1985
DOI: 10.1099/00221287-131-11-2885
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Mannitol Metabolism in Agaricus bisporus: Purification and Properties of Mannitol Dehydrogenase

Abstract: Mannitol dehydrogenase (EC 1 . 1 . 1 .138) has been purified to homogeneity from fruit bodies of Agaricus bisporus. M , values of 115000 were determined by gel filtration and 130000 by rate zonal ultracentrifugation. The sedimentation coefficient is 6.5s. The native protein is composed of four subunits of M , 29000. The enzyme is specific for NADP, and shows low activity with sorbitol. Normal Michaelis-Menten kinetics are exhibited for both mannitol and NADP, giving K , values of 16-2 mM and 36 p~ respectively… Show more

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Cited by 16 publications
(18 citation statements)
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References 7 publications
(5 reference statements)
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“…However, the absence of any MAD1 transcript in nongerminated and germinated spores (Hahn and Mendgen, 1997) indicates that MAD1p present in spores will consequently be diluted and possibly degraded during germination. The thermodynamic calculations reported in this article are in harmony with the numbers calculated for the A. bisporus enzyme (Morton et al, 1985) and indicate that the equilibrium favors the formation of mannitol. In haustoria, the level of Fru may be considered fairly high, since Fru is taken up by haustoria through the action of HXT1p (Voegele et al, 2001).…”
Section: Discussionsupporting
confidence: 79%
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“…However, the absence of any MAD1 transcript in nongerminated and germinated spores (Hahn and Mendgen, 1997) indicates that MAD1p present in spores will consequently be diluted and possibly degraded during germination. The thermodynamic calculations reported in this article are in harmony with the numbers calculated for the A. bisporus enzyme (Morton et al, 1985) and indicate that the equilibrium favors the formation of mannitol. In haustoria, the level of Fru may be considered fairly high, since Fru is taken up by haustoria through the action of HXT1p (Voegele et al, 2001).…”
Section: Discussionsupporting
confidence: 79%
“…The enzyme showed typical characteristics for a mannitol:NADP 1 2-oxidoreductase (EC 1.1.1.138), both when expressed in yeast as well as in the native system. Kinetic parameters were very similar to the A. bisporus enzyme (Morton et al, 1985). With regard to other rust fungi, a NAD 1 -dependent MTD activity was observed in axenic cultures of P. graminis f. sp.…”
Section: Discussionsupporting
confidence: 61%
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“…The M6PR functions primarily in a synthetic manner, with only very low rates of mannitol-1-P oxidation observed . M6PR is distinctly different from mannitol-metabolizing enzymes in lower organisms, a11 of which can be characterized as 2-oxidoreductases, catalyzing the interconversion of Fru or Fru-6-P to mannitol or mannitol-1-P (Martinez et al, 1963;Ueng et al, 1976;Niehaus and Dilts, 1982;Morton et al, 1985;Teschner et al, 1990). Many of the mannitol dehydrogenases in lower organisms can function in both directions, e.g.…”
mentioning
confidence: 99%
“…In this regard, wide variation is encountered in the substrate specificities of polyol dehydrogenases studied in other organisms, with some enzymes oxidizing a broad spectrum of substrates, including non-physiological polyols, usually at rates related to configurational similarities, while other enzymes are specific for one polyol with little or no reaction with other polyols (cf. Touster & Shaw, 1962 ; Arsenis et al, 1968 ; Morton et al, 1985). The extent to which the seven different polyol dehydrogenases apparently present in P. graminis show cross-specificities can only be determined after their individual purification, although the rapid loss of activity of some of these enzymes after gel filtration may make their purification difficult.…”
Section: D-mannit01mentioning
confidence: 99%