Mandarin essential oils inhibit quorum sensing and virulence factors of Pseudomonas aeruginosa

Luciardi, María Constanza; Blázquez, María Amparo; Cartagena, Elena; Bardón, Alicia; Arena, Mario Eduardo

doi:10.1016/j.lwt.2015.12.056

Cited by 74 publications

(45 citation statements)

References 48 publications

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“…Lemon EO was capable to inhibit E. coli and mixed-culture biofilms; on the other hand, cinnamon was effective against the mixed forms [75]. Conversely, the entire EO of tangerine (Citrus reticulata) is capable to inhibit the P. aeruginosa biofilm formation more than its main component limonene, by an inhibition of the QS autoinducer production and elastase activity [76]. This also highlights how, within a same genus, not all the species show the same biological activity.…”

Section: Effect Of Essential Oils On Microbial Cells (Modified Frommentioning

confidence: 97%

Essential Oils and Microbial Communication

Nazzaro¹,

Fratianni²,

d’Acierno³

et al. 2020

Essential Oils - Oils of Nature

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The World Health Organization highlighted the increase in the resistance to conventional antibiotics for most pathogens and observed also a decrease of the threshold for all mechanisms of cell-cell microbial communication, leading to the formation of biofilms and to the increase of microbial pathogenicity. Scientific community is therefore oriented to the identification and study of alternative substances to antibiotics. In such context, substances of vegetal source, such as essential oils (EOs), always used in traditional medicine, stimulated-particularly in recent decades-the scientific world to discover and identify substances, intended as a mixture or single components capable to fight pathogenic microorganisms. From this point of view, the study of plants is very interesting and offers many interesting ideas and results. This brief chapter describes the basis of the microbial communication, until the formation of biofilm, and some mechanisms through which essential oils, or some of their main components, may decrease or inactivate the complex mechanisms that lead to pathogenicity, both of prokaryotes and eukaryotes.

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Section: Effect Of Essential Oils On Microbial Cells (Modified Frommentioning

confidence: 97%

Essential Oils and Microbial Communication

Nazzaro¹,

Fratianni²,

d’Acierno³

et al. 2020

Essential Oils - Oils of Nature

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“…Unlike antibiotics, whose purpose is to destroy bacteria, which leads to the appearance of resistance, attenuation of the virulence via a QS disruptor might offer a new alternative for the development of new antimicrobial molecules; even if there are no actual proof that it’ll reduce the risk of resistance apparition [ 21 ]. Some EOs like Mandarin or Marjoram have already been tested in the food industry and are known to have an anti-QS effect by preventing biofilm formation [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Quorum Sensing Activity of 12 Essential Oils on chromobacterium violaceum and Specific Action of cis-cis-p-Menthenolide from Corsican Mentha suaveolens ssp. Insularis

et al. 2018

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Quorum sensing (QS) is a bacterial communication mechanism used to express various survival or virulence traits leading to enhanced resistance. Chromobacterium violaceum is a commonly used strain that highlights anti-QS action of bioactive substances. Here, we wanted to see if 12 selected essential oils (EO) could exert anti-QS activity. We measured the sublethal minimal QS inhibitory concentration (MQSIC) by assessing violacein production of C. violaceum along with bacterial growth. To confirm the QS disruption, we also proceed to surface bacterial observations using scanning electron microscopy (SEM). We showed that cis-cis-p-menthenolide extracted and isolated from a plant endemic to occidental Mediterranean Sea islands, Mentha suaveolens ssp. insularis, acts as an inhibitor of violacein production and biofilm formation. Measured MQSIC was much lower than the minimal inhibitory concentration (MIC): 0.10 mg·mL−1 vs. 3.00 mg·mL−1. Moreover, disturbance of QS-related traits was confirmed by the degradation of C. violaceum biofilm matrix. There is a clear structure–activity relationship between cis-cis-p-menthenolide and anti-QS activity. Indeed, its isomer molecule (mintlactone) exerts a poor anti-QS action. These results indicate that inhibition of violacein production and biofilm formation by cis-cis-p-menthenolide might be related to a disruption in the QS mechanism.

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“…In addition, the influence of few secondary metabolites from South American plants on biofilm formation has been reported by our laboratory [41,43,44,45,46]. Moreover, several food components such as honey [47], Zuccagnia punctata [48] or citrus essential oil [19] are able to inhibit P. aeruginosa biofilm.…”

Section: Column Chromatographic Of L Integrifoliamentioning

confidence: 85%

“…For biofilm quantification, a micro-method based on a protocol previously reported was employed [18] with some modifications [19].…”

Section: Biofilm Formation Assaymentioning

confidence: 99%

Anti-Pathogenic Activity of Herbs Used in Argentinean Traditional Infusion

Brodkiewicz

Molina

López

et al. 2017

Glob. J. Agric. Innov. Res. Dev

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The anti-pathogenic activity of Acantholippia deserticola, Haplopappus baylahuen, Lippia integrifolia and Satureja parvifolia herbs used as traditional infusion was investigated. The extracts obtained were compared with respect to chromatographic profiles and antimicrobial activities. The dichloromethane and methanol extracts of the four herbs showed significant inhibition of Staphylococcus aureus and Pseudomonas aeruginosa growth and biofilm formation. The dichloromethane extract of Lippia integrifolia that showed the highest inhibitory effect on P. aeruginosa biofilm formation, was fractionated by column chromatography using a gradient of polarity, and the activities of the fractions were evaluated. In general, the lower polar fractions inhibited biofilm in correlation with bacterial growth. However, in more polar fractions the biofilm diminution is well correlated with the inhibition of autoinducers production more than the bacterial development. The results provide scientific support for the usage of these herbs to the protection against foodborne diseases. This effect is noteworthy in L. integrifolia because the fractions showed higher growth, biofilm, and autoinducer inhibitory activity than the crude extract.

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Mandarin essential oils inhibit quorum sensing and virulence factors of Pseudomonas aeruginosa

Cited by 74 publications

References 48 publications

Essential Oils and Microbial Communication

Essential Oils and Microbial Communication

Anti-Quorum Sensing Activity of 12 Essential Oils on chromobacterium violaceum and Specific Action of cis-cis-p-Menthenolide from Corsican Mentha suaveolens ssp. Insularis

Anti-Pathogenic Activity of Herbs Used in Argentinean Traditional Infusion

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