Manipulation of sporophytic self-incompatibility in plant breeding

Hinata, Kokichi; Isogai, Akira; Isuzugawa, Kanji

doi:10.1007/978-94-017-1669-7_6

Cited by 10 publications

(7 citation statements)

References 54 publications

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“…Intercrossing such hybrids could enhance natural rubber and inulin production via hybrid vigor, which is a widespread crop breeding strategy [ 13 ]. SC plants enable the crossing of otherwise non-crossable cultivars (expressing the same S -haplotype) to generate progeny from selfing [ 14 ]. Furthermore, they are easier to maintain and evaluate, and are less dependent on insect pollination, as shown by comparing SC domestic and SI wild-type cultivars of sunflower ( Helianthus annuus ) [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz

Wollenweber¹,

Deenen²,

Roelfs

et al. 2021

Plants

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The transition of the Russian dandelion Taraxacum koksaghyz (Asteraceae) to a profitable, alternative crop producing natural rubber and inulin requires the optimization of several agronomic traits, cultivation conditions and harvesting procedures to improve the yield. However, efficient breeding is hindered by the obligatory sexual outcrossing of this species. Several other asters have been investigated to determine the mechanism of self-incompatibility, but the underlying molecular basis remains unclear. We therefore investigated the self-pollination and cross-pollination of two compatible T. koksaghyz varieties (TkMS2 and TkMS3) by microscopy and transcriptomic analysis to shed light on the pollination process. Self-pollination showed typical sporophytic self-incompatibility characteristics, with the rare pollen swelling at the pollen tube apex. In contrast, cross-pollination was characterized by pollen germination and penetration of the stigma by the growing pollen tubes. RNA-Seq was used to profile gene expression in the floret tissue during self-pollination and cross-pollination, and the differentially expressed genes were identified. This revealed three candidates for the early regulation of pollination in T. koksaghyz, which can be used to examine self-incompatibility mechanisms in more detail and to facilitate breeding programs.

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Section: Introductionmentioning

confidence: 99%

Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz

Wollenweber¹,

Deenen²,

Roelfs

et al. 2021

Plants

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“…The genomic relationship among Brassica species is known as "U's triangle" (U, 1935), in which B. napus (AACC) is an amphidiploid species developed from Aand C-genome donor diploid species, B. rapa (AA) and B. oleracea (CC). Brassica rapa and B. oleracea show self-incompatibility (SI), whereas B. napus shows selfcompatibility (SC) (Hinata et al, 1994). B. napus is known as oilseed rape, and is one of the most important high-yield crops in the world (FAO statistics; http://www.…”

Section: Introductionmentioning

confidence: 99%

Characterization of self-incompatible <i>Brassica napus</i> lines lacking <i>SP11</i> expression

Okamoto

Hikichi

et al. 2020

Genes Genet. Syst.

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Recognition of self-incompatibility (SI) is regulated by the SRK and SP11 genes in Brassicaceae. Brassica rapa and B. oleracea are self-incompatible, while most cultivated species of B. napus, which arose from hybridization between B. rapa and B. oleracea, are self-compatible. Various studies of the SRK and SP11 genes in self-compatible B. napus have been reported, but details of the mechanism in different B. napus lines are not fully understood. In this study, we confirmed the S haplotypes, SI phenotypes and SP11 expression in 10 representative lines of B. napus, and identified two SI lines (N110 and N343) lacking SP11 expression. In N343 (with BnS 1 and BnS 6 haplotypes), we confirmed that there is a 3.6-kb insertion in the promoter region of BnSP11-1, and that BnSP11-1 and BnSP11-6 are not expressed, as reported previously (expression of BnSP11-6 is suppressed by the BnS 1 haplotype), although this line is self-incompatible. Similarly, in N110, with two novel S haplotypes (BnS 8 and BnS 9 ) in addition to BnS 6 , a 4.3-kb insertion was identified in the promoter region of BnSP11-9, and expression levels of BnSP11-6, BnSP11-8 and BnSP11-9 were all suppressed (BnSP11-6 and BnSP11-8 may be suppressed by BnS 8 and BnS 9 , respectively), although the phenotype was selfincompatible. This observation of an SI phenotype without SP11 expression suggests the existence of unknown factor(s) that induce pollen-stigma incompatibility in B. napus.

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“…It is now clear that SI evolved numerous times during angiosperm evolution (de Nettancourt, ). Resolving the site and timing of self‐rejection is important for understanding the origin of SI in plants (Sage et al ., ), understanding the functional consequences of SI (Vaughton & Ramsey, ) and for overcoming SI in breeding programmes (Hinata, Isogai & Isuzugawa, ). Particularly controversial is late‐acting self‐incompatibility (LSI), in which rejection is proposed to occur in the ovary rather than the stigma or style (Sears, ; Knight & Rogers, ; Cope, ; Dulberger, ; Seavey & Bawa, ; Sage, Bertin & Williams, ; Sage et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Breeding systems inClivia(Amaryllidaceae): late-acting self-incompatibility and its functional consequences

Kiepiel

Johnson

2014

Bot J Linn Soc

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Late-acting (ovarian) self-incompatibility, characterized by minimal or zero seed production following self-pollen tube growth to the ovules, is expected to show phylogenetic clustering, but can otherwise be difficult to distinguish from early-acting inbreeding depression. In Amaryllidaceae, late-acting self-incompatibility has been proposed for Narcissus (Narcisseae) and Cyrtanthus (Cyrtantheae). Here, we investigate whether it occurs in the horticulturally important genus Clivia (Haemantheae) and test whether species in this genus experience ovule discounting in wild populations. Seed-set results following controlled hand pollinations revealed that Clivia miniata and C. gardenii are largely self-sterile. Self-and cross-pollinated flowers of both species had similar proportions of pollen tubes entering the ovary, and those of C. gardenii also did not differ in the proportions of pollen tubes that penetrated ovules, thus ruling out classical gametophytic self-incompatibility acting in the style, but not early inbreeding depression. Flowers that received equal mixtures of self-and cross-pollen set fewer seeds than those that received cross-pollen only, but it was unclear whether this effect was a result of ovule discounting or interactions on the stigma. The prevention of self-pollination by the emasculation of either single flowers or whole inflorescences in wild populations did not affect seed set, suggesting that ovule discounting is not a major natural limitation on seed production. Flowers typically produce one to three large fleshy seeds from approximately 16 available ovules, even when supplementally hand pollinated, suggesting that fecundity is mostly resource limited. The results of this study suggest that Clivia spp. are largely self-sterile as a result of either a late-acting self-incompatibility system or severe early inbreeding depression, but ovule discounting caused by self-pollination is not a major constraint on fecundity.

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Manipulation of sporophytic self-incompatibility in plant breeding

Cited by 10 publications

References 54 publications

Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz

Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz

Characterization of self-incompatible <i>Brassica napus</i> lines lacking <i>SP11</i> expression

Breeding systems inClivia(Amaryllidaceae): late-acting self-incompatibility and its functional consequences

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