Mannose-6-Phosphate Receptors as a Molecular Indicator of Maturation of Epididymal Sperm

Belmonte, Silvina; Romano, Patricia Silvia; Sosa, Miguel A. Gama

doi:10.1080/014850102753385215

Cited by 6 publications

(6 citation statements)

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“…It is interesting to note that those are common mechanisms between maturation and capacitation events [7]. Among them, the movement of some enzymes [8, 9], membrane lipids [10], and cholesterol [11] can be cited. In addition, changes in membrane fluidity as a consequence of cholesterol efflux and protein dissociation from raft domain seems to be also a mechanism that occurs both in capacitation [12, 13] and in the maturational processes occurring at the time of ejaculation [14].…”

Section: Introductionmentioning

confidence: 99%

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Caballero

Frenette

Sullivan

2011

Veterinary Medicine International

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After spermatogenesis, testicular spermatozoa are not able to fertilize an oocyte, they must undergo sequential maturational processes. Part of these essential processes occurs during the transit of the spermatozoa through the male reproductive tract. Since the sperm become silent in terms of translation and transcription at the testicular level, all the maturational changes that take place on them are dependent on the interaction of spermatozoa with epididymal and accessory gland fluids. During the last decades, reproductive biotechnologies applied to bovine species have advanced significantly. The knowledge of the bull reproductive physiology is really important for the improvement of these techniques and the development of new ones. This paper focuses on the importance of the sperm interaction with the male reproductive fluids to acquire the fertilizing ability, with special attention to the role of the membranous vesicles present in those fluids and the recent mechanisms of protein acquisition during sperm maturation.

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Section: Introductionmentioning

confidence: 99%

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Caballero

Frenette

Sullivan

2011

Veterinary Medicine International

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“…Herein, we have also observed that part of β‐NAG and, at lesser extent, β‐Gal and β‐Glu are released from the spermatozoa by treatment with M6P, suggesting some participation of mannose‐6‐phosphate receptors (MPRs) in the interaction of these enzymes with spermatozoa. It is well known that MPRs play a role in the delivery of acid hydrolases, and that two distinct MPRs occur in mammalian cells, the cation dependent‐(CD‐MPR) and the CI‐MPR receptor [Ghosh et al, ; Dahms et al, ; Braulke and Bonifacino, ], which co‐exist in most cell types, including rat spermatozoa [Belmonte et al, , ]. In line with this, the two MPRs are found in bovine spermatozoa, being the CI‐MPR highly expressed on the spermatozoa surface (Fig.…”

Section: Discussionmentioning

confidence: 84%

“…One possibility is that the binding to MPRs could be a step in the activation process of the enzymes to remodelate the sperm surface, although the epididymal pH is not the optimum for this activity. It is also possible that MPRs on sperm surface are the vehicle for these enzymes to be activated in other environment, such as the female reproductive tract [Belmonte et al, ]. However, a role for MPRs in the interaction sperm‐oocyte should not be ruled out.…”

Section: Discussionmentioning

confidence: 99%

“…Among the proteins secreted into the lumen, glycosidases can be found at high concentrations in several mammalian species [Mayorga and Bertini, ; Gupta and Setty, ; Tulsiani et al, ; Belmonte et al, ; Hermo and Robaire, ; Dacheux et al, ; Tulsiani and Abou‐Haila, ], although the role of these enzymes in sperm maturation is still controversial, since the intraluminal pH is not optimum for their catalytic activity [Levine and Kelly, ]. However, it has been demonstrated that some enzymes, such as β‐galactosidase is active in the fluid of rat epididymis [Tulsiani et al, ].…”

mentioning

confidence: 99%

“…Interestingly, MPRs have also been found in the surface of epididymal spermatozoa in some mammalian species [Belmonte et al, , ] and they could interact with enzymes secreted by the epididymal epithelium [Belmonte et al, ].…”

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confidence: 99%

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Glycosidases Interact Selectively With Mannose‐6‐Phosphate Receptors of Bull Spermatozoa

Aguilera

Boschin

Carvelli

et al. 2016

J of Cellular Biochemistry

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Glycosidases may play a role in sperm maturation during epididymal transit. In this work, we describe the interaction of these enzymes with bull spermatozoa. We found that β-galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N-acetyl-β-D-glucosaminidase and β-glucuronidase with spermatozoa appeared to be stronger. On the other hand, α-mannosidase and α-fucosidase cannot be removed from the gametes. In addition, part of N-acetyl-β-D-glucosaminidase, β-galactosidase, and β-glucuronidase can also be released by mannose-6-phosphate. Taking into account these data, we explored the presence of cation-independent- and cation-dependent-mannose-6-phosphate receptors in the spermatozoa and found that cation-independent mannose-6-phosphate receptor is highly expressed in bull spermatozoa and cation-dependent-mannose-6-phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation-independent-mannose-6-phosphate receptor is mostly located at the acrosomal zone, whereas cation-dependent-mannose-6-phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N-acetyl-β-D-glucosaminidase and β-glucuronidase isolated from epididymal fluid interacted mostly with cation-independent-mannose-6-phosphate receptor, while β-galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos-6-phosphate receptors may act as recruiters of some enzymes. J. Cell. Biochem. 117: 2464-2472, 2016. © 2016 Wiley Periodicals, Inc.

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Genomic landscape of developing male germ cells

Lee

Pang

Rennert

et al. 2009

Birth Defects Research Pt C

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Spermatogenesis is a highly orchestrated developmental process by which spermatogonia develop into mature spermatozoa. This process involves many testis-or male germ cell-specific gene products whose expressions are strictly regulated. In the past decade the advent of high-throughput gene expression analytical techniques has made functional genomic studies of this process, particularly in model animals such as mice and rats, feasible and practical. These studies have just begun to reveal the complexity of the genomic landscape of the developing male germ cells. Over 50% of the mouse and rat genome are expressed during testicular development. Among transcripts present in germ cells, 40% -60% are uncharacterized. A number of genes, and consequently their associated biological pathways, are differentially expressed at different stages of spermatogenesis. Developing male germ cells present a rich repertoire of genetic processes. Tissue-specific as well as spermatogenesis stagespecific alternative splicing of genes exemplifies the complexity of genome expression. In addition to this layer of control, discoveries of abundant presence of antisense transcripts, expressed psuedogenes, non-coding RNAs (ncRNA) including long ncRNAs, microRNAs (miRNAs) and Piwiinteracting RNAs (piRNAs), and retrogenes all point to the presence of multiple layers of expression and functional regulation in male germ cells. It is anticipated that application of systems biology approaches will further our understanding of the regulatory mechanism of spermatogenesis. †

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Mannose-6-Phosphate Receptors as a Molecular Indicator of Maturation of Epididymal Sperm

Cited by 6 publications

References 82 publications

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Post Testicular Sperm Maturational Changes in the Bull: Important Role of the Epididymosomes and Prostasomes

Glycosidases Interact Selectively With Mannose‐6‐Phosphate Receptors of Bull Spermatozoa

Genomic landscape of developing male germ cells

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