Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates

Cui, Long-Hui; Joo, Hyung Joon; Kim, Dae Hwan; Seo, Ha-Rim; Kim, Jung Suk; Choi, Seung-Cheol; Huang, Lihua; Na, Ji Eun; Lim, I-Rang; Kim, Jong-Ho; Rhyu, Im Joo; Hong, Soon Jun; Lee, Kyu Back; Lim, Do–Sun

doi:10.1016/j.actbio.2017.10.026

Cited by 15 publications

(11 citation statements)

References 44 publications

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“…Likewise, a nano-architecture substrate will allow cells to sense structural cues, which triggers a similar cellular response observed when cells are in their natural environment surrounded by the ECM ( Selvakumaran et al, 2008 ; Yoo et al, 2015 ; Schulte et al, 2016a ; Yang et al, 2017 ). The nano-architecture surfaces provide cues to initiate the production of ECM molecules necessary for initial cell attachment to surfaces ( Cui et al, 2018 ). Furthermore, the nano-architecture surface results in an increased adsorption of those ECM molecules necessary for cell attachment ( Salakhutdinov et al, 2008 ; Ereifej et al, 2013b ; Woeppel et al, 2018 ).…”

Section: The Role Of Architecture For Brain Homeostasis and Physiologmentioning

confidence: 99%

Nano-Architectural Approaches for Improved Intracortical Interface Technologies

et al. 2018

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Intracortical microelectrodes (IME) are neural devices that initially were designed to function as neuroscience tools to enable researchers to understand the nervous system. Over the years, technology that aids interfacing with the nervous system has allowed the ability to treat patients with a wide range of neurological injuries and diseases. Despite the substantial success that has been demonstrated using IME in neural interface applications, these implants eventually fail due to loss of quality recording signals. Recent strategies to improve interfacing with the nervous system have been inspired by methods that mimic the native tissue. This review focusses on one strategy in particular, nano-architecture, a term we introduce that encompasses the approach of roughening the surface of the implant. Various nano-architecture approaches have been hypothesized to improve the biocompatibility of IMEs, enhance the recording quality, and increase the longevity of the implant. This review will begin by introducing IME technology and discuss the challenges facing the clinical deployment of IME technology. The biological inspiration of nano-architecture approaches will be explained as well as leading fabrication methods used to create nano-architecture and their limitations. A review of the effects of nano-architecture surfaces on neural cells will be examined, depicting the various cellular responses to these modified surfaces in both in vitro and pre-clinical models. The proposed mechanism elucidating the ability of nano-architectures to influence cellular phenotype will be considered. Finally, the frontiers of next generation nano-architecture IMEs will be identified, with perspective given on the future impact of this interfacing approach.

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Section: The Role Of Architecture For Brain Homeostasis and Physiologmentioning

confidence: 99%

Nano-Architectural Approaches for Improved Intracortical Interface Technologies

et al. 2018

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“…In the last decade, extensive efforts have been made to study the interactions of ECs, SMCs, or both with varying topography stimuli, with some reported to achieve selective modulation of the vascular cells. Among them, the influence of various surface morphology profiles including regular grooves [ 12 ], pillars [ 13 ], and pits [ 14 ], and irregular morphology of defined roughness parameters have been investigated [ 15 ], where substrates of polymers (PDMS, PVC, PS and PLA), ceramics (silicon, bulk metallic glass) and metals (Al 2 O 3 , TiO 2 , stainless steel and CoCr) were employed. For example, Ding et al reported 1 μm groove pattern on silicon wafers coated with a thin layer of titanium oxide was able to improve the ECs attachment and proliferation in vitro [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…The deployment of gradients allows for exploring the effect of continuously varying surface parameters within a single experiment [ 17 ], with minimized system errors and saved cost. Previous reports have demonstrated the feasibility and advantages of the surface gradient approach in the study of the response of a variety of cells, including endothelial colony-forming cells [ 13 ], MC3T3-E1 osteoblastic cells [ 18 ], and human mesenchymal stem cells [ 19 ]. While methods to fabricate morphological gradients mainly include particle binding, electrochemical etching, erosion/chemical polishing—replica [ 17 ], the substrates used are often limited to silicon or glass with entirely different characteristics from biodegradable materials.…”

Section: Introductionmentioning

confidence: 99%

Nano-micrometer surface roughness gradients reveal topographical influences on differentiating responses of vascular cells on biodegradable magnesium

Zhou

Zhang

et al. 2021

Bioactive Materials

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Distinctively directing endothelial cells (ECs) and smooth muscle cells (SMCs), potentially by surface topography cue, is of central importance for enhancing bioefficacy of vascular implants. For the first time, surface gradients with a broad range of nano-micrometer roughness are developed on Mg, a promising next-generation biodegradable metal, to carry out a systematic study on the response of ECs and SMCs. Cell adhesion, spreading, and proliferation are quantified along gradients by high-throughput imaging, illustrating drastic divergence between ECs and SMCs, especially in highly rough regions. The profound role of surface topography overcoming the biochemical cue of released Mg 2+ is unraveled at different roughness ranges for ECs and SMCs. Further insights into the underlying regulatory mechanism are gained at subcellular and gene levels. Our work enables high-efficient exploration of optimized surface morphology for modulating favored cell selectivity of promoting ECs and suppressing SMCs, providing a potential strategy to achieve rapid endothelialization for Mg.

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“…Low biotoxicity, nuclease resistance, relative stability and programmability [137] the cytoskeletal integrity and focal adhesion of hECFCs on flat plates, and filamentous outgrowth increased significantly in the range from 120 to 200 nm [93]. The pore size of nanomaterials also influences the morphology and adhesion of human endothelial cells.…”

Section: Dimensionsmentioning

confidence: 99%

“…Nanomaterials not only imitate the ECM but also change the surface pattern, hardness and elasticity of the scaffolds. Cui et al reported F-actin assembly and increased filopodia in hECFCs on gradient nanopattern plates through the activation of Rho-associated protein kinase signalling; hECFCs on flat plates did not express these proteins [93]. The nanostructure and substrate rigidity determine the adhesion, spreading, differentiation and tube formation of cardiac progenitor cells by changing YAP/TAZ expression [121].…”

Section: Regulating Cytoskeleton Rearrangementmentioning

confidence: 99%

Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications

Liu

Zhang

et al. 2020

J Nanobiotechnol

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The vascular system, which transports oxygen and nutrients, plays an important role in wound healing, cardiovascular disease treatment and bone tissue engineering. Angiogenesis is a complex and delicate regulatory process. Vascular cells, the extracellular matrix (ECM) and angiogenic factors are indispensable in the promotion of lumen formation and vascular maturation to support blood flow. However, the addition of growth factors or proteins involved in proangiogenic effects is not effective for regulating angiogenesis in different microenvironments. The construction of biomaterial scaffolds to achieve optimal growth conditions and earlier vascularization is undoubtedly one of the most important considerations and major challenges among engineering strategies. Nanomaterials have attracted much attention in biomedical applications due to their structure and unique photoelectric and catalytic properties. Nanomaterials not only serve as carriers that effectively deliver factors such as angiogenesis-related proteins and mRNA but also simulate the nano-topological structure of the primary ECM of blood vessels and stimulate the gene expression of angiogenic effects facilitating angiogenesis. Therefore, the introduction of nanomaterials to promote angiogenesis is a great helpful to the success of tissue regeneration and some ischaemic diseases. This review focuses on the angiogenic effects of nanoscaffolds in different types of tissue regeneration and discusses the influencing factors as well as possible related mechanisms of nanomaterials in endothelial neovascularization. It contributes novel insights into the design and development of novel nanomaterials for vascularization and therapeutic applications.

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Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates

Cited by 15 publications

References 44 publications

Nano-Architectural Approaches for Improved Intracortical Interface Technologies

Nano-Architectural Approaches for Improved Intracortical Interface Technologies

Nano-micrometer surface roughness gradients reveal topographical influences on differentiating responses of vascular cells on biodegradable magnesium

Insights into the angiogenic effects of nanomaterials: mechanisms involved and potential applications

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