Skin swabs collected from (60) patients attending the Dermatology Clinic at Madinah General Hospital and Al-Fayhaa General Hospital in Basrah province from October 2021 to March 2022 were surveyed for the Prescence of dermatomycosis. Direct microscopical examination was carried out with 15% KOH, and cultures were performed in petri dishes on Sabouraud`s Dextrose Agar with Chloramphenicol. Among the 55 samples, only two cases was assigned to superficial mycoses one in a 45-years-old woman and the other case was assigned to superficial mycoses in a 23-years-old woman. The first causal agent was identified as Hanseniaspora opuntiae HAM17 and the second causal agent was identified as Cutaneotrichosporon mucoides HAM14. The yeasts were diagnosed then the cultures were examined and purified for phenotypic and genetic diagnosis using primers ITS1-ITS4 and registered in the Japanese Genbank as new strains with accession number LC722487 for H. opuntiae HAM17, and LC722484 for C. mucoides HAM14. From follow-up research and studies around the world, it appears that the current record represents the first case of isolation of H. opuntiae from superficial mycoses infection of a diabetic patient and a fourth case from clinical specimens.The reported specieses is newly recorded in Iraq.