Manifesting viscosity changes in lipid droplets during iodined CT contrast media treatment by the real-time and in situ fluorescence imaging

Mechanistic analysis of viscosity-sensitive fluorescent probes for applications in diabetes detection

“…117 In another attempt by Jin Zhao's group, a novel viscosity sensitive probe based on 3-bromonitrostyryl derivative combined with dicyanomethylene group was introduced. 118 This probe was found to be highly responsive to the changes in lipid droplet viscosity brought about by the adverse side-effects of radiocontrast imaging using an iodinated agent (iodinated contrast media).…”

A triphenylamine-based fluorescent probe with large Stokes shift for wash-free imaging of lipid droplets and diagnosis of fatty liver

“…Compared to conventional detection methods, fluorescence imaging technology stands out owing to its exceptional in situ imaging capability, real-time dynamic monitoring, non-invasiveness, heightened sensitivity and easy operation. 19–21 A considerable array of fluorescent probes has been developed thus far for the purpose of detecting aberrant alterations in LDs, 22–26 but most of them still suffer from a limited Stokes shift, which results in significant background interference and pronounced fluorescence quenching phenomena. These limitations greatly impeded their practical utility in biological imaging.…”

A Lipid Droplet-Specific NIR Fluorescent Probe with a Large Stokes Shift for In Vivo Visualization of Polarity in Contrast-Induced Acute Kidney Injury

“…Fluorescence imaging is a noninvasive, highly sensitive, superior spatiotemporal, and promising method for the in situ and real-time detection of various bioactive molecules in living systems to perceive the onset and progress of diseases at subcellular and in vivo levels. − To date, albeit dozens of fluorescent probes have been devised for specific monitoring and imaging LDs, subtle polarity-sensitive and near-infrared (NIR, >650 nm) fluorescent probes with a larger Stokes shift (>50 nm) are still scarce, which impedes our insight into the intrinsic relationship between LD polarity and disease. NIR emission has been a research focus in the field of fluorescent probe design due to their excellent properties of high tissue/ in vivo penetration capability, remarkable antibackground interference, and low phototoxicity. − In addition, a larger Stokes shift on fluorescent probes is a desirable characteristic, which can greatly reduce self-absorption and minimize the background fluorescence .…”