Manipulation of a glycolytic regulator alters growth and carbon partitioning in the marine diatom Thalassiosira pseudonana

Abbriano, Raffaela M.; Vardar, Nurcan; Yee, Daniel P.; Hildebrand, Mark

doi:10.1016/j.algal.2018.03.018

Cited by 12 publications

(4 citation statements)

References 59 publications

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“…In this study, the transcription levels of FBP, PFK 1 ⃝, and PFK 2 ⃝ in the WP-containing medium were significantly higher than those in other media, and the results also indicated that the cells could activate the metabolic flows of both assimilation and dissimilation as sugar metabolism activation. Green algae are known to increase the gene transcript levels of FBP and PFK when sugar metabolism is activated [59], and this fact could reinforce the possibility that the enhancement in transcription levels of FPB and PFK meant activation of sugar metabolism in this study. GPMI and ENO are also important enzyme genes in glycolysis as the unique structural enzyme genes controlling the metabolic flow [60], and the fact that the transcription levels of those enzyme genes were improved in the WPcontaining medium compared to other media could support the idea of activating glycolysis by adding WP.…”

Section: Discussionsupporting

confidence: 75%

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Nakanishi,

Yomogita,

Horimoto

2024

Microorganisms

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Utilities of whey powder (WP) and whey protein concentrate 34% powder (WPC34) prepared as dairy-processing residues were evaluated using a green alga Chlamydomonas reinhardtii. Analysis of C. reinhardtii growth showed that the strain used WP and WPC34 as nitrogen sources. Its specific growth rate and maximum cell density in WP-containing medium were higher than those in WPC34-containing medium; growth with WPC34 was improved by adding KCl or K2HPO4, which content was decreased as a result of WPC34’s preparation from WP. Although the lipid contents in media containing dairy-processing residues were 2.72 ± 0.31 wt% and 2.62 ± 0.20 wt% with no significant difference, the composition ratio of fatty acid C14 with WPC34 was higher than that with WP and the composition ratio of the sum of fatty acid-C16 and -C18 with WPC34 tended to be lower than that with WP. Additionally, analyses of gene transcription showed that the transcription level of acetyl-CoA carboxylase biotin carboxyl carrier protein in WPC34-containing medium was lower than that in WP-containing medium, possibly affecting the ratios of the chain lengths of fatty acids. The transcription of genes involved in glycolysis and the TCA cycle was outstandingly lower in algae grown in WPC34-containing medium when compared to those cultivated in the presence of WP, resulting in differences in energy production for cell proliferation.

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Section: Discussionsupporting

confidence: 75%

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Nakanishi,

Yomogita,

Horimoto

2024

Microorganisms

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“…However, decades of foundational research on algal biochemistry and physiology (not reviewed here), may be leveraged to expedite the use of algae in biotechnology (Hildebrand et al, 2013). Efforts to progress the understanding of diverse algal traits has recently been bolstered by the advent of genome sequencing projects and functional genetic tools, revealing novel aspects of algal metabolism relevant to industrial applications (Moellering and Benning, 2010;Allen et al, 2011;Fabris et al, 2012;Kirst et al, 2012;Radakovits et al, 2012;Fabris et al, 2014;Abbriano et al, 2018;Luo et al, 2018;Pollier et al, 2019;Smith et al, 2019). Moreover, it is expected that knowledge on algal traits will be increasingly generated by the implementation of advanced synthetic and molecular biology approaches combined with phenomics.…”

Section: Resultsmentioning

confidence: 99%

Emerging Technologies in Algal Biotechnology: Toward the Establishment of a Sustainable, Algae-Based Bioeconomy

et al. 2020

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Mankind has recognized the value of land plants as renewable sources of food, medicine, and materials for millennia. Throughout human history, agricultural methods were continuously modified and improved to meet the changing needs of civilization. Today, our rapidly growing population requires further innovation to address the practical limitations and serious environmental concerns associated with current industrial and agricultural practices. Microalgae are a diverse group of unicellular photosynthetic organisms that are emerging as next-generation resources with the potential to address urgent industrial and agricultural demands. The extensive biological diversity of algae can be leveraged to produce a wealth of valuable bioproducts, either naturally or via genetic manipulation. Microalgae additionally possess a set of intrinsic advantages, such as low production costs, no requirement for arable land, and the capacity to grow rapidly in both large-scale outdoor systems and scalable, fully contained photobioreactors. Here, we review technical advancements, novel fields of application, and products in the field of algal biotechnology to illustrate how algae could present high-tech, low-cost, and environmentally friendly solutions to many current and future needs of our society. We discuss how emerging technologies such as synthetic biology, highthroughput phenomics, and the application of internet of things (IoT) automation to algal manufacturing technology can advance the understanding of algal biology and, ultimately, drive the establishment of an algal-based bioeconomy.

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“…For instance, C. reinhardtii with C allocation to structural and storage carbohydrates [ 28 ] uses the target of rapamycin to control the C-to-N balance in the cell, regulating growth and biomass accumulation [ 32 ]. On the other hand, P. tricornutum [ 33 ] and Thalassiosira pseudonana [ 34 ] rely on Gln synthetase/Glu synthase and fructose 2,6-bisphosphatase as C partitioning regulators. Further molecular genetic studies should test whether enzymes related to Phe and Tyr metabolism, including aromatic amino acid aminotransferase, arogenate dehydrogenase, and histidinol-phosphate aminotransferase, act as photoassimilate partitioning regulators or not.…”

Section: Discussionmentioning

confidence: 99%

Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina

et al. 2020

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In microalgae, photosynthesis provides energy and sugar phosphates for the biosynthesis of storage and structural carbohydrates, lipids, and nitrogenous proteins. The oleaginous alga Nannochloropsis salina does not preferentially partition photoassimilates among cellulose, chrysolaminarin, and lipids in response to nitrogenous nutrient deprivation. In the present study, we investigated whether genetic impairment of the cellulose synthase gene (CesA) expression would lead to protein accumulation without the accumulation of storage C polymers in N. salina. Three cesA mutants were generated by the CRISPR/Cas9 approach. Cell wall thickness and cellulose content were reduced in the cesA1 mutant, but not in cesA2 or cesA4 cells. CesA1 mutation resulted in a reduction of chrysolaminarin and neutral lipid contents, by 66.3% and 37.1%, respectively, but increased the soluble protein content by 1.8-fold. Further, N. salina cells with a thinned cell wall were susceptible to mechanical stress, resulting in a 1.7-fold enhancement of lipid extractability. Taken together, the previous and current studies strongly suggest the presence of a controlling mechanism that regulates photoassimilate partitioning toward C and N metabolic pathways as well as the cellulose metabolism as a potential target for cost-effective microalgal cell disruption and as a useful protein production platform.

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Manipulation of a glycolytic regulator alters growth and carbon partitioning in the marine diatom Thalassiosira pseudonana

Cited by 12 publications

References 59 publications

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Evaluation of Cellular Responses by Chlamydomonas reinhardtii in Media Containing Dairy-Processing Residues Derived from Cheese as Nutrients by Analyzing Cell Growth Activity and Comprehensive Gene Transcription Levels

Emerging Technologies in Algal Biotechnology: Toward the Establishment of a Sustainable, Algae-Based Bioeconomy

Genetic Impairment of Cellulose Biosynthesis Increases Cell Wall Fragility and Improves Lipid Extractability from Oleaginous Alga Nannochloropsis salina

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