Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Kepler, Ryan M.; Maul, Jude E.; Rehner, Stephen A.

doi:10.1016/j.mib.2017.03.006

Cited by 58 publications

(33 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous literature pertaining to Beauveria indicated that these fungi may not be active per se in rhizosphere soils, in comparison with species of Metarhizium ( Bruck, 2010 ; Kepler et al, 2017 ). However, in our study B. bassiana was more frequently detected in rhizosphere soil when plants were subjected to wound stress, which suggests that these fungi may be functional in the rhizosphere when facilitated by the host plant.…”

Section: Discussionmentioning

confidence: 96%

Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants

et al. 2018

View full text Add to dashboard Cite

Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize a broad range of plant hosts as endophytes without causing disease but while still maintaining the capacity to infect insects. Beauveria is often applied as an inundative spore application, but little research has considered how plant colonization may alter the ability to persist in the environment. The aim of this study was to investigate potential interactions between B. bassiana and Zea mays L. (maize) in the rhizosphere following inoculation, in order to understand the factors that may affect environmental persistence of the fungi. The hypothesis was that different isolates of B. bassiana have the ability to colonize maize roots and/or rhizosphere soil, resulting in effects to the plant microbiome. To test this hypothesis, a two-step nested PCR protocol was developed to find and amplify Beauveria in planta or in soil; based on the translation elongation factor 1-alpha (ef1α) gene. The nested protocol was also designed to enable Beauveria species differentiation by sequence analysis. The impact of three selected B. bassiana isolates applied topically to roots on the rhizosphere soil community structure and function were consequently assessed using denaturing gradient gel electrophoresis (DGGE) and MicroRespTM techniques. The microbial community structure and function were not significantly affected by the presence of the isolates, however, retention of the inocula in the rhizosphere at 30 days after inoculation was enhanced when plants were subjected to intensive wounding of foliage to crudely simulate herbivory. The plant defense response likely changed under wound stress resulting in the apparent recruitment of Beauveria in the rhizosphere, which may be an indirect defensive strategy against herbivory and/or the result of induced systemic susceptibility in maize enabling plant colonization.

show abstract

Section: Discussionmentioning

confidence: 96%

Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Fungi from the ascomycete class Sordariomycetes were notably shared between the two coffee systems and included orders such as Hypocreales. One of the richest sources of biocontrol fungi have been from the order Hypocreales [62], with several strains commercially produced such as Metarhizium (Met52, Novozymes Biologicals) used as a bioinsecticide and Trichoderma harzianum (T-22 HC, BioWorks) used to control soil-borne diseases. We found a Table 3.…”

Section: Plos Onementioning

confidence: 99%

Soil fungal communities differ between shaded and sun-intensive coffee plantations in El Salvador

et al. 2020

View full text Add to dashboard Cite

Coffea arabica is a highly traded commodity worldwide, and its plantations are habitat to a wide range of organisms. Coffee farmers are shifting away from traditional shade coffee farms in favor of sun-intensive, higher yield farms, which can impact local biodiversity. Using plant-associated microorganisms in biofertilizers, particularly fungi collected from local forests, to increase crop yields has gained traction among coffee producers. However, the taxonomic and spatial distribution of many fungi in coffee soil, nearby forests and biofertilizers is unknown. We collected soil samples from a sun coffee system, shade coffee system, and nearby forest from Izalco, Sonsonate, El Salvador. At each coffee system, we collected soil from the surface (upper) and 10 cm below the surface (lower), and from the coffee plant drip line (drip line) and the walkway between two plants (walkway). Forest soils were collected from the surface only. We used ITS metabarcoding to characterize fungal communities in soil and in the biofertilizer (applied in both coffee systems), and assigned fungal taxa to functional guilds using FUNGuild. In the sun and shade coffee systems, we found that drip line soil had higher richness in pathotrophs, symbiotrophs, and saprotrophs than walkway soil, suggesting that fungi select for microhabitats closer to coffee plants. Upper and lower soil depths did not differ in fungal richness or composition, which may reflect the shallow root system of Coffea arabica. Soil from shade, sun, and forest had similar numbers of fungal taxa, but differed dramatically in community composition, indicating that local habitat differences drive fungal species sorting among systems. Yet, some fungal taxa were shared among systems, including seven fungal taxa present in the biofertilizer. Understanding the distribution of coffee soil mycobiomes can be used to inform sustainable, ecologically friendly farming practices and identify candidate plant-growth promoting fungi for future studies.

show abstract

“…Most ascomycetes affiliated to the order Hypocreales (seven out of ten) were less abundant in the rhizosphere of the wildtype compared with max4. This order includes typical plant pathogens with wide host spectra (Coleman, 2016;Kepler et al, 2017;Lombard et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

The ability of plants to produce strigolactones affects rhizosphere community composition of fungi but not bacteria

et al. 2019

View full text Add to dashboard Cite

Managing the plant microbiome for biocontrol fungi: examples from Hypocreales

Cited by 58 publications

References 83 publications

Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants

Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants

Soil fungal communities differ between shaded and sun-intensive coffee plantations in El Salvador

The ability of plants to produce strigolactones affects rhizosphere community composition of fungi but not bacteria

Contact Info

Product

Resources

About