2002
DOI: 10.1104/pp.010671
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Manipulation of Strawberry Fruit Softening by Antisense Expression of a Pectate Lyase Gene

Abstract: Strawberry (Fragaria ϫ ananassa, Duch., cv Chandler) is a soft fruit with a short postharvest life, mainly due to a rapid lost of firm texture. To control the strawberry fruit softening, we obtained transgenic plants that incorporate an antisense sequence of a strawberry pectate lyase gene under the control of the 35S promoter. Forty-one independent transgenic lines (Apel lines) were obtained, propagated in the greenhouse for agronomical analysis, and compared with control plants, non-transformed plants, and t… Show more

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Cited by 308 publications
(193 citation statements)
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“…Addition of auxin in rooting media accentuates rooting but also microcuttings developed callus at their base that hamper their field establishment. Similar results on the rooting and subsequent field establishment were also reported by several researchers (4,24,28,29). While, it has been recognized that the propagation through tissue culture must proceed through a sequence of steps such as, (1) …”
Section: Resultssupporting
confidence: 74%
“…Addition of auxin in rooting media accentuates rooting but also microcuttings developed callus at their base that hamper their field establishment. Similar results on the rooting and subsequent field establishment were also reported by several researchers (4,24,28,29). While, it has been recognized that the propagation through tissue culture must proceed through a sequence of steps such as, (1) …”
Section: Resultssupporting
confidence: 74%
“…The role of PL in fruit firmness was further demonstrated by transgenic strawberries with transformation of antisense PL (Jiménez-Bermúdez et al 2002;Sesmero et al 2007). The transgenic fruits containd less pectin and were firmer while the softening process during fruit ripening was significantly delayed.…”
Section: Discussionmentioning
confidence: 99%
“…Histological observation revealed smaller intercellular spaces and larger contact areas between adjacent cells in transgenic fruits (Santiago-Doménech et al 2008;Youssef et al 2009). These alterations had a positive effect on fruit firmness and in the maintenance of cell structure, therefore, PL can be considered as a key candidate gene to increase firmness and prevent fruit softening (Jiménez-Bermúdez et al 2002;Qian et al 2009). FaSCPL and FaTPL isolated from Sweet Charlie and Toyonoka respectively, may contribute to the functional identification of PL through the genetic transformation system.…”
Section: Discussionmentioning
confidence: 99%
“…Experiments with pectolytic enzymes have deemphasized the role of pectins in fruit softening (Jiménez-Bermúdez et al, 2002). Pectin degrading enzymes such as PG, PME, pectate lyase (PL), and rhamnogalacturonase (RG) are the most implicated in fruit-tissue softening (Prasanna et al, 2007).…”
Section: Role Of Pectin Degrading Enzymesmentioning
confidence: 99%
“…One group includes those fruits that soften greatly during ripening, acquiring a melting texture, whereas the other group comprises fruits that soften moderately and display a crisp Review Article fracturable texture. Strawberry (Fragaria × ananassa, Duch., cv Chandler) is included in the first group, joint to other economically important crops such as tomato (Lycopersicon esculentum) and avocado (Persea americana) (Jiménez-Bermúdez et al, 2002).…”
Section: Introductionmentioning
confidence: 99%