Manganese Reduction by Microbes from Oxic Regions of the Lake Vanda (Antarctica) Water Column

Bratina, Bonnie Jo; Stevenson, Bradley S.; Green, William J.; Schmidt, Thomas M.

doi:10.1128/aem.64.10.3791-3797.1998

Cited by 75 publications

(31 citation statements)

References 44 publications

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“…strain MB6-08, a halophile isolated from Antarctic sea ice (Bowman et al, 1997). Despite high concentrations of manganese in Lake Bonney, no sequences were found to be close to the low G+C Mn-reducing Carnobacterium species found in Lake Vanda, or the similar Carnobacterium sequences found in Ace Lake and the accretion ice of Lake Vostok (Franzmann et al, 1991;Bratina et al, 1998;Christner et al, 2001).…”

Section: Gram-positives -Actinobacteria and Firmicutessupporting

confidence: 52%

Planktonic microbial community composition across steep physical/chemical gradients in permanently ice‐covered Lake Bonney, Antarctica

et al. 2006

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Lake Bonney is a chemically stratified, permanently ice‐covered Antarctic lake that is unusual because anomalous nutrient concentrations in the east lobe suggest that denitrification occurs in the deep suboxic waters of the west lobe but not the east lobe, resulting in high concentrations of nitrate and nitrite below the east lobe chemocline. Environmental factors that usually control denitrification rates (e.g. organic carbon, nitrate, oxygen) do not appear to explain the nitrate distribution in the east lobe, suggesting that other factors (e.g. trace metals, salts, microbial community structure, etc.) may be involved. In order to explore the potential importance of microbial community composition, samples collected from multiple depths in both lobes were compared on the basis of 16S rRNA gene diversity. 16S rRNA polymerase chain reaction (PCR) clone libraries generated from five depths were subjected to restriction fragment length polymorphism (RFLP), rarefaction, statistical and phylogenetic analyses. Bacterial and archaeal 16S rRNA gene sequences were determined for clones corresponding to unique RFLP patterns. The bacterial community below the chemocline (at 25 m) in the east lobe was the least diverse of the five depths analysed and was compositionally distinct from the communities of the overlying waters. The greatest compositional overlap was observed between 16 and 19 m in the east lobe, while the east lobe at 25 m and the west lobe at 13 and 16 m had relatively distinct communities. Despite very little compositional overlap between the suboxic, hypersaline depths of the east and west lobes (25 m and 16 m, respectively), sequences closely related to the denitrifying Marinobacter strain ELB17 previously isolated from the east lobe were found in both libraries. Most of the Lake Bonney sequences are fairly distinct from those reported from other Antarctic environments. Archaeal 16S rRNA genes were only successfully amplified from the two hypersaline depths analysed, with only one identical halophilic sequence type occurring in both libraries, indicating extremely low archaeal diversity. Overall, microbial community composition varies both between lobes and across depths within lobes in Lake Bonney, reflecting the steep gradients in physical/chemical parameters across the chemocline, as well as the anomalous nutrient chemistry of the system.

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Section: Gram-positives -Actinobacteria and Firmicutessupporting

confidence: 52%

Planktonic microbial community composition across steep physical/chemical gradients in permanently ice‐covered Lake Bonney, Antarctica

et al. 2006

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“…2) were 98-100% similar to 16S rRNA gene sequences of known manganese-reducing bacteria isolated from an extremely oligotrophic environment in permanently ice-covered, stratified Antarctic lake Vanda (Carnobacterium alterfundicum) [48]. They were also related to isolate LV62:W1, a Carnobacterium species isolated from the same area [6]. Sequence type G (Fig.…”

Section: Phylogenetic Affiliations Of Predominant Community Membersmentioning

confidence: 64%

Molecular monitoring of culturable bacteria from deep-sea sediment of the Nankai Trough, Leg 190 Ocean Drilling Program

Toffin

Webster

Weightman

et al. 2004

FEMS Microbiology Ecology

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Culturable bacteria were detected in deep-sea sediment samples collected from the Nankai Trough site 1173 (Ocean Drilling Program, ODP, Leg 190) at 4.15 m below the seafloor with 4791 m of overlying water. In this deep ocean near surface sediment, mainly fermentative heterotrophs, autotrophic acetogens and sulfate-reducing bacteria were enriched by using two different non-selective enrichment culture media. Culturable bacterial population shifts within the deep marine sediment enrichments were monitored by using denaturating gradient gel electrophoresis (DGGE). DGGE analysis revealed a decrease in the number of 16S rRNA gene fragments from high to low carbon concentrations, and from low to high dilution of inoculum, suggesting that fast-growing bacteria were numerically dominant in enrichment culture samples. The dominant 16S rRNA fragments observed in DGGE gels were assigned to the Firmicutes, Proteobacteria (gamma and delta subgroups) and Spirochaeta phyla. Continual sub-culture and purification resulted in two isolates which were phylogenetically identified as members of the genera Acetobacterium and Marinilactibacillus. Our results, which combine enrichment culturing with DGGE analysis, indicated that enrichment cultures derived from inoculum dilution and media with various concentrations of carbon could facilitate the detection and isolation of a greater number of environmentally relevant bacterial species than when using traditional enrichment techniques alone.

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“…The results obtained argue that representatives of at least five bacterial lineages are likely to be present in Lake Vostok, some of which are related, in terms of 16S rDNA sequences, to isolates from other cold, potentially very similar environments. For example, sequence pD12 is < 92% identical to 16S rDNA sequences from two Carnobacterium species that were isolated from icecovered Antarctic lakes (Franzmann et al, 1991;Bratina et al, 1998), and sequence pA419 clusters with both the 16S rDNA sequence of an isolate recovered from 400 mbs of Lake Baikal in Siberia and the sequence of an amplicon from a frozen Antarctic lake (Fig. 3).…”

Section: Discussionmentioning

confidence: 99%

Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice

Christner

Mosley‐Thompson

Thompson

et al. 2001

Environmental Microbiology

248

150

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Lake Vostok, the largest subglacial lake in Antarctica, is separated from the surface by approximately 4 km of glacial ice. It has been isolated from direct surface input for at least 420 000 years, and the possibility of a novel environment and ecosystem therefore exists. Lake Vostok water has not been sampled, but an ice core has been recovered that extends into the ice accreted below glacial ice by freezing of Lake Vostok water. Here, we report the recovery of bacterial isolates belonging to the Brachybacteria, Methylobacterium, Paenibacillus and Sphingomonas lineages from a sample of melt water from this accretion ice that originated 3593 m below the surface. We have also amplified small-subunit ribosomal RNA-encoding DNA molecules (16S rDNAs) directly from this melt water that originated from alpha- and beta-proteobacteria, low- and high-G+C Gram-positive bacteria and a member of the Cytophaga/Flavobacterium/Bacteroides lineage.

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Manganese Reduction by Microbes from Oxic Regions of the Lake Vanda (Antarctica) Water Column

Cited by 75 publications

References 44 publications

Planktonic microbial community composition across steep physical/chemical gradients in permanently ice‐covered Lake Bonney, Antarctica

Planktonic microbial community composition across steep physical/chemical gradients in permanently ice‐covered Lake Bonney, Antarctica

Molecular monitoring of culturable bacteria from deep-sea sediment of the Nankai Trough, Leg 190 Ocean Drilling Program

Isolation of bacteria and 16S rDNAs from Lake Vostok accretion ice

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