Manipulating resistance to mungbean yellow mosaic virus in greengram (Vigna radiata L): Through CRISPR/Cas9 mediated editing of the viral genome

Talakayala, Ashwini; Mekala, Gopala Krishna; Reddy, Malireddy K.; Ankanagari, Srinivas; Mallikarjuna, G. B.

doi:10.3389/fsufs.2022.911574

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…The approach of Kumar et al (2017) and Sahoo et al (2016) of overexpressing a gene originally found in Arabidopsis in mungbean highlights the importance of using knowledge from model species to accelerate mungbean improvement. Gene editing approaches are now available in mungbean and have been applied for biotic stress (Talakayala et al, 2022). One advantage of genome editing is that plants generated with gene knockout strategies (classified as SDN1) are now considered non‐GM in many jurisdictions (Zhang et al, 2021).…”

Section: Breeding For Adaptation To Abiotic Stress In Mungbeanmentioning

confidence: 99%

Building a better Mungbean: Breeding for reproductive resilience in a changing climate

Haeften

Dudley

Kang

et al. 2023

Food and Energy Security

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Mungbean (Vigna radiata (L.) R. Wilczek var. radiata) is a significant food and cash crop grown in tropical and subtropical regions. Mungbean production and consumer demand have increased substantially over the last two decades, owing to its agronomic, nutritional and economic benefits. Despite increased breeding efforts and the expansion of mungbean production in various agro‐climatic regions, further production is hindered by low yield and variability, which is partly attributed to the impacts of abiotic stress. Abiotic stress impacts on the physiology, morphology and reproductive ability of mungbean which influences yield. Exposure to abiotic stresses at the reproductive stage is considered the most critical for yield production. In this review, we evaluate how abiotic stress impacts mungbean growth and productivity when occurring during the reproductive stage and traits that may confer adaptation. We present the limitations of current research including limited number of genotypes, lack of field experiments and detailed experimental information. We highlight the opportunities to exploit new tools and technologies, such as high‐throughput phenotyping platforms, gene editing, and genomic selection, to accelerate breeding efforts to develop more resilient mungbean cultivars for today and tomorrow.

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Section: Breeding For Adaptation To Abiotic Stress In Mungbeanmentioning

confidence: 99%

Building a better Mungbean: Breeding for reproductive resilience in a changing climate

Haeften

Dudley

Kang

et al. 2023

Food and Energy Security

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“…Thus far, CRISPR applications have been shown to be effective against bacterial, viral, and fungi-borne crop diseases, including powdery mildew in wheat and tomatoes, bacterial blight in rice, and bean yellow dwarf virus [80][81][82]. High-quality genome assembly and pan-genome studies to facilitate genetic discovery in mungbean and its improvement Talakayala et al [87] worked on manipulating resistance to mungbean yellow mosaic virus (MYMV) in greengram (Vigna radiata L) through CRISPR/Cas9-mediated editing of the viral genome. In their experiment, these authors confirmed that manipulation of resistance to MYMV through the editing of the pathogen genome using the CRISPR/Cas9 tool can be a powerful approach to combat viruses and develop resistance in greengram.…”

Section: Genomic-assisted Approaches For Promoting Resistance In Mung...mentioning

confidence: 99%

Progress in the Use of Combined Omics for Mungbean Breeding Improvement and Its Potential in Promoting Resistance against Cercospora Leaf Spot

Gudeta,

Keneni,

Figlan

2024

IJPB

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Cercospora leaf spot (CLS) is the most destructive fungal disease, deteriorating the production and productivity of mungbean (Vigna radiata (L.) Wilczek). Mungbean is one of the most nutritionally and environmentally important legumes, with popularity currently increasing as a ‘future smart food crop’ due to its several health benefits. In recent years, there has been considerable research progress in improving disease resistance in legumes. However, only a limited number of studies have pinpointed potential genes and candidate genes associated with resistance traits to CLS in mungbeans. Identifying the potential resistant resources through combined omics approaches is an efficient strategy to screen the best Cercospora-resistant mungbean varieties for further molecular breeding and improvement. Potential omics approaches are important tools to predict disease management strategies, alleviate chemical overuse, and mitigate problems due to malnutrition. Sustainable breeding research efforts using potential combined omics, including automated phenotyping, to promote important resistant traits associated with CLS in mungbeans are still unexplored and a key issue that needs to be addressed. Omics-technology-based research findings on resistance genes, proteins, and metabolites against CLS in mungbean are recognised in this review. Due to a limitation of research findings specifically underscoring the use of omics tools for screening resistant mungbean against CLS, best related research outcomes on other crops are included in this review.

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“…Reduced viral infection symptoms and viral RNA accumulation in the genome-edited plants were the results of targeting CMV and TMV with Francisella novicida Cas9 (FnCas9) in combination with particular sgRNAs ( Zhang et al, 2018 ). CRISPR-Cas9 was used to target single-stranded DNA-A of the AV1 and AC1 genes in the Mung bean yellow mosaic virus to enhance resistance in tobacco against this virus ( Talakayala et al, 2024 ). RNA viruses that harm plant cells can be targeted using the CRISPR-Cas13 system in addition to the CRISPR-Cas9 system.…”

Section: Genome Editing For Improving Disease Resistance Against Vira...mentioning

confidence: 99%

Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production

Manzoor,

Nabi,

Rather

et al. 2024

Front. Genome Ed.

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Modern agriculture has encountered several challenges in achieving constant yield stability especially due to disease outbreaks and lack of long-term disease-resistant crop cultivars. In the past, disease outbreaks in economically important crops had a major impact on food security and the economy. On the other hand climate-driven emergence of new pathovars or changes in their host specificity further poses a serious threat to sustainable agriculture. At present, chemical-based control strategies are frequently used to control microbial pathogens and pests, but they have detrimental impact on the environment and also resulted in the development of resistant phyto-pathogens. As a replacement, cultivating engineered disease-resistant crops can help to minimize the negative impact of regular pesticides on agriculture and the environment. Although traditional breeding and genetic engineering have been instrumental in crop disease improvement but they have certain limitations such as labour intensity, time consumption, and low efficiency. In this regard, genome editing has emerged as one of the potential tools for improving disease resistance in crops by targeting multiple traits with more accuracy and efficiency. For instance, genome editing techniques, such as CRISPR/Cas9, CRISPR/Cas13, base editing, TALENs, ZFNs, and meganucleases, have proved successful in improving disease resistance in crops through targeted mutagenesis, gene knockouts, knockdowns, modifications, and activation of target genes. CRISPR/Cas9 is unique among these techniques because of its remarkable efficacy, low risk of off-target repercussions, and ease of use. Some primary targets for developing CRISPR-mediated disease-resistant crops are host-susceptibility genes (the S gene method), resistance genes (R genes) and pathogen genetic material that prevents their development, broad-spectrum disease resistance. The use of genome editing methods has the potential to notably ameliorate crop disease resistance and transform agricultural practices in the future. This review highlights the impact of phyto-pathogens on agricultural productivity. Next, we discussed the tools for improving disease resistance while focusing on genome editing. We provided an update on the accomplishments of genome editing, and its potential to improve crop disease resistance against bacterial, fungal and viral pathogens in different crop systems. Finally, we highlighted the future challenges of genome editing in different crop systems for enhancing disease resistance.

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Manipulating resistance to mungbean yellow mosaic virus in greengram (Vigna radiata L): Through CRISPR/Cas9 mediated editing of the viral genome

Cited by 6 publications

References 32 publications

Building a better Mungbean: Breeding for reproductive resilience in a changing climate

Building a better Mungbean: Breeding for reproductive resilience in a changing climate

Progress in the Use of Combined Omics for Mungbean Breeding Improvement and Its Potential in Promoting Resistance against Cercospora Leaf Spot

Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production

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