Manganese–Iron Dual Single-Atom Catalyst with Enhanced Nanozyme Activity for Wound and Pustule Disinfection

Abstract

Even though great progress has been achieved in mimicking natural enzyme engineering, few artificial enzymes with efficient catalytic performance and multifunction have been reported. In this study, novel manganese–iron dual single-atom catalysts (Mn/Fe SACs) were synthesized via a hydrothermal/pyrolysis recipe. Iron atoms inside the Mn/Fe SACs adequately exerted the peroxidase (POD)-like activity, its Michaelis–Menten constant, and maximum initial velocity superior to the horseradish peroxidase. Manganese atoms sufficiently catalyzed the H2O2 to generate oxygen (O2), which alleviated the challenge of the continued lack of O2 in the infected wound. In addition, Mn/Fe SACs possess a glutathione oxidase-like activity that further enhanced POD-like activity in the therapeutic process. The antibacterial rates of Mn/Fe SACs were 95 and 94.5% for Escherichia coli and Staphylococcus aureus, respectively. In vitro anti-inflammatory experiments demonstrated that Mn/Fe SACs could regulate the polarization of macrophages into the anti-inflammatory M2 subtype. In vivo wound healing experiments suggested that the combination therapy of Mn/Fe SACs and chemodynamic therapy presented a great promotion of the recovery rate. Moreover, the O2 generated by the catalase-like process contributed to the catalysts permeating the interior of the infected wounds and achieved preferable abscess elimination ability. This work revealed the potential of Mn/Fe SACs as broad-spectrum antimicrobial materials, which provided a novel strategy for treating infected and abscess wounds.

Fe-Capsaicin Nanozymes Attenuate Sepsis-Induced Acute Lung Injury via NF-κB Signaling

“… 40 Zhang et al synthesized novel manganese-iron dual single-atom catalysts (Mn/Fe SACs) to promote wound healing by modulating macrophage polarization into the anti-inflammatory M2 type. 41 Among the diverse nanocatalysts, iron-based biocatalysts have received special attention in diagnosis and disease treatment. 42 Studies have shown that iron-based nanozymes can be used to treat Alzheimer’s disease and cardiovascular diseases, and can also act as anti-inflammatory agents to protect cells from ROS damage.…”

Advanced nanozymes possess peroxidase-like catalytic activities in biomedical and antibacterial fields: review and progress

“…However, this enzyme activity has recently been shown to also enhance the antimicrobial effect of POD nanozyme. Zhang et al developed a bimetallic single-atom nanozyme (Mn/Fe SACs) through high-temperature pyrolysis, 59 which simultaneously exhibited POD, CAT, and GSH-oxidase-like activities. Under acidic conditions, these Mn/Fe SACs could perform the POD and GSH-oxidase-like catalytic effect to kill bacteria.…”

Single-Atom-Based Nanoenzyme in Tissue Repair

“…Dual SAzymes are also reported based on the existing SAzyme. − Dual-metal atom nanozymes (DAzymes), including two identical or different metal atoms as active centers, represent a significant advancement in the field of catalysis, offering distinct advantages over their single-atom catalyst counterparts. By incorporating a second metal atom, DAzymes effectively overcome some limitations associated with SACs.…”