Manipulating MiRNA Expression: a Novel Approach for Colon Cancer Prevention and Chemotherapy

Ramalingam, Satish; Subramaniam, Dharmalingam; Anant, Shrikant

doi:10.1007/s40495-015-0020-3

Cited by 24 publications

(21 citation statements)

References 151 publications

(145 reference statements)

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“…Explanations could include changes in gene expression in cultured DRG neurons (leading to changes in miRNA target acquisition), and/or increased activities on secondary targets [46] due to overexpression of miRNAs. It is also worth noting that vector-based overexpression of miRNAs dissociates their expression from their normal complex feedback and feedforward regulatory loops, which are likely to exist in DRG neurons as in other cells [79][80][81].…”

Section: Discussionmentioning

confidence: 99%

Identification of miRNAs involved in DRG neurite outgrowth and their putative targets

et al. 2017

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Peripheral neurons regenerate their axons after injury. Transcriptional regulation by microRNAs (miRNAs) is one possible mechanism controlling regeneration. We profiled miRNA expression in mouse dorsal root ganglion (DRG) neurons after a sciatic nerve crush, and identified 49 differentially expressed miRNAs. We evaluated the functional role of each miRNA using a phenotypic analysis approach. To predict the targets of the miRNAs we employed RNA-Sequencing and examined transcription at the isoform level. We identify thousands of differentially expressed isoforms and bioinformatically associate the miRNAs that modulate neurite growth with their putative target isoforms to outline a network of regulatory events underlying peripheral nerve regeneration. MiR-298, let-7a and let-7f enhance neurite growth and target the majority of isoforms in the differentially expressed network.

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Section: Discussionmentioning

confidence: 99%

Identification of miRNAs involved in DRG neurite outgrowth and their putative targets

et al. 2017

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“…However, in CRC, miRNAs are also implicated in onset, development and metastasis [52][53][54]. Moreover, Chiang et al asserted that miRNA-192, miRNA-194 and miRNA-215 are correlated with augmented cancer size [55], while Yuan et al recognised 76 miRNAs as differentially expressed (DE) in CRC and normal tissues, including the oncogenic mirNA-17~92, miR-182, and miRNA-503 cluster.…”

Section: Microbiota and Colorectal Cancermentioning

confidence: 99%

Interactions between the MicroRNAs and Microbiota in Cancer Development: Roles and Therapeutic Opportunities

Allegra

Musolino

Tonacci

et al. 2020

Cancers

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The human microbiota is made up of the fungi, bacteria, protozoa and viruses cohabiting within the human body. An altered microbiota can provoke diseases such as cancer. The mechanisms by which a modified microbiota can intervene in the onset and progression of neoplastic diseases are manifold. For instance, these include the effects on the immune system and the onset of obesity. A different mechanism seems to be constituted by the continuous and bidirectional relationships existing between microbiota and miRNAs. MiRNAs emerged as a novel group of small endogenous non-coding RNAs from that control gene expression. Several works seem to confirm the presence of a close connection between microbiota and miRNAs. Although the main literature data concern the correlations between microbiota, miRNAs and colon cancer, several researches have revealed the presence of connections with other types of tumour, including the ovarian tumour, cervical carcinoma, hepatic carcinoma, neoplastic pathologies of the central nervous system and the possible implication of the microbiota-miRNAs system on the response to the treatment of neoplastic pathologies. In this review, we summarise the physiological and pathological functions of the microbiota on cancer onset by governing miRNA production. A better knowledge of the bidirectional relationships existing between microbiota and miRNAs could provide new markers for the diagnosis, staging and monitoring of cancer and seems to be a promising approach for antagomir-guided approaches as therapeutic agents.

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“…In humans, several miRNAs have been shown implicated in controlling lipid homeostasis and inflammation [9,10], which has led to several studies of miRNA profiling as noninvasive biomarkers for early detection of asymptomatic and symptomatic atherosclerotic lesions [11,12]. Importantly, miRNAs are responsive to environmental factors [13] and exhibit temporal expression patterns [14], supporting the possibility of acute changes in miRNA expression during fed states. Because the consumption of saturated fatty acids (SFAs) has been linked with larger and longer postprandial hypertriglyceridemic response [15] and increased risk for several chronic diseases such as cardiovascular disease and cancer [16,17], the aims of this study were to explore whether a high-saturated fat meal, mainly rich in palmitic acid, could change the miRNA signature in peripheral blood mononuclear cells (PBMCs) from healthy individuals in the postprandial period and to establish the role of miRNA signature as a novel tool to predict clinical outcomes linking the postprandial metabolism of dietary SFAs with pathophysiological processes.…”

Section: Introductionmentioning

confidence: 99%

A microRNA expression signature of the postprandial state in response to a high-saturated-fat challenge

López

Bermúdez

Paz

et al. 2018

The Journal of Nutritional Biochemistry

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The postprandial hypertriglyceridemia is an important and largely silent disturbance involved in the genesis of numerous pathological conditions. Exaggerated and prolonged states of postprandial hypertriglyceridemia are frequently related to the ingestion of meals enriched in saturated fatty acids (SFAs). MicroRNAs are noncoding RNAs that function as gene regulators and play significant roles in both health and disease. However, differential miRNA expression between fasting and postprandial states has never been elucidated. Here, we studied the impact of a high-saturated-fat meal, mainly rich in palmitic acid, on the miRNA signature in peripheral blood mononuclear cells (PBMCs) of nine male healthy individuals in the postprandial period by using a two-step analysis: miRNA array and validation through quantitative real-time polymerase chain reaction. Compared with miRNA expression signature in PBMCs at fasting, 36 miRNAs were down-regulated and 43 miRNAs were up-regulated in PBMCs at postprandial hypertriglyceridemic peak. Six chromosomes (3, 7, 8, 12, 14 and 19) had nearly half (48.1%) of dysregulated miRNA-gene-containing regions. Down-regulated miR-300 and miR-369-3p and up-regulated miR-495-3p, miR-129-5p and miR-7-2-3p had the highest number of target genes. The differentially expressed miRNAs and their predicted target genes involved pathways in cancer, MAPK signaling pathway, endocytosis and axon guidance. Only down-regulated miRNAs notably targeted PI3K-Akt signaling pathways, whereas only up-regulated miRNAs targeted focal adhesion, Wnt signaling pathway, transcriptional misregulation in cancer and ubiquitin-mediated proteolysis. This is the first study of miRNA expression analysis of human PBMCs during postprandial hypertriglyceridemia and offers insight into new potential mechanisms by which dietary SFAs influence health or disease.

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Manipulating MiRNA Expression: a Novel Approach for Colon Cancer Prevention and Chemotherapy

Cited by 24 publications

References 151 publications

Identification of miRNAs involved in DRG neurite outgrowth and their putative targets

Identification of miRNAs involved in DRG neurite outgrowth and their putative targets

Interactions between the MicroRNAs and Microbiota in Cancer Development: Roles and Therapeutic Opportunities

A microRNA expression signature of the postprandial state in response to a high-saturated-fat challenge

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