Manganese-Enhanced Magnetic Resonance Imaging: Application in Central Nervous System Diseases

Yang, Jun; Li, Qinqing

doi:10.3389/fneur.2020.00143

Cited by 14 publications

(14 citation statements)

References 178 publications

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“…MEMRI has been increasingly exploited for identifying neural circuits associated with specific behaviors in similar experimental settings (Eschenko et al, 2010b;Gildish et al, 2012;Bangasser et al, 2013;McGuire et al, 2013;Laine et al, 2017;Yang and Li, 2020).…”

Section: Discussionmentioning

confidence: 99%

Neuroimaging reveals functionally distinct neuronal networks associated with high-level alcohol consumption in two genetic rat models

Pallarés

Dudek

Moreno

et al. 2020

Behavioural Pharmacology

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Human imaging data suggest that the motivational processes associated with alcohol reward are reflected in the patterns of neural activation after alcohol or alcohol-related cues. In animal models of alcohol drinking, however, the changes in brain activation during voluntary alcohol ingestion are poorly known. In order to improve the translational utility of animal models, we examined alcoholinduced functional brain activation in AA (Alko Alcohol) and Marchigian-Sardinian alcohol-preferring (msP) rats that drink voluntarily high levels of alcohol, but exhibit widely different neurochemical and behavioral traits co-segregated with alcohol preference. Brain imaging was performed using manganese-enhanced magnetic resonance imaging (MEMRI), which is based on accumulation of Mn 2+ ions in activated neurons, allowing the identification of functional neuronal networks recruited during specific behaviors in awake animals during a subsequent imaging session under anesthesia.MEMRI was performed following four weeks of voluntary alcohol drinking, using water drinking as the control. Despite similar levels of alcohol drinking, strikingly different alcohol-induced neuronal activity patterns were observed in AA and msP rats. Overall, functional activation in the AA rats was more widespread, involving large cortical areas and subcortical structures, such as the bed nucleus of the stria terminalis, preoptic area, hypothalamus, periaqueductal grey, and substantia nigra. In the msP rats, however, alcohol-related activation was largely confined to prefrontal cortical regions and insular cortex, and olfactory areas. Overlapping areas of activation found in both rat lines included the nucleus accumbens, prelimbic, orbital, and insular cortex. In conclusion, our data reveal strikingly different brain circuits associated with alcohol drinking in two genetically different rat lines and suggest innately different motivational and behavioral processes driving alcohol drinking. These findings have important implications for the use of these lines in translational alcohol research.

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Section: Discussionmentioning

confidence: 99%

Neuroimaging reveals functionally distinct neuronal networks associated with high-level alcohol consumption in two genetic rat models

Pallarés

Dudek

Moreno

et al. 2020

Behavioural Pharmacology

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“…MEMRI is, therefore, an advantageous diagnostic method to visualize the activity and anatomy of those regions of the body in which Mn 2+ accumulates [8]. It is currently mainly used to study cell structure, neuronal connections and brain function [11].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, the neurotoxic effects are the main limiting factor for the use of Mn as a contrast agent in clinical practice. Indeed, MR relaxation times are directly proportional to the tissues concentration of Mn 2+ ions, and the greater the Mn 2+ amount the stronger and detectable the contrast and the better the delineation of anatomical structures [8,11]. Therefore, in order to minimize side effects, the critical point for the use of Mn as a contrast agent is the need to employ the lowest dose sufficient to be detected when using MRI.…”

Section: Introductionmentioning

confidence: 99%

Manganese in Diagnostics: A Preformulatory Study

et al. 2022

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This investigation aims to find lipid-based nanosystems to be used as tools to deliver manganese for diagnostic purposes in multimodal imaging techniques. In particular, the study describes the production and characterization of aqueous dispersions of anionic liposomes as delivery systems for two model manganese-based compounds, namely manganese chloride and manganese acetylacetonate. Negatively charged liposomes were obtained using four different anionic surfactants, namely sodium docusate (SD), N-lauroylsarcosine (NLS), Protelan AG8 (PAG) and sodium lauroyl lactylate (SLL). Liposomes were produced by the direct hydration method followed by extrusion and characterized in terms of size, polydispersity, surface charge and stability over time. After extrusion, liposomes are homogeneous and monodispersed with an average diameter not exceeding 200 nm and a negative surface charge as confirmed by ζ potential measurement. Moreover, as indicated by atomic absorption spectroscopy analyses, the loading of manganese-based compounds was almost quantitative. Liposomes containing NLS or SLL were the most stable over time and the presence of manganese-based compounds did not affect their size distribution. Liposomes containing PAG and SD were instable and therefore discarded. The in vitro cytotoxicity of the selected anionic liposomes was evaluated by MTT assay on human keratinocyte. The obtained results highlighted that the toxicity of the formulations is dose dependent.

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“…MRI relaxation times are directly proportional to the concentration of Mn 2+ ions in the tissues. A higher amount of Mn 2+ results in a stronger and more detectable contrast, leading to better image quality [ 4 ]. Currently, the main limiting factor for the use of Mn 2+ ion as a contrast agent in clinical practice is its neurotoxic effects [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese

et al. 2022

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The development of multimodal imaging techniques such as positron emission tomography (PET) and magnetic resonance imaging (MRI) allows the contemporary obtaining of metabolic and morphological information. To fully exploit the complementarity of the two imaging modalities, the design of probes displaying radioactive and magnetic properties at the same time could be very beneficial. In this regard, transition metals offer appealing options, with manganese representing an ideal candidate. As nanosized imaging probes have demonstrated great value for designing advanced diagnostic/theranostic procedures, this work focuses on the potential of liposomal formulations loaded with a new synthesized paramagnetic Mn(II) chelates. Negatively charged liposomes were produced by thin-layer hydration method and extrusion. The obtained formulations were characterized in terms of size, surface charge, efficiency of encapsulation, stability over time, relaxivity, effective magnetic moment, and in vitro antiproliferative effect on human cells by means of the MTT assay. The negatively charged paramagnetic liposomes were monodisperse, with an average hydrodynamic diameter not exceeding 200 nm, and they displayed good stability and no cytotoxicity. As determined by optical emission spectroscopy, manganese complexes are loaded almost completely on liposomes maintaining their paramagnetic properties.

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Manganese-Enhanced Magnetic Resonance Imaging: Application in Central Nervous System Diseases

Cited by 14 publications

References 178 publications

Neuroimaging reveals functionally distinct neuronal networks associated with high-level alcohol consumption in two genetic rat models

Neuroimaging reveals functionally distinct neuronal networks associated with high-level alcohol consumption in two genetic rat models

Manganese in Diagnostics: A Preformulatory Study

Synthetic and Nanotechnological Approaches for a Diagnostic Use of Manganese

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