Manipulation of Neural Circuits in Drosophila Larvae

Tastekin, Ibrahim; Louis, Matthieu

doi:10.1007/978-3-319-57363-2_6

Cited by 2 publications

(2 citation statements)

References 52 publications

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“…A series of 8 consecutive light flashes produced eight transitions from a run (high-tail speed) to a stop-turn (low-tail speed) ( Figure 3—figure supplement 2A ). We adjusted the intensity of the red light to elicit stopping behavior in at least 75% of the flashes ( Figure 3—figure supplement 2B and D ) and focused on an intensity of 18 W/m 2 to minimize artefactual stops due to startle responses elicited by sudden exposure to intense red light ( Tastekin and Louis, 2017 ). Averaging the time course of the tail-speed over 280 flashes yielded a stereotyped decrease in tail speed associated with the interruption of forward locomotion ( Figure 3E ).…”

Section: Resultsmentioning

confidence: 99%

Sensorimotor pathway controlling stopping behavior during chemotaxis in the Drosophila melanogaster larva

Tastekin

Khandelwal

Tadres

et al. 2018

eLife

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Sensory navigation results from coordinated transitions between distinct behavioral programs. During chemotaxis in the Drosophila melanogaster larva, the detection of positive odor gradients extends runs while negative gradients promote stops and turns. This algorithm represents a foundation for the control of sensory navigation across phyla. In the present work, we identified an olfactory descending neuron, PDM-DN, which plays a pivotal role in the organization of stops and turns in response to the detection of graded changes in odor concentrations. Artificial activation of this descending neuron induces deterministic stops followed by the initiation of turning maneuvers through head casts. Using electron microscopy, we reconstructed the main pathway that connects the PDM-DN neuron to the peripheral olfactory system and to the pre-motor circuit responsible for the actuation of forward peristalsis. Our results set the stage for a detailed mechanistic analysis of the sensorimotor conversion of graded olfactory inputs into action selection to perform goal-oriented navigation.

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Section: Resultsmentioning

confidence: 99%

Sensorimotor pathway controlling stopping behavior during chemotaxis in the Drosophila melanogaster larva

Tastekin

Khandelwal

Tadres

et al. 2018

eLife

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“…Although stochasticity is not an uncommon feature of many expression systems (Bohm et al, 2010; Tastekin and Louis, 2017), the variability of expression generated by SpaRCLIn was notable. Even for intersections that reliably produce very similar expression across animals, it is not common to get exactly the same pattern twice.…”

Section: Discussionmentioning

confidence: 99%

Cre-assisted Fine-mapping of Neural Circuits using Orthogonal Split Inteins

Luan

Kuzin

Odenwald

et al. 2019

Preprint

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Summary:Genetic methods for targeting small numbers of neurons of a specific type are critical for mapping the brain circuits underlying behavior. Existing methods can provide exquisite targeting precision in favorable cases, but for many cases alternative techniques will be required. Here, we introduce a new step-wise combinatorial method for sequentially refining neuronal targeting: Depending on the restriction achieved at the first step, a second step can be easily implemented to further refine expression. For both steps, the new method relies on two independent intersections. The primary intersection targets neurons based on their developmental origins (i.e. lineage) and terminal identities, while the second intersection limits the number of lineages represented in the primary intersection by selecting lineages with overlapping activity of two distinct enhancers during neurogenesis. Our method relies critically on two libraries of 134 transgenic fly lines that express fragments of a split Cre recombinase under the control of distinct neuroblast enhancers. The split Cre fragments are fused to non-interacting pairs of split inteins, which ensure reconstitution of full-length and active Cre when all fragments are expressed in the same cell. Our split Cre system, together with its open source libraries, represent off-the-shelf components that should facilitate the targeting and characterization of brain circuits in Drosophila. Our methodology may also prove useful in other genetic model organisms.

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Manipulation of Neural Circuits in Drosophila Larvae

Cited by 2 publications

References 52 publications

Sensorimotor pathway controlling stopping behavior during chemotaxis in the Drosophila melanogaster larva

Sensorimotor pathway controlling stopping behavior during chemotaxis in the Drosophila melanogaster larva

Cre-assisted Fine-mapping of Neural Circuits using Orthogonal Split Inteins

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