Zebarth, B. J., Botha, E. J. and Rees, H. 2007. Rate and time of fertilizer nitrogen application on yield, protein and apparent efficiency of fertilizer nitrogen use of spring wheat. Can. J. Plant Sci. 87: 709-718. Use of an in-season measurement of crop nitrogen (N) status to optimize fertilizer N management has been proposed as a means of optimizing yield of spring wheat while minimizing environmental N losses. This study determined the effect of the rate and time of fertilizer N application on the grain yield, grain protein, and apparent recovery of fertilizer N in grain and in the above-ground plant for spring wheat (Triticum aestivum L.) in [2001][2002][2003], and evaluated the use of a SPAD-502 meter to measure crop N status in spring wheat. Sixteen N fertility treatments were used, including application of different rates of fertilizer N (0-160 kg N ha -1 ) applied pre-seeding (ZGS 0), at tillering (ZGS 21) and at shooting (ZGS 32) as ammonium nitrate. Split N application provided no benefit in terms of grain yield or apparent recovery of fertilizer N. Application of fertilizer N at ZGS 32 reduced crop yield and apparent recovery of fertilizer N compared with N application at ZGS 0. Application of fertilizer N at ZGS 21 reduced yield and apparent recovery of fertilizer N in grain in 2 of 3 yr, but had no effect on apparent recovery of fertilizer N in the above-ground plant. Delayed fertilizer N application generally increased grain protein. Fertilizer N can be applied at ZGS 21 as required to optimize grain yield provided at least some fertilizer N is applied prior to seeding; however, crop N status cannot reliably be assessed at this time using a SPAD-502 meter. Crop N status can be assessed at ZGS 32 using a SPAD-502 meter; however, fertilizer N application at this time primarily influences grain protein rather than grain yield. These results highlight the need for a means of predicting soil N mineralization potential in order to optimize grain yield in humid environments where carry-over of soil nitrate from the previous growing season is limited. . L'application fractionnée d'engrais N n'améliore pas le rendement grainier ni la récupéra-tion apparente du N. L'application de l'engrais à ZGS 32 diminue le rendement et la récupération apparente du N comparativement à ce qu'on observe quand l'application a lieu à ZGS 0. Appliquer l'engrais à ZGS 21 a aussi diminué le rendement et la récupération apparente du N par le grain deux années sur trois, mais n'a eu aucune incidence sur la récupération apparente du N par les organes aériens de la plante. Retarder l'application de l'engrais accroît généralement la concentration de protéines dans le grain. On peut appliquer la quantité requise d'engrais à ZGS 21 pour optimiser le rendement grainier pourvu qu'une partie de l'engrais soit appliquée avant les semis, mais il sera impossible d'évaluer le bilan azoté de manière fiable avec le compteur SPAD-502. L'application d'engrais N à ce moment influe toutefois plus sur la concentration en protéines du grain que sur l...