1992
DOI: 10.1111/j.1471-4159.1992.tb08890.x
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Manipulation of Intracellular Calcium in NCB‐20 Cells

Abstract: A number of lines of evidence indicate that the Ca2+ and cyclic AMP signalling systems interact in NCB-20 cells. However, to date, the regulation of [Ca2+]i homeostasis has not been studied in this cell line. The present study aimed to clarify our understanding of [Ca2+]i homeostasis in these cells and to evaluate tools that manipulate [Ca2+]i, independently of protein kinase C effects. Bradykinin, by a B2-receptor, elevated [Ca2+]i by a pertussis-toxin-insensitive mechanism. The BK-stimulated [Ca2+]i rise ori… Show more

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Cited by 14 publications
(11 citation statements)
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“…Accumulation of cAMP in intact cells was measured according to the method of Evans et al (Evans et al, 1984) as described previously (Garritsen and Cooper, 1992) with some modifications. At 2 days post-transfection, HEK293 cells were incubated in MEM (90 minutes at 37°C) with 2-3 H-adenine (1.5 Ci per well) to label the ATP pool.…”
Section: Cell Population Measurement Of Camp Accumulationmentioning
confidence: 99%
See 1 more Smart Citation
“…Accumulation of cAMP in intact cells was measured according to the method of Evans et al (Evans et al, 1984) as described previously (Garritsen and Cooper, 1992) with some modifications. At 2 days post-transfection, HEK293 cells were incubated in MEM (90 minutes at 37°C) with 2-3 H-adenine (1.5 Ci per well) to label the ATP pool.…”
Section: Cell Population Measurement Of Camp Accumulationmentioning
confidence: 99%
“…Assays were terminated by the addition of ice-cold 5% (w/v final concentration) trichloroacetic acid. 3 H-cAMP was formed from 3 H-ATP and quantified as previously described (Evans et al, 1984;Garritsen and Cooper, 1992). Results are presented as the means ± s.d.…”
Section: Cell Population Measurement Of Camp Accumulationmentioning
confidence: 99%
“…It has been established that receptor activation caused a rapid PI breakdown in several tissues in response to stimuli, such as neurotransmitters, growth factors, hormones, and light (Rana & Hokin, 1990). BK‐induced hydrolysis of PI, with the subsequent generation of IPs and DAG and the rise in [Ca 2+ ] i , can be attenuated by short‐term activation of PKC in several cell types (Osugi et al , 1987; Garristen & Cooper, 1992; Yang et al , 1995; Sena et al , 1996; Yang et al , 1997). Furthermore, such a modulation of signal transduction in Ca 2+ ‐mobilizing cells by PKC has been proposed to be involved in homologous desensitization in DDT1 MF‐2 cells (Leeb‐Lundberg et al , 1985), vascular smooth muscle cells (Pfeilschifter et al , 1989) and tracheal smooth muscle cells (Yang et al , 1995, 1997).…”
Section: Discussionmentioning
confidence: 99%
“…In many cell types, including the neuroblastoma‐glioma hybrid NG108‐15 (Osugi et al , 1987), glioma C6‐4‐2 (Reiser et al , 1990), human astrocytoma cell line D384 (Balmforth et al , 1992), and tracheal smooth muscle cells (March & Hill, 1992; Yang et al , 1994a), BK receptors activate phospholipase C (PLC)‐mediated phosphoinositide (PI) hydrolysis in the plasma membrane. The resultant increase in IP 3 releases Ca 2+ from internal stores in several types of cells including the NG108‐15 (MacNicol & Schulman, 1992), endothelial cell (Buchan & Martin, 1991), NCB‐20 cells (Garristen & Cooper, 1992), and tracheal smooth muscle cells (Marsh & Hill, 1993; Yang et al , 1994b). Recently, we have reported that BK induces an increase in PI hydrolysis in canine cultured tracheal epithelial cells (TECs) which appears to be mediated via activation of the B 2 receptors (Luo et al , 1996).…”
Section: Introductionmentioning
confidence: 99%
“…The resultant pH values were in good agreement with those evaluated from an in vitro calibration of BCECF free acid. Fura-2 340/380 nm fluorescence ratios were converted to [Ca 2ϩ ] i values as described previously (29). Reverse Transcription-PCR Experiments-RNA was isolated with the SV total RNA isolation system (Promega).…”
Section: Methodsmentioning
confidence: 99%