Mannanoligosaccharide agglutination by Salmonella enterica strains isolated from carrier pigs

Borowsky, Luciane; Corção, Gertrudes; Cardoso, Marisa Ribeiro de Itapema

doi:10.1590/s1517-83822009000300007

Cited by 12 publications

(7 citation statements)

References 25 publications

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“…However, the lower expression of cytokines in piglets fed an HY diet could be related to the presence of several active compounds, for example, glutamine plays a role in cell proliferation, whereas β-glucans and their binding to specific cell receptors (e.g., Dectin-1 and roll-like receptors) activate antigen-presenting cells for pathogen prevention [18]. The MOS can also interfere with type 1 fimbria adhesion of enteric bacteria through binding itself to the bacteria [27]. This mechanism causes a reduction in toll-like receptor 4 in the Peyer's patches of the intestinal mucosa and thus lowers the secretion of pro-inflammatory cytokines [28].…”

Section: Immunoglobulin and Pro-inflammatory Cytokinesmentioning

confidence: 99%

Hydrolyzed Yeast Supplementation to Newly Weaned Piglets: Growth Performance, Gut Health, and Microbial Fermentation

Boontiam

Bunchasak

Cho

et al. 2022

Animals

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Hydrolyzed yeast (HY)-derived protein from Saccharomyces cerevisiae has a high digestible protein content and nucleotides and is a sweetener immunostimulatory substance. This could be used in nursery diets to minimize diarrhea and improve the growth rate and gut health of early-weaned piglets. This research was conducted with the objective of examining the effect of the inclusion level of HY as a potential protein ingredient for early-weaned piglets. A total of 72 crossbred weaned piglets [(Landrace × Large White) × Duroc] were assigned to three dietary treatments in six replicates with four pigs per pen. Dietary treatments were: (i) control (CON), piglets weaned at 18 days; (ii) CON diet with 5% HY inclusion (HY5); and (iii) CON diet with 10% HY inclusion (HY10) in a corn–soybean meal-based basal diet. Increasing HY levels positively improved body weight, average daily gain, and average daily feed intake (linear effect, p < 0.05). Furthermore, there was a linear increase in N-retention, albumin, jejunal villus height, villus height-to-crypt depth ratio, immunoglobulin A, acetate and propionate production, and Lactobacillus spp. count proportional to the dose of the HY-supplemented diet (p < 0.05). It also observed a decrease in diarrheal rate, jejunal crypt depth, blood urea nitrogen, pro-inflammatory cytokines, branched amino acids, and E. coli corresponding to the HY-supplemented levels (p < 0.05). However, the changes in the apparent total tract digestibility (dry matter, crude ash, and crude fat), blood glucose, butyrate, and Salmonella spp. were unaffected by the dietary HY level. Therefore, the supplementation of HY in the diet for early-weaned pigs not only supported the growth rate and immune function but also activated the beneficial bacterial growth of the early-weaned piglets.

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Section: Immunoglobulin and Pro-inflammatory Cytokinesmentioning

confidence: 99%

Hydrolyzed Yeast Supplementation to Newly Weaned Piglets: Growth Performance, Gut Health, and Microbial Fermentation

Boontiam

Bunchasak

Cho

et al. 2022

Animals

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“…There is compelling evidence for binding of MOS to FimH, a mannose specific lectin expressed in Gram negative bacteria with Type1 fimbriae, such as Salmonella 36 or pathogenic E. coli 37 38 . This competitive interaction significantly reduce bacterial adherence to intestinal epithelial cells bearing mannose-containing glycoprotein receptors.…”

Section: Discussionmentioning

confidence: 99%

Oligomannan Prebiotic Attenuates Immunological, Clinical and Behavioral Symptoms in Mouse Model of Inflammatory Bowel Disease

Ferenczi

Szegi

Winkler

et al. 2016

Sci Rep

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Inflammatory bowel disease shows increasing prevalence, however its pathomechanism and treatment is not fully resolved. Prebiotics are non-digestible carbohydrates which might provide an alternative to treat inflammatory conditions in the gut due to their positive effects either on the microbiome or through their direct effect on macrophages and mucosa. To test the protective effects of an oligomannan prebiotic, yeast cell wall mannooligosaccharide (MOS) was administered in dextran-sulphate-sodium (DSS)-induced mouse model of acute colitis. MOS reduced DSS-induced clinical- (weight loss, diarrhea) and histological scores (mucosal damage) as well as sickness-related anxiety. DSS treatment resulted in changes in colon microbiome with selective increase of Coliform bacteria. MOS administration attenuated colitis-related increase of Coliforms, normalized colonic muc2 expression and attenuated local expression of proinflammatory cytokines IL-1a, IL1b, IL6, KC, G-CSF and MCP1 as well as toll-like receptor TLR4 and NLRP3 inflammasome. Some of the protective effects of MOS were likely be mediated directly through local macrophages because MOS dose-dependently inhibited IL-1b and G-CSF induction following in vitro DSS challenge and IL1a, IL1b, G-SCF-, and IL6 increases after LPS treatment in mouse macrophage cell line RAW264.7. These results highlight oligomannan prebiotics as therapeutic functional food for testing in clinical trials.

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“…In our study, hemaggultination assay was performed on guinea pig erythrocytes with 1% mannose for type 1 fimbriae detection as described in previous study with the slight modification ( 18 ). A consent from institutional animal ethical committee of Interdisciplinary Biotechnology Unit (IBU), AMU (held on June 5, 2017, at IBU, AMU), was taken to perform this experiment.…”

Section: Methodsmentioning

confidence: 99%

CRISPRi Induced Suppression of Fimbriae Gene (fimH) of a Uropathogenic Escherichia coli: An Approach to Inhibit Microbial Biofilms

2017

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Urinary tract infection (UTI) is one the common infections caused by the recalcitrant nature of biofilms, developed after the pathogen has adhered to the inner lining of the urinary tract. Although significant research has been made in recent years to control these types of infection, but as of yet, no approach has sufficiently been able to reduce the prevalence of UTIs. The main objective of this study was to prevent UTIs through targeting the fimH gene, which is the major virulent factor responsible for biofilm formation. The novelty of this work lies in the use of CRISPRi, a gene specific editing tool to control such types of infections. Accordingly, the system was designed to target fimH gene, responsible for bacterial adherence and this approach was successfully validated by performing microscopic, biofilm and adherence assays.

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Mannanoligosaccharide agglutination by Salmonella enterica strains isolated from carrier pigs

Cited by 12 publications

References 25 publications

Hydrolyzed Yeast Supplementation to Newly Weaned Piglets: Growth Performance, Gut Health, and Microbial Fermentation

Hydrolyzed Yeast Supplementation to Newly Weaned Piglets: Growth Performance, Gut Health, and Microbial Fermentation

Oligomannan Prebiotic Attenuates Immunological, Clinical and Behavioral Symptoms in Mouse Model of Inflammatory Bowel Disease

CRISPRi Induced Suppression of Fimbriae Gene (fimH) of a Uropathogenic Escherichia coli: An Approach to Inhibit Microbial Biofilms

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