salmonella sp. in raw and heat-treated milk, characterize its virulence-associated genes, and assess its antimicrobial profile.

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Section: Qrt-pcr In Detecting Salmonella Spmentioning

confidence: 99%

Molecular Identification and Characterization of Salmonella Sp. In Raw and Heat-Treated Milks from Local Market

Rashad,

Refaat,

Ismail

et al. 2023

Annals of Agricultural Science, Moshtohor

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Environmental monitoring program to support food microbiological safety and quality in food industries: A scoping review of the research and guidelines

et al. 2021

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Evaluation of an Environmental Monitoring Program for the Microbial Safety of Air and Surfaces in a Dairy Plant Environment

Zacharski

Southern

Ryan

et al. 2018

Journal of Food Protection

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Microbiological hazards can occur when foodstuffs come into contact with contaminated surfaces or infectious agents dispersed by air currents in the manufacturing environment. An environmental monitoring program (EMP) is a critical aspect of sustainable and safe food manufacturing used to evaluate the effectiveness of the microbial controls in place. An effective EMP should be based on risk analysis, taking into account previous sampling history to determine the selection of the sampling points, the scope of the test, and the frequency of analysis. This study involved evaluation of the environmental monitoring regime and microbiological status of a medium-sized dairy plant manufacturing food ingredients, e.g., proteins, milk powders, and dairy fats. The data specific to microbial tests ( n = 3,468), recorded across 124 fixed sampling locations over a 2-year period (2014 to 2015) from air ( n = 1,787) and surfaces ( n = 1,681) were analyzed. The aim of this study was to highlight the strengths and weaknesses of the EMP in a select dairy processing plant. The results of this study outline the selection of sampling locations, the scope of the test, and the frequency of analysis. An analysis of variance revealed subsections of the manufacturing areas with high risk factors, especially the packaging subsection specified for bulk packaging, the atomizer, and the fluidized bed. The temporal and spatial analysis showed the potential to reduce or relocate the monitoring effort, most notably related to total coliforms and Staphylococcus aureus, across the dairy plant due to homogeneity across the sampling subsections with little or no deviations. The results suggest a need to reevaluate the current EMP and the corrective action plan, especially with regard to detection of pathogens. Recommendations for optimization of the EMP are presented to assist the dairy industry with reviewing and revising the control measures and hazard assessment with regard to existing contamination issues.

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Management of Microbiological Hazards

Cited by 3 publications

References 14 publications

Molecular Identification and Characterization of Salmonella Sp. In Raw and Heat-Treated Milks from Local Market

Molecular Identification and Characterization of Salmonella Sp. In Raw and Heat-Treated Milks from Local Market

Environmental monitoring program to support food microbiological safety and quality in food industries: A scoping review of the research and guidelines

Evaluation of an Environmental Monitoring Program for the Microbial Safety of Air and Surfaces in a Dairy Plant Environment

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