Management of Stricture Urethra: Our Experience at a Tertiary Care Centre

Kumar, N Anil; Chaitanya, S Venkata; Pinni, Suraj; Goyal, Naveen; Sharma, Tushar; Yagneshwar, Sharma A

doi:10.21276/ijcmr.2020.7.4.19

IJCMR

2020

DOI: 10.21276/ijcmr.2020.7.4.19

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Management of Stricture Urethra: Our Experience at a Tertiary Care Centre

N Anil Kumar¹,

S Venkata Chaitanya²,

Suraj Pinni³

et al.

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2024

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Low-intensity pulsed ultrasound promotes cell viability of hUSCs in volumetric bioprinting scaffolds via PI3K/Akt and ERK1/2 pathways

Chen,

Li,

Dai

et al. 2024

Biomed. Mater.

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Objective:The study aimed to investigate the impact of low-intensity pulsed ultrasound (LIPUS) on human urinary-derived stem cells (hUSCs) viability within 3D cell-laden gelatin methacryloyl (GelMA) scaffolds.Methods:hUSCs were integrated into GelMA bio-inks at concentrations ranging from 2.5% to 10% (w/v) and then bioprinted using a volumetic-based method. Subsequent exposure of these scaffolds to LIPUS under varying parameters or sham irradiation aimed at optimizing the LIPUS treatment. Assessment of hUSCs viability employed Cell Counting Kit-8 (CCK8), cell cycle analysis, and live&dead cell double staining assays. Additionally, Western blot analysis was conducted to determine protein expression levels.Result:With 3D bio-printed cell-laden GelMA scaffolds successfully constructed, LIPUS promoted the proliferation of hUSCs. Optimal LIPUS conditions, as determined through CCK8 and live&dead cell double staining assays, was achieved at a frequency of 1.5 MHz, a spatial-average temporal-average intensity (ISATA) of 150 mW/cm2, with an exposure duration of 10 minutes per session administered consecutively for two sessions. LIPUS facilitated the transition from G0/G1 phase to S and G2/M phases and enhanced the phosphorylation of ERK1/2 and PI3K-Akt. Inhibition of ERK1/2 (U0126) and PI3K (LY294002) significantly attenuated LIPUS-induced phosphorylation of ERK1/2 and PI3K-Akt respectively, both of which decreased the hUSC viability within 3D bio-printed GelMA scaffolds.Conclusion:Applying a LIPUS treatment at an ISATA of 150 mW/cm2 promotes the growth of hUSCs within 3D bio-printed GelMA scaffolds through modulating ERK1/2 and PI3K-Akt signaling pathways.

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Low-intensity pulsed ultrasound promotes cell viability of hUSCs in volumetric bioprinting scaffolds via PI3K/Akt and ERK1/2 pathways

Chen,

Li,

Dai

et al. 2024

Biomed. Mater.

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show abstract

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Management of Stricture Urethra: Our Experience at a Tertiary Care Centre

Cited by 1 publication

References 0 publications

Low-intensity pulsed ultrasound promotes cell viability of hUSCs in volumetric bioprinting scaffolds via PI3K/Akt and ERK1/2 pathways

Low-intensity pulsed ultrasound promotes cell viability of hUSCs in volumetric bioprinting scaffolds via PI3K/Akt and ERK1/2 pathways

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