Mangiferin inhibits lipopolysaccharide‐induced epithelial‐mesenchymal transition (<scp>EMT</scp>) and enhances the expression of tumor suppressor gene <i>PER1</i> in non‐small cell lung cancer cells

Lin, Yen Sung; Tsai, Kun Ling; Chen, Jia Ni; Wu, C.

doi:10.1002/tox.22943

Cited by 22 publications

(24 citation statements)

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“…As a circadian rhythm gene, PER1 has an important role in the cell cycle and affects the occurrence of cancer, including colorectal cancer (Holipah and Kuroda, 2020). In non-smallcell lung cancer, mangiferin inhibits lipopolysaccharide-induced epithelial-mesenchymal transition and enhances the expression of PER1 (Lin et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have shown that PER1 directly regulates levels of reactive oxygen species in multiple organs and regulates key effectors of energy substrate utilization in response to daily behavioral rhythms and oxidative stress, thereby maintaining the daily rhythms of mitochondrial morphology and function (Sun et al, 2020). Changes in expression levels of PER1 have been found in some cancers, including pancreatic cancer (Guo et al, 2020), oral squamous cell carcinoma (Yang et al, 2020), endometrial cancer (Wang et al, 2020), colorectal cancer (Holipah and Kuroda, 2020), and non-small-cell carcinoma (Lin et al, 2020), but this has not been studied in OV. It has also been reported that the occurrence and development of cancer, as well as its prognosis and treatment outcomes, are closely related to the abnormal expression of certain circadian clock genes (Cao et al, 2009;Sahar and Sassone-Corsi, 2009;Yang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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PER1 Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Ovarian Cancer

et al. 2021

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Background: Period circadian protein homolog 1 (PER1) is an important component of the biorhythm molecular oscillation system and plays an important part in the development and progression of mammalian cancer. However, the correlations of PER1 with prognosis and tumor-infiltrating lymphocytes in ovarian cancer (OV) remain unclear.Methods: The Oncomine and TIMER databases were used to examine the expression of PER1 in OV. Kaplan–Meier Plotter and PrognoScan were used to evaluate the relationship between PER1 and prognosis. Kaplan–Meier Plotter was used to analyze the relationships between PER1 and clinicopathological features of OV patients. The relationship between PER1 expression and immune infiltration in OV was investigated using the TIMER database and CIBERSORT algorithm. The STRING database was used to analyze PER1-related protein functional groups, the GeneMANIA online tool was used to analyze gene groups with similar functions to those of PER1, and Network Analyst was used to identify transcription factors that regulate PER1. The correlation between PER1 and immunoinvasion of OV was analyzed using TIMER. Finally, quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect PER1 expression.Results: PER1 was differentially expressed in different cancer tissues, and its expression in various OV subtypes was lower than that in normal ovarian tissue. OV patients with low PER1 expression had a reduced overall survival rate. Decreased PER1 expression in stage 1 and stage 1+2 OV patients was related to poor prognosis, while increased PER1 expression in stage 3+4 patients and TP53 mutation were related to poor overall survival and progression-free survival. We identified eight genes whose expression was strongly correlated with that of PER1, as well as four transcription factors that regulate PER1. In OV, PER1 expression levels were positively correlated with infiltration levels of cells including neutrophils, regulatory T cells, and M2 macrophages, and closely related to a variety of immune markers. Reduced expression of PER1 was significantly associated with poor overall survival.Conclusion: These findings suggest that PER1 could be used as a prognostic biomarker to determine prognosis and immune infiltration in OV patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PER1 Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Ovarian Cancer

et al. 2021

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“…For this reason, it can be adapted to various types of cell lines and has been extensively used in NSCLC cells (11,(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40), where it has proven to be very useful. In the transwell assay, cells migrate vertically through the membrane from the upper chamber to the lower.…”

Section: Migration Assaysmentioning

confidence: 99%

“…It also reduced the secretion of IL-1β, up-regulated E-cadherin, and downregulated vimentin. Importantly, mangiferin suppressed migration by decreasing the expression of C-X-C chemokine receptor type 4 (CXCR4) (30).…”

Section: Polyphenols Non-flavonoidsmentioning

confidence: 99%

A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies

Albuquerque¹,

Manguinhas²,

Costa³

et al. 2021

Transl Lung Cancer Res

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Lung cancer (LC) is the leading cause of cancer deaths worldwide, being non-small lung cancer (NSCLC) sub-types the most prevalent. Since most LC cases are only detected during the last stage of the disease the high mortality rate is strongly associated with metastases. For this reason, the migratory and invasive capacity of these cancer cells as well as the mechanisms involved have long been studied to uncover novel strategies to prevent metastases and improve the patients' prognosis. This narrative review provides an overview of the main in vitro migration and invasion assays employed in NSCLC research. While several methods have been developed, experiments using conventional cell culture models prevailed, specifically the wound-healing and the transwell migration and invasion assays. Moreover, it is provided herewith a summary of the available information concerning chemical contaminants that may promote the migratory/ invasive properties of NSCLC cells in vitro, shedding some light on possible LC risk factors. Most of the reported agents with pro-migration/invasion effects derive from cigarette smoking [e.g., Benzo(a)pyrene and cadmium] and air pollution. This review further presents several studies in which different dietary/ plant-derived compounds demonstrated to impair migration/invasion processes in NSCLC cells in vitro.These chemicals that have been proposed as anti-migratory consisted mainly of natural bioactive substances, including polyphenols non-flavonoids, flavonoids, bibenzyls, terpenes, alkaloids, and steroids. Some of these compounds may eventually represent novel therapeutic strategies to be considered in the future to prevent metastasis formation in LC, which highlights the need for additional in vitro methodologies that more closely resemble the in vivo tumor microenvironment and cancer cell interactions. These studies along with adequate in vivo models should be further explored as proof of concept for the most promising compounds.

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“…One in vitro experiment showed that mangiferin exerts an influence on cell cycle arrest and induces apoptosis in A549 cells [12]. In addition, several studies have suggested that mangiferin plays a reverse or inhibitory role in lung carcinogenesis [13,14]. However, the precise molecular mechanism remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Mangiferin Inhibits Human Lung Adenocarcinoma by Suppressing MiR-27b and MiR-92a

Chi

Meng

Chen

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Lung adenocarcinoma (LUAD) is one of the most prevalent malignancies. However, its mechanism and therapeutic strategy remain to be clarified. Mangiferin is a flavonoid derived from the leaves of mango trees of the lacquer family that has many pharmacological and physiological effects. This research aimed to elucidate the biological effect of mangiferin in LUAD cell lines and clarify the in vitro mechanism of mangiferin. Mangiferin was shown to significantly restrain the proliferation of LUAD cells (A549, H1299, and H2030 cells) in a dose- and time-dependent manner. Furthermore, mangiferin was capable of stimulating apoptosis, and more cells were blocked in G1 and S phase in the mangiferin-treated cells than in those not treated with mangiferin. Microarrays and micro-RNA sequencing data suggested that there is a higher level of miR-27b and miR-92a in LUAD tissues than in non-LUAD tissues. Additional experiments indicated that mangiferin may be related to the downregulated levels of miR-92a and miR-27b. In conclusion, mangiferin likely regulates proliferation and apoptosis in LUAD cells by reducing the expression levels of miR-92a and miR-27b.

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Mangiferin inhibits lipopolysaccharide‐induced epithelial‐mesenchymal transition (EMT) and enhances the expression of tumor suppressor gene PER1 in non‐small cell lung cancer cells

Cited by 22 publications

References 48 publications

PER1 Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Ovarian Cancer

PER1 Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Ovarian Cancer

A narrative review of the migration and invasion features of non-small cell lung cancer cells upon xenobiotic exposure: insights from in vitro studies

Mangiferin Inhibits Human Lung Adenocarcinoma by Suppressing MiR-27b and MiR-92a

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