Hosting up to 3.3 billion barrels of oil in place, the upfaulted Precambrian crystalline rocks of the Lancaster field, offshore west of Shetland, give key insights into how fractured hydrocarbon reservoirs can form in such old rocks. The Neoarchean (c. 2700–2740 Ma) charnockitic basement is cut by deeply penetrating oil-, mineral- and sediment-filled fissure systems seen in geophysical and production logs and thin sections of core. Mineral textures and fluid inclusion geothermometry suggest that a low-temperature (<200°C) near-surface hydrothermal system is associated with these fissures. The fills help to permanently prop open fissures in the basement, permitting the ingress of hydrocarbons into extensive well-connected oil-saturated fracture networks. U–Pb dating of calcite mineral fills constrains the onset of mineralization and contemporaneous oil charge to the mid-Cretaceous and later from Jurassic source rocks flanking the upfaulted ridge. Late Cretaceous subsidence and deposition of mudstones sealed the ridge, and was followed by buoyancy-driven migration of oil into the pre-existing propped fracture systems. These new observations provide an explanation for the preservation of intra-reservoir fractures (‘joints’) with effective apertures of 2 m or more, thereby highlighting a new mechanism for generating and preserving fracture permeability in sub-unconformity fractured basement reservoirs worldwide.Supplementary material: Analytical methods and isotopic compositions and ages are available at https://doi.org/10.6084/m9.figshare.c.4763237Thematic Collection: This article is part of the Geology of Fractured Reservoirs collection available at: https://www.lyellcollection.org/cc/the-geology-of-fractured-reservoirs