The turnover of soil organic carbon (SOC) in cropland plays an important role in terrestrial carbon cycling, but little is known about the temperature sensitivity (Q 10 ) of SOC decomposition below the topsoil layer of arable soil. Here, samples of topsoil (0-20 cm) and subsoil (20-40 cm) layers were obtained from paddy fields and upland croplands in two regions of China. Using a sequential temperature changing method, soil respiration rates were calculated at different temperatures (8 °C to 28 °C) and fitted to an exponential equation to estimate Q 10 values. The average SOC decomposition rate was 59% to 282% higher in the topsoil than in the subsoil layer because of higher labile carbon levels in the topsoil. However, Q 10 values in the topsoil layer (5.29 ± 1.47) were significantly lower than those in the subsoil layer (7.52 ± 1.84). The pattern of Q 10 values between the topsoil and subsoil was significantly negative to labile carbon content, which is consistent with the carbon quality-temperature hypothesis. These results suggest that the high temperature sensitivity of SOC decomposition in the subsoil layer needs to be considered in soil C models to better predict the responses of agricultural SOC pools to global warming.Soils contain approximately 1,500 Pg organic carbon (C) in the global upper 100 cm, which is about three times the amount stored in terrestrial vegetation (550 Pg) and twice that stored in the atmosphere (750 Pg) 1, 2 . The soil organic carbon (SOC) pool plays important roles in the cycling and balance of global C 3 . The global storage of SOC in cropland is about 128-165 Pg C 4 , which is approximately 8% to 10% of the terrestrial SOC pool 5,6 . In addition to being an important part of global SOC storage, SOC in cropland is the most active SOC pool among terrestrial ecosystems 7 .Global warming has already had observable effects on the environment 8 . Although warming is expected to accelerate SOC decomposition, the responses of SOC to warming still exhibit many uncertainties 3 . One of the key factors leading to these uncertainties is the temperature sensitivity (Q 10 ) of SOC decomposition 3 . SOC storage in cropland is not only influenced by climate change, but is also regulated by human activities over a short period of time. Therefore, understanding the Q 10 of SOC decomposition in cropland is important for understanding global C cycling. Unlike natural soils, cropland soils have relatively low organic C concentrations, and thus a higher C sequestration potential 4 . Sustainable land use and management practices in agroecosystems could have the potential to sequester approximately 55-78 Pg SOC 9 .Many studies have focused on soil respiration in croplands (e.g. Lohila et al. 10 , Fiener et al. 11 , and Campos 12 ). Field-measured soil respiration is the CO 2 flux emitted from the soil surface, which commonly includes both autotrophic and heterotrophic respiration 13 , with autotrophic respiration occupying 30% to 70% of the total 14, 15 . Therefore, field measurements can not clearl...