“…126-127°C) of the last one are in agreement with the literature data for cyperotundone (12,13). The 13 C-NMR data together with the 13 C-1 H long-range correlations in the HMBC spectrum are showed on Table II.…”
The volatiles of Cyperus giganteus (Cyperaceae), cultivated in the state of Pará were obtained by hydrodistillation and analyzed by GC and GC/MS. The major components identified in the rhizome oil of C. giganteus were cyperotundone (25.9%) and cyperene (10.4%).
“…126-127°C) of the last one are in agreement with the literature data for cyperotundone (12,13). The 13 C-NMR data together with the 13 C-1 H long-range correlations in the HMBC spectrum are showed on Table II.…”
The volatiles of Cyperus giganteus (Cyperaceae), cultivated in the state of Pará were obtained by hydrodistillation and analyzed by GC and GC/MS. The major components identified in the rhizome oil of C. giganteus were cyperotundone (25.9%) and cyperene (10.4%).
“…The DEPT experiment showed five methines, two methylenes, four methyls and three quaternary carbons. When the spectral data was compared with reported NMR and other spectral data for the sesquiterpene mustakone (Nyasse et al, 1988), significant similarity was observed except that instead of the isopropyl at C-7 of mustakone, present compound contain isopropyl alcohol moiety at the same position which was further confirmed by quaternary carbon signal at ı C 72.8. Hence, the structure of compound was deduced as 11-hydroxymustakone (3).…”
“…The 13 C NMR spectrum (ESM Table S1) showed the signals of the 15 carbon atoms amongst others. Comparing the 1 H and 13 C NMR data of mustakone ( 1 ) [6, 7] with those of AMJ1, further confirmed the structure, mustakone which was previously isolated from the rhizomes of C. articulatus [7] was the major compound.Fig. 2Effect of compounds from C. articulatus essential oil (hexane extract) on O. ochengi microfilariae (MF), adult male (AM) and adult female (AF) worms after 20 h incubation.…”
The aims of this investigation were to isolate active ingredients from the roots/rhizomes of Cyperus articulatus used as herbal medicine in Cameroon for the treatment of human onchocerciasis and to assess the efficacy of the metabolites on the Onchocerca worm. The antifilarial activity was evaluated in vitro on microfilariae (Mfs) and adult worms of the bovine derived Onchocerca ochengi, a close relative of Onchocerca volvulus. Cytotoxicity was assessed in vitro on monkey kidney epithelial cells. The structures of the active compounds were determined using spectroscopic methods and their drug-likeness evaluated using Lipinski parameters. Two secondary metabolites, AMJ1 [containing mustakone (1) as the major component] and linoleic acid or (9Z,12Z)-octadeca-9,12-dienoic acid (2) were isolated. Both compounds were found to kill both the microfilariae and adult worms of O. ochengi in a dose dependent manner. The IC50s for AMJ1 were 15.7 µg/mL for Mfs, 17.4 µg/mL for adult males and 21.9 µg/mL for adult female worms while for linoleic acid the values were, 15.7 µg/mL for Mfs, 31.0 µg/mL for adult males and 44.2 µg/mL for adult females. The present report provides the first ever evidence of the anti-Onchocerca efficacy of AMJ1 and linoleic acid. Thus, these secondary metabolites may provide a lead for design and development of new antifilarial agents.Electronic supplementary materialThe online version of this article (doi:10.1007/s13659-014-0023-5) contains supplementary material, which is available to authorized users.
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