Mangotoxin: a novel antimetabolite toxin produced by Pseudomonas syringae inhibiting ornithine/arginine biosynthesis

Arrebola, Eva; Cazorla, Francisco M.; Durán, Victoria E; Rivera, Eugenia; Olea, Francisco; Codina, Juan; Pérez-Garcı́a, Alejandro; Vicente, Antonio de

doi:10.1016/j.pmpp.2003.11.003

Cited by 53 publications

(82 citation statements)

References 40 publications

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“…Three pathovar tomato strains produced an unnamed antimetabolite toxin ( Fig. 1) that inhibits the conversion of N-acetyl-L-glutamate to N-acetyl-ornithine (11,51). Additionally, strain P. syringae pv.…”

Section: Resultsmentioning

confidence: 99%

“…syringae and to contribute to its virulence in tomato plants (8,11). Recently, Murillo et al (13) reported the production of mangotoxin by strains of pathovar avellanae, as well as the simultaneous production of two antimetabolite toxins, mangotoxin and phaseolotoxin, by strain P. syringae pv.…”

Section: Discussionmentioning

confidence: 99%

“…syringae produces several type III effectors and virulence factors, but the role of its toxins is particularly significant during symptom development (1,(8)(9)(10). The current study is focused on mangotoxin, which inhibits the enzyme ornithine N-acetyltransferase (11,12). Mangotoxin was initially detected in strains from P. syringae pv.…”

mentioning

confidence: 99%

“…Mangotoxin was initially detected in strains from P. syringae pv. syringae (11); however, its production was recently observed in strains of pathovar avellanae (13). Recent studies have reported the involvement of the mgo (8,12,14) and mbo (15) operons in mangotoxin production and P. syringae pv.…”

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confidence: 99%

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The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

Carrión

Gutiérrez‐Barranquero

Arrebola

et al. 2013

Appl Environ Microbiol

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c Mangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplified a 692-bp DNA fragment from 52 mangotoxin-producing strains and from 7 non-mangotoxin-producing strains that harbor the mbo operon, whereas 35 non-mangotoxin-producing strains did not yield any amplification. This, together with the analysis of draft genomes, allowed the identification of the mbo operon in five pathovars (pathovars aptata, avellanae, japonica, pisi, and syringae), all of which belong to genomospecies 1, suggesting a limited distribution of the mbo genes in the P. syringae complex. Phylogenetic analyses using partial sequences from housekeeping genes differentiated three groups within genomospecies 1. All of the strains containing the mbo operon clustered in groups I and II, whereas those lacking the operon clustered in group III; however, the relative branching order of these three groups is dependent on the genes used to construct the phylogeny. The mbo operon maintains synteny and is inserted in the same genomic location, with high sequence conservation around the insertion point, for all the strains in groups I and II. These data support the idea that the mbo operon was acquired horizontally and only once by the ancestor of groups I and II from genomospecies 1 within the P. syringae complex.T he bacterial plant pathogen Pseudomonas syringae is ubiquitous in nature and often causes economically important plant diseases. This bacterial species establishes an epiphytic population in association with a plant host's surfaces prior to infection. There are at least 50 pathovars, many of which cause a wide range of plant diseases that exhibit diverse symptoms, such as leaf or fruit lesions, cankers, blasts, and galls (1-7).P. syringae produces several type III effectors and virulence factors, but the role of its toxins is particularly significant during symptom development (1,(8)(9)(10). The current study is focused on mangotoxin, which inhibits the enzyme ornithine N-acetyltransferase (11, 12). Mangotoxin was initially detected in strains from P. syringae pv. syringae (11); however, its production was recently observed in strains of pathovar avellanae (13). Recent studies have reported the involvement of the mgo (8,12,14) and mbo (15) operons in mangotoxin production and P. syringae pv. syringae virulence. The mbo operon is composed of six genes, and all of them are directly involved in mangotoxin production.The development of specific methods for pathogen detection in planta is very important. In this sense, different PCR methods have been developed and improved to detect different P. syringae pathovars. For example, one PCR method used primers that were based on the 16S-23S ribosomal genes to identify strains of P. syring...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

Carrión

Gutiérrez‐Barranquero

Arrebola

et al. 2013

Appl Environ Microbiol

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“…The phaseolotoxin is a tripeptide which is hydrolyzed to produce an octicidine metabolite that is an irreversible inhibitor of ornithine carbonoyl transferase (OCTase) (Arrebola et al, 2003;Melotto and Kunkel, 2013). The OCTase enzyme is considered to play a major role in the urea cycle of the plant (Arrebola et al, 2003).…”

Section: Phaseolotoxinsmentioning

confidence: 99%

Virulence strategies of phytopathogenic bacteria and their role in plant disease pathogenesis

Benali¹,

Bencheikh²,

Eddine³

2014

Afr. J. Microbiol. Res.

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Phytopathogenic bacteria have evolved several virulence strategies to face hostile environment of the host plant. In this article, we reviewed the recent progress in research on characterization of the virulence factors including secretion system with their protein effectors, toxins production, extracellular polysaccharides, growth regulators, cell wall degrading enzymes, biofilm formation, siderophores and their role in the plant infection and symptom development focusing particularly on a group of bacteria such as Erwinia amylovora, Agrobacterium tumefaciens, Pseudomonas syringae, Ralstonia solanacearum and Xanthomonas compestris that cause different plant diseases including wilts, spots, blights and cankers. The elucidation of each step in pathogenesis may constitute a key step in any design of new molecules for targeting plant pathogenic bacteria for plant disease control.

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3‐Methylarginine from Pseudomonas syringae pv. syringae 22d/93 Suppresses the Bacterial Blight Caused by Its Close Relative Pseudomonas syringae pv. glycinea

2008

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The epiphyte Pseudomonas syringae pv. syringae 22d/93 (Pss22d) produces a toxin that strongly inhibits the growth of its relative, the plant pathogen P. syringae pv. glycinea. The inhibition can be overcome by supplementing the growth medium with the essential amino acid, L-arginine; this suggests that the toxin acts as an inhibitor of the arginine biosynthesis. The highly polar toxin was purified by bioassay-guided fractionation using ion-exchange chromatography and subsequent RP-HPLC fractionation. The structure of the natural product was identified by HR-ESI-MS, HR-ESI-MS/MS, and NMR spectroscopy experiments as 3-methylarginine. This amino acid has previously only been known in nature as a constituent of the peptide lavendomycin from Streptomyces lavendulae. Results of experiments in which labeled methionine was fed to Pss22d indicated that the key step in the biosynthesis of 3-methylarginine is the introduction of the methyl group by a S-adenosylmethionine (SAM)-dependent methyltransferase. Transposon mutagenesis of Pss22d allowed the responsible SAM-dependent methyltransferase of the 3-methylarginine biosynthesis to be identified.

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Mangotoxin: a novel antimetabolite toxin produced by Pseudomonas syringae inhibiting ornithine/arginine biosynthesis

Cited by 53 publications

References 40 publications

The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

Virulence strategies of phytopathogenic bacteria and their role in plant disease pathogenesis

3‐Methylarginine from Pseudomonas syringae pv. syringae 22d/93 Suppresses the Bacterial Blight Caused by Its Close Relative Pseudomonas syringae pv. glycinea

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