Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts

Oberley, Larry W.; McCormick, Michael L.; Sierra-Rivera, Elaine; Clair, Daret K. St.

doi:10.1016/0891-5849(89)90083-x

Cited by 75 publications

(34 citation statements)

References 7 publications

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“…Cells were harvested under 4 different sets of growth conditions: exponential fed, exponential unfed, confluent fed, and confluent unfed. In contrast to our previous work with human embryonic lung fibroblasts (Oberley et al, 1989), we were unable to discern any differences in anti-oxidant enzyme activities among the various groups (data not shown). Therefore, the results from all growth conditions were averaged.…”

Section: Resultscontrasting

confidence: 86%

Antioxidant enzyme activities in normal and transformed mouse liver cells

Sun

Oberley

Elwell

et al. 1989

Intl Journal of Cancer

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101

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Copper- and zinc-containing superoxide dismutase (CuZnSOD), manganese-containing superoxide dismutase (MnSOD), catalase (CAT), glutathione peroxidase (GPX, both Se-dependent and Se-independent), and glutathione reductase (GR) were measured in normal, nitrosoguanidine-transformed and SV40-transformed mouse liver cells in culture, as well as in mouse liver homogenates. Enzyme activities were compared on the basis of 3 different endpoints: per mg protein, per mg DNA, and per 10(6) cells. Except for GR, activity of all the measured anti-oxidant enzymes was much higher in vivo than in vitro. All of the anti-oxidant enzyme activities were lower in general in the 2 transformed cell lines than in the in vitro normal cell line, except Cu-ZnSOD, which showed little change. However, MnSOD was the only enzyme which showed lowered activity in both transformed cell lines, no matter what endpoint was used. This finding is in agreement with previous work showing lowered MnSOD activity in tumor cells.

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Section: Resultscontrasting

confidence: 86%

Antioxidant enzyme activities in normal and transformed mouse liver cells

Sun

Oberley

Elwell

et al. 1989

Intl Journal of Cancer

Self Cite

101

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“…MnSOD protein levels and activity were found to be higher in quiescent (G 0 ) compared with proliferating NIH 3T3 mouse fibroblasts and WI38 human lung fibroblasts (40,56,60). NIH 3T3 fibroblasts synchronized to quiescence by serum starvation showed a significant increase in MnSOD expression, which decreased substantially during S phase after the synchronized cells had been allowed to enter the proliferative cycle by adding serum to the culture medium (40).…”

Section: Sarsour Et Almentioning

confidence: 97%

Manganese Superoxide Dismutase Regulates a Redox Cycle Within the Cell Cycle

Sarsour

Kalen²,

Goswami³

2014

Antioxidants & Redox Signaling

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Significance: Manganese superoxide dismutase (MnSOD) is a nuclear-encoded and mitochondria-matrixlocalized oxidation-reduction (redox) enzyme that regulates cellular redox homeostasis. Cellular redox processes are known to regulate proliferative and quiescent growth states. Therefore, MnSOD and mitochondria-generated reactive oxygen species (ROS) are believed to be critical regulators of quiescent cells' entry into the cell cycle and exit from the proliferative cycle back to the quiescent state. Recent Advances/Critical Issues: Recent evidence suggests that the intracellular redox environment fluctuates during the cell cycle, shifting toward a more oxidized status during mitosis. MnSOD activity is higher in G 0 /G 1 cells compared with S, G 2 and M phases. After cell division, MnSOD activity increases in the G 1 phase of the daughter generation. The periodic fluctuation in MnSOD activity during the cell cycle inversely correlates with cellular superoxide levels as well as glucose and oxygen consumption. Based on an inverse correlation between MnSOD activity and glucose consumption during the cell cycle, it is proposed that MnSOD is a central molecular player for the ''Warburg effect.'' Future Directions: In general, loss of MnSOD activity results in aberrant proliferation. A better understanding of the MnSOD and mitochondrial ROS-dependent cell cycle processes may lead to novel approaches to overcome aberrant proliferation. Since ROS have both deleterious (pathological) and beneficial (physiological) effects, it is proposed that ''eustress'' should be used when discussing ROS processes that regulate normal physiological functions, while ''oxidative stress'' should be used to discuss the deleterious effects of ROS.

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“…Antimouse Thy 1.2 mAb from Sigma Chemical Co. (St. Louis, MO) and horseradish peroxidase-conjugated avidin from Bio-Rad Laboratories (Hercules, CA) were used. Polyclonal rabbit anti-hMnSOD Ab [43] was provided by Dr. L. Oberley (Iowa City, IA). Baby rabbit complement was purchased from Pel-Freez Clinical Systems (Brown Deer, WI).…”

Section: Ab and Other Reagentsmentioning

confidence: 99%

Production of reactive oxygen intermediates following CD40 ligation correlates with c-Jun N-terminal kinase activation and IL-6 secretion in murine B lymphocytes

Lee

Koretzky

1998

Eur. J. Immunol.

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Recent studies indicate that reactive oxygen intermediates (ROI) serve as second messengers in cell signaling. ROI have been implicated in the activation of NF-kappaB as well as c-Jun N-terminal kinase (JNK) in response to IL-1 and TNF-alpha stimulation. In this report we examine whether intracellular ROI are involved in CD40 receptor signaling. We show that CD40 engagement on resting splenic B lymphocytes and murine B lymphoma WEHI 231 cells generates ROI. Blocking ROI production by preincubation with the antioxidant N-acetyl-L-cysteine inhibits JNK activation, NF-kappaB-driven luciferase activity, and IL-6 secretion following CD40 ligation, suggesting a role for ROI in CD40-mediated signaling events. Furthermore, transfection of WEHI 231 cells with a plasmid encoding Mn-superoxide dismutase interferes with CD40-induced NF-kappaB activation, providing further support for ROI involvement in this pathway. Collectively, these data demonstrate that ROI may serve as second messengers linking CD40 engagement on B cells to important downstream activation events.

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Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts

Cited by 75 publications

References 7 publications

Antioxidant enzyme activities in normal and transformed mouse liver cells

Antioxidant enzyme activities in normal and transformed mouse liver cells

Manganese Superoxide Dismutase Regulates a Redox Cycle Within the Cell Cycle

Production of reactive oxygen intermediates following CD40 ligation correlates with c-Jun N-terminal kinase activation and IL-6 secretion in murine B lymphocytes

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