Mannitol transport by vacuoles of storage parenchyma of celery petioles operates by facilitated diffusion

Greutert, Helen; Martinoia, Enrico; Keller, Fellx

doi:10.1016/s0176-1617(98)80050-3

Cited by 13 publications

(11 citation statements)

References 22 publications

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“…Considered as a quality parameter, the content of free sugars was analysed for fructose, glucose and mannitol. Mannitol (an alditol) was investigated because it is regarded as one of the main hydrosoluble carbohydrates in the celery petiole parenchyma 26, 27. The sucrose content was found to be below the detection threshold.…”

Section: Resultsmentioning

confidence: 99%

Texture changes in fresh cut celery during refrigerated storage

Viña

Chaves

2003

J Sci Food Agric

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The effect of refrigerated storage temperature (0 or 10 • C) on variations in texture and lignin content of minimally processed celery was assessed. In addition, the evolution of soluble phenols was studied, as well as changes in other quality parameters, namely weight loss, surface colour and contents of chlorophyll and free sugars. To this end, celery cuts were packaged on PVC film-covered polystyrene trays. The maximum shear force required to cut the strips transversally followed a similar tendency during storage at both temperatures tested. Increases in lignin content were observed which could be related to corresponding texture increases during the first few days in cold storage. There was no evidence that lignification occurred by a cicatrix-forming process in the wounds caused during cutting.

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Section: Resultsmentioning

confidence: 99%

Texture changes in fresh cut celery during refrigerated storage

Viña

Chaves

2003

J Sci Food Agric

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“…Also, in storage parenchyma discs of celery leaves and in plasma membrane vesicles from parenchyma cells, K m values of 1 mM were obtained (Keller 1991, Salmon et al 1995. In contrast, mannitol transport in vacuoles of celery parenchyma cells seems to be mediated by facilitated diffusion, because it was neither stimulated by energization with ATP and pyrophosphate, nor impaired by the dissipation of the proton-motive force (Greutert et al 1998).…”

Section: Discussionmentioning

confidence: 96%

Utilization and Transport of Mannitol in Olea europaea and Implications for Salt Stress Tolerance

Conde

Silva

Agasse

et al. 2006

Plant and Cell Physiology

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Mannitol is one of the primary photosynthetic products and the major phloem-translocated carbohydrate in Olea europaea L., an important crop in the Mediterranean basin. Uptake of mannitol in heterotrophic cell suspensions of O. europaea was shown to be mediated by a 1 : 1 polyol : H+ symport system with a Km of 1.3 mM mannitol and a Vmax of 1.3 nmol min(-1) mg(-1) DW. Dulcitol, sorbitol and xylitol competed for mannitol uptake, whereas glucose and sucrose did not. Reverse transcription-PCR (RT-PCR) performed on mRNA extracted from cultured cells exhibiting high mannitol transport activity allowed the cloning of a partial O. europaea mannitol carrier OeMaT1. The Vmax of mannitol uptake and the amount of OeMaT1 transcripts increased along with polyol depletion from the medium, suggesting that the mannitol transport system may be regulated by its own substrate. Addition of 100-500 mM NaCl to cultured cells enhanced the capacity of the polyol : H+ symport system and the amount of OeMaT1 transcripts, whereas it strongly repressed mannitol dehydrogenase activity. Measurements of cell viability showed that mannitol-grown cells remained viable 24 h after a 250 and 500 mM NaCl pulse, whereas extensive loss of cell viability was observed in sucrose-grown cells. OeMaT1 transcripts increased throughout maturation of olive fruits, suggesting that an OeMaT is involved in the accumulation of mannitol during ripening of olive. Thus, mannitol transport and compartmentation by OeMaT are important to allocate this source of carbon and energy, as well as for salt tolerance and olive ripening.

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“…Mannitol is synthesized in the cytosol of leaf cells (Rumpho et al 1983 ) and similar concentrations of mannitol in the vacuole and the cytosol indicate that diffusion of mannitol between both compartments could be responsible for the pattern observed. On the other hand, mannitol is transiently stored in the vacuoles of parenchyma cells of fleshy petioles (Greutert et al 1998 ). Similarly, Keller and Matile ( 1989 ) estimated a 3-fold higher mannitol concentration in the vacuole than in the cytosol of petiole cells, suggesting that an active uptake system of mannitol is present in the tonoplast.…”

Section: Discussionmentioning

confidence: 99%

Subcellular concentrations of sugar alcohols and sugars in relation to phloem translocation in Plantago major, Plantago maritima, Prunus persica, and Apium graveolens

Nadwodnik

Lohaus

2008

Planta

124

121

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Sugar and sugar alcohol concentrations were analyzed in subcellular compartments of mesophyll cells, in the apoplast, and in the phloem sap of leaves of Plantago major (common plantain), Plantago maritima (sea plantain), Prunus persica (peach) and Apium graveolens (celery). In addition to sucrose, common plantain, sea plantain, and peach also translocated substantial amounts of sorbitol, whereas celery translocated mannitol as well. Sucrose was always present in vacuole and cytosol of mesophyll cells, whereas sorbitol and mannitol were found in vacuole, stroma, and cytosol in all cases except for sea plantain. The concentration of sorbitol, mannitol and sucrose in phloem sap was 2-to 40-fold higher than that in the cytosol of mesophyll cells. Apoplastic carbohydrate concentrations in all species tested were in the low millimolar range versus high millimolar concentrations in symplastic compartments. Therefore, the concentration ratios between the apoplast and the phloem were very strong, ranging between 20-to 100-fold for sorbitol and mannitol, and between 200-and 2000-fold for sucrose. The woody species, peach, showed the smallest concentration ratios between the cytosol of mesophyll cells and the phloem as well as between the apoplast and the phloem, suggesting a mixture of apoplastic and symplastic phloem loading, in contrast to the herbal plant species (common plantain, sea plantain, celery) which likely exhibit an active loading mode for sorbitol and mannitol as well as sucrose from the apoplast into the phloem.

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Mannitol transport by vacuoles of storage parenchyma of celery petioles operates by facilitated diffusion

Cited by 13 publications

References 22 publications

Texture changes in fresh cut celery during refrigerated storage

Texture changes in fresh cut celery during refrigerated storage

Utilization and Transport of Mannitol in Olea europaea and Implications for Salt Stress Tolerance

Subcellular concentrations of sugar alcohols and sugars in relation to phloem translocation in Plantago major, Plantago maritima, Prunus persica, and Apium graveolens

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