Manifestation of atopic dermatitis‐like skin in <scp>TNCB</scp>‐induced <scp>NC</scp>/Nga mice is ameliorated by topical treatment of substance P, possibly through blockade of allergic inflammation

Choi, Han‐Seok; Kim, Dong-Jin; Nam, Sung-Il; Lim, Sunki; Hwang, Jae Ha; Park, Ki‐Sook; Hong, Hoon Pyo; Shin, Min Kyung; Chung, Eun-Kyung; Son, Youngsook

doi:10.1111/exd.13421

Cited by 11 publications

(8 citation statements)

References 45 publications

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“…The ability of substance P to induce histamine release seems to appear only at high concentrations (Weidner et al, 2000). In line with this, substance P decreases mast cell recruitment and degranulation when used as a topical treatment in a murine model of atopic dermatitis (Choi et al, 2018), pointing to a concentration-dependent dual role of substance P.…”

Section: Itch-related Factors Released By Mast Cellsmentioning

confidence: 64%

Mast Cells and Sensory Nerves Contribute to Neurogenic Inflammation and Pruritus in Chronic Skin Inflammation

Siiskonen

Harvima

2019

Front. Cell. Neurosci.

126

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The intimate interaction between mast cells and sensory nerves can be illustrated by the wheal and surrounding flare in an urticarial reaction in human skin. This reaction is typically associated with an intense itch at the reaction site. Upon activation, cutaneous mast cells release powerful mediators, such as histamine, tryptase, cytokines, and growth factors that can directly stimulate corresponding receptors on itch-mediating sensory nerves. These include, e.g., H1-and H4-receptors, proteaseactivated receptor-2, IL-31 receptor, and the high-affinity receptor of nerve growth factor (TrkA). On the other hand, sensory nerves can release neuropeptides, including substance P and vasoactive intestinal peptide, that are able to stimulate mast cells to release mediators leading to potentiation of the reciprocal interaction, inflammation, and itch. Even though mast cells are well recognized for their role in allergic skin whealing and urticaria, increasing evidence supports the reciprocal function between mast cells and sensory nerves in neurogenic inflammation in chronic skin diseases, such as psoriasis and atopic dermatitis, which are often characterized by distressing itch, and exacerbated by psychological stress. Increased morphological contacts between mast cells and sensory nerves in the lesional skin in psoriasis and atopic dermatitis as well as experimental models in mice and rats support the essential role for mast cellsensory nerve communication in consequent pruritus. Therefore, we summarize here the present literature pointing to a close association between mast cells and sensory nerves in pruritic skin diseases as well as review the essential supporting findings on pruritic models in mice and rats.

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Section: Itch-related Factors Released By Mast Cellsmentioning

confidence: 64%

Mast Cells and Sensory Nerves Contribute to Neurogenic Inflammation and Pruritus in Chronic Skin Inflammation

Siiskonen

Harvima

2019

Front. Cell. Neurosci.

126

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“…The AD-like symptoms of skin induced by DNFB were scored upon completion of drug treatment. The back lesions were evaluated based on clinical presentation criteria ( Choi et al, 2018 ): erythema, edema/papules, epidermal stripping/scratches, and scales (dry), which were categorized into four grades: no lesion, light, medium, and severe (recorded as 0, 1, 2, and 3, respectively). Ear thickness was measured using a Vernier caliper, and the difference between the weight of the left and right ears was calculated as the degree of swelling.…”

Section: Methodsmentioning

confidence: 99%

Study on the Molecular Basis of Huanglian Jiedu Decoction Against Atopic Dermatitis Integrating Chemistry, Biochemistry, and Metabolomics Strategies

Jing

Chen

et al. 2021

Front. Pharmacol.

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Atopic dermatitis (AD) is a common chronic relapsing skin inflammation, which severely affect the quality of life of patients. Inhibiting itching and enhancing immunity to mitigate scratching are key elements in the fight against AD. Huanglian Jiedu decoction (HLJDD) has multiple pharmacological effects in the treatment of AD. However, the effective ingredients and underlying molecular mechanisms have not yet been fully explored. Thus, this study integrates chemistry, biochemistry, and metabolomics strategies to evaluate the active substance basis of HLJDD against AD. First, HLJDD was split to five fractions (CPF, 40AEF, 90AEF, PEF and WEF) and 72 chemical components were identified. NSD (Non-similarity degree) among the different fractions showed significant chemical differences (>81%). Interleukin IL-13, IL-17A, IL-3, IL-31, IL-33, IL4, IL-5, TSLP, IgE, and histamine in the serum, and IL-4Rα, JAK1, and HRH4 levels in skin, participating in inhibiting itching and regulating immunity signaling, were found to be restored to varying degrees in AD treating with HLJDD and its fractions, especially 40AEF and CPF. Untargeted metabolomics analysis demonstrated that forty metabolites were differential metabolites in plasma between the HLJDD-treated group and the AD group, involving in histidine metabolism, arginine biosynthesis, pyrimidine metabolism, and so on. Further, targeted metabolomics analysis revealed that eleven differential metabolites, associating with physiological and biochemical indices, were significant improved in the HLJDD and its fractions groups. In conclusion, HLJDD exhibited anti-AD effects by inhibiting itching and enhancing immunity, which in turn regulating the levels of relative metabolites, and CPF and 40AEF were considered the most important components of HLJDD.

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“…The relative AD severity was evaluated macroscopically on the basis of the following five symptoms: erythema, edema, erosion, dryness, and lichenification [49][50][51]. The total dermatitis severity score was defined as the sum of component scores (0, no symptoms; 1, mild; 2, moderate; 3, severe), ranging from 0 to 15.…”

Section: Evaluation Of Ad-like Skin Symptomsmentioning

confidence: 99%

“…The dermatitis severity was determined on the basis of the sum of the score for each symptom once a week for 3 weeks. In AD-induced mice, skin symptoms such as erythema, edema, erosion, dryness, and lichenification were observed [50,51]. After 3 weeks, topical Biostir-AD-administered mice exhibited all typical symptoms of AD, with a maximum dermatitis score of 7.2 ± 2.2.…”

Section: Effect Of Ut On Ad Symptoms On Mouse Skinmentioning

confidence: 99%

Inhibitory Effects of Urtica thunbergiana Ethanol Extract on Atopic Dermatitis-Induced NC/Nga Mice

et al. 2020

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Atopic dermatitis (AD) is a chronic, inflammatory skin disease that persists or repeatedly recurs in both childhood and adulthood. Urtica thunbergiana (UT) is an aroma herb with little-known pharmacological effects and anti-inflammatory activities against AD. This study investigated the immunomodulatory efficacy of 50% ethanol-extracted UT in necrosis factor-alpha/interferon-gamma (TNF-α/IFN-γ)-stimulated HaCaT cells in vitro and AD-Biostir-induced NC/Nga mice in vivo. The results showed that UT exhibits a dose-dependent increase in scavenged free radicals, reaching 76.0% ± 1.4% of scavenged 1,1-diphenyl-2-picrylhydrazyl at a concentration of 250 µg/mL. In addition, UT significantly downregulated the mRNA expression of the following pro-inflammatory cytokines and chemokines in TNF-α/IFN-γ-stimulated HaCaT cells: interleukin (IL)-6, IL-8, thymus- and activation-regulated chemokine, macrophage-derived chemokine, and regulated on activation normal T expressed and secreted. UT-treated HaCaT cells showed inhibition of the overexpression of chemokine-regulated signaling molecules, such as nuclear factor-kappa B, inhibitor of kappa B (IκBα), signal transducer and activator of transcription 1, and mitogen-activated protein kinases (MAPKs). UT dietary administration in AD-Biostir-induced NC/Nga mice treated and improved AD-like symptoms, such as scales, epidermal thickening, the dermatitis severity score, high trans-epidermal water loss, reduced skin hydration, increased mast cells, elevated serum immunoglobulin E levels, and an enlarged spleen. UT treatment inhibited the expression of phosphorylated forms of MAPKs, nuclear factor of activated T-cells 1, and regulator IκBα. It also upregulated filaggrin (FLG) production. Therefore, UT shows high anti-AD activity both in vitro and in vivo, and can be a useful anti-AD agent.

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Manifestation of atopic dermatitis‐like skin in TNCB‐induced NC/Nga mice is ameliorated by topical treatment of substance P, possibly through blockade of allergic inflammation

Cited by 11 publications

References 45 publications

Mast Cells and Sensory Nerves Contribute to Neurogenic Inflammation and Pruritus in Chronic Skin Inflammation

Mast Cells and Sensory Nerves Contribute to Neurogenic Inflammation and Pruritus in Chronic Skin Inflammation

Study on the Molecular Basis of Huanglian Jiedu Decoction Against Atopic Dermatitis Integrating Chemistry, Biochemistry, and Metabolomics Strategies

Inhibitory Effects of Urtica thunbergiana Ethanol Extract on Atopic Dermatitis-Induced NC/Nga Mice

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