Mannitol catabolism by Staphylococcus aureus

Murphey, William H.; Rosenblum, E. D.

doi:10.1016/0003-9861(64)90332-7

Cited by 23 publications

(18 citation statements)

References 13 publications

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“…Using the devised system and specialized growth medium, the active metabolism of S. aureus could be monitored as the presence of phenol red in the growth medium allows for the development of colour change. The acetate that is produced during mannitol metabolism changes the medium colour gradually from red to yellow as the pH of the medium is lowered (Murphey and Rosenblum 1964).…”

Section: Discussionmentioning

confidence: 99%

System for real-time monitoring of mutation-in-progress

2008

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Aims: There has been an increasing number of pathogens becoming resistant to multiple classes of antibiotics. The study on how mutation emerges is therefore crucial to promote further understanding in this area. Conventional methods for such studies involve the monitoring of growth by standard plate count and biomolecular sequencing. This is however tedious and not cost effective. The aim of this paper is thus to introduce a novel system that enables real‐time monitoring of bacterial ‘mutation‐in‐progress’. Methods and Results: This system provides real‐time data, thus enabling confirmatory and further work to be performed at the important points when mutation is initiated. The system integrates spectroscopic techniques as the detection system and various supporting systems, such as a nutrient replenishing system, a pH control system and a waste system to allow for extended monitoring. In this paper, the feasibility of monitoring the emergence of ciprofloxacin resistance in Staphylococcus aureus was demonstrated as an initial example. The integrated system was found to require significantly less material resource and manpower compared with conventional techniques. Conclusions: The novel system to monitor bacterial mutation‐in‐progress is presented. The work reported herein demonstrates such a system to be effective and efficient in performing real‐time monitoring of mutation‐in‐progress, especially in extended time frames for mutation into the weeks and months. Significance and Impact of the Study: With the successful optimization of this system, researchers can learn about the dynamics of antibiotic resistance and further understand how the mutation of bacteria occurs.

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Section: Discussionmentioning

confidence: 99%

System for real-time monitoring of mutation-in-progress

2008

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“…Notably, it affects mannitol metabolism more strongly than it affects mannose metabolism. The principal difference between mannose and mannitol is the reduction of NAD ϩ as mannitol is metabolized to fructose-6-phoshate (16). Because a loss of electron transport limits the reoxidation of NADH, this means that the quantity of NAD ϩ is insufficient to allow the large number of dehydrogenase reactions to function efficiently (18).…”

Section: Discussionmentioning

confidence: 99%

Phenotype Microarray Profiling of Staphylococcus aureus menD and hemB Mutants with the Small-Colony-Variant Phenotype

et al. 2006

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Standard biochemical tests have revealed that hemin and menadione auxotrophic Staphylococcus aureus smallcolony variants (SCVs) exhibit multiple phenotypic changes. To provide a more complete analysis of the SCV phenotype, two genetically defined mutants with a stable SCV phenotype were comprehensively tested. These mutants, generated via mutations in menD or hemB that yielded menadione and hemin auxotrophs, were subjected to phenotype microarray (PM) analysis of over 1,500 phenotypes (including utilization of different carbon, nitrogen, phosphate, and sulfur sources; growth stimulation or inhibition by amino acids and other nutrients, osmolytes, and metabolic inhibitors; and susceptibility to antibiotics). Compared to parent strain COL, the hemB mutant was defective in utilization of a variety of carbon sources, including Krebs cycle intermediates and compounds that ultimately generate ATP via electron transport. The phenotype of the menD mutant was similar to that of the hemB mutant, but the defects in carbon metabolism were more pronounced than those seen with the hemB mutant. In both mutant strains, hexose phosphates and other carbohydrates that provide ATP in the absence of electron transport stimulated growth. Other phenotypes of SCV mutants, such as hypersensitivity to sodium selenite, sodium tellurite, and sodium nitrite, were also uncovered by the PM analysis. Key results of the PM analysis were confirmed in independent growth studies and by using Etest strips for susceptibility testing. PM technology is a new and efficient technology for assessing cellular phenotypes in S. aureus.

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“…On the basis of the ability to form an NADlinked mannitol-l-phosphate dehydrogenase, the following organisms have been postulated to employ scheme I: A. aerogenes (13,24), Bacillus subtilis (6), Diplococcus pneumoniae (15), E. coli (24, 25), Lactobacillus plantarum (2, 3, 24), Salmonella gallinarum (26), and Staphylococcus aureus (17). The failure to demonstrate mannitol phosphorylating activity in the extracts of most of these organisms might be due to the requirement for the unusual phosphoryl donor system.…”

Section: Discussionmentioning

confidence: 99%

Replacement of a Phosphoenolpyruvate-dependent Phosphotransferase by a Nicotinamide Adenine Dinucleotide-linked Dehydrogenase for the Utilization of Mannitol

1967

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Mannitol is dissimilated by Aerobacter aerogenes via an inducible pathway initiated by a phosphotransferase system dependent upon phosphoenolpyruvate as the phosphoryl donor. A mutational block in this pathway can be suppressed either at the phenotypic level by induction of D-arabitol dehydrogenase, an enzyme fortuitously capable of converting mannitol to fructose, or genotypically by a constitutive mutation in the D-arabitol system. Chemicals. Mannitol-l-phosphate, prepared according to the method described by Seegmiller and Horecker (22), was a gift of R.

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Mannitol catabolism by Staphylococcus aureus

Cited by 23 publications

References 13 publications

System for real-time monitoring of mutation-in-progress

System for real-time monitoring of mutation-in-progress

Phenotype Microarray Profiling of Staphylococcus aureus menD and hemB Mutants with the Small-Colony-Variant Phenotype

Replacement of a Phosphoenolpyruvate-dependent Phosphotransferase by a Nicotinamide Adenine Dinucleotide-linked Dehydrogenase for the Utilization of Mannitol

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