Mannose-binding lectin in term newborns and their mothers: Genotypic and phenotypic relationship

Oudshoorn, Anne-Mieke J.; Dungen, Frank A. M. van den; Bach, Kitty P.; Koomen, I.; Fetter, W. P. F.; Catsburg, Arnold; Savelkoul, Paul H. M.; Elburg, Ruurd M. van

doi:10.1016/j.humimm.2008.04.010

Cited by 17 publications

(13 citation statements)

References 29 publications

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“…As expected, based on other studies in neonates [14,20,21], ) we found a correlation between exon 1 variants and serum levels. However, the high variability in MBL serum level within a given genotype in neonates [20] and the possible influence of prematurity on MBL synthesis [13] has led to the conclusion that measurement of MBL levels reflects MBL deficiency better than MBL2 genotyping.…”

Section: Discussionsupporting

confidence: 92%

Role of mannose-binding lectin in nosocomial sepsis in critically ill neonates

et al. 2010

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Section: Discussionsupporting

confidence: 92%

Role of mannose-binding lectin in nosocomial sepsis in critically ill neonates

et al. 2010

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“…The increase in MBL is most likely of fetal origin since mRNA for the lectin is present in amniotic fluids (23), and MBL is expressed by first trimester cytotrophoblasts and mesenchymal cells (13). Recently, Oudshoorn et al reported that MBL amounts were equal in umbilical venous and arterial blood and that the levels in cord blood differ from those in maternal peripheral blood (31). Likewise, in the present study, only a weak correlation was observed in MBL levels between peripheral and cord blood (Pearson correlation coefficient ϭ 0.296).…”

Section: Discussionmentioning

confidence: 99%

Genetic Polymorphisms of Mannose-Binding Lectin Do Not Influence Placental Malaria but Are Associated with Preterm Deliveries

et al. 2009

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During pregnancy, Plasmodium falciparum-infected erythrocytes (IE) sequester in the placenta where they induce pathology and increase the risk of low-birth-weight (LBW) babies. The innate immune mediator, mannose-binding lectin (MBL), enhances phagocytosis of pathogens. Since MBL is reported to bind to IE, we hypothesized that it might aid in clearance of IE from the placenta, thereby reducing the risk of LBW babies. To test this hypothesis, molecular genotyping was used to detect polymorphisms at codon 57 (A/C) in exon 1 of MBL2 in 401 pregnant Cameroonian women, with or without placental malaria, who had LBW and normal-weight babies. Polymorphisms in the promoter region at positions ؊550 (H/L), ؊221 (X/Y), and ؉4 (P/Q) were also determined, and plasma MBL levels were measured during pregnancy and at delivery. The expected correlation between genotype and plasma MBL levels was confirmed. However, asymptomatic infections were not associated with an increase in MBL levels in the peripheral blood, and MBL levels were similar in the placental and cord blood of women with or without placental malaria at delivery. There was no evidence that MBL levels at delivery were associated with malaria-related poor pregnancy outcomes. Women with the LXPA haplotype, however, were more likely to have LBW babies, but the risk was not related to malaria. These results do not support the hypothesis that MBL aids in the clearance of parasites from the placenta but suggest that Cameroonian women with LXPA are at risk of having LBW babies due to other causes.Women who become infected with Plasmodium falciparum during pregnancy are at an increased risk of anemia and poor pregnancy outcomes (5, 25). One reason for these complications is that P. falciparum-infected erythrocytes (IE) sequester in the intervillous space (IVS) of the placenta (9). As a result, monocytes and macrophages are attracted to the IVS (30, 36), where they stimulate an inflammatory response, produce placental pathology (42), and increase the risk of low-birth-weight (LBW) babies due to intrauterine growth reduction and premature deliveries (reviewed in reference 6). Thus, elimination of IE from the IVS is of great importance.Little is known about the role of innate immune cells and factors in eliminating IE from the IVS. An acute-phase protein that might be important is mannose-binding lectin (MBL), since it promotes opsonophagocytosis and activates the MBL pathway upon binding to oligosaccharides on pathogens (12,17). MBL is reported to bind to the surface of IE and possibly merozoites, although the ligand involved remains undefined (10, 15). In addition, MBL2 genetic deficiencies in children are associated with severe malaria (4, 11). Therefore, it is possible that MBL aids in eliminating IE by enhancing phagocytosis, leading to reduced pathology and risk of LBW babies.Plasma levels of MBL are influenced by three functional single nucleotide polymorphisms (SNPs) in exon 1 of the MBL2 gene at codons 52, 54, and 57 (D, B, and C, respectively) (18,20,21,35). These SNP...

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“…The H/L variants have minimal influence on MBL concentrations. The expression pattern of the MBL protein in serum, according to the haplotypes, is established as: HYA > LYA > LXA > > HYD = LYB = LYC 17., 18.…”

Section: Introductionmentioning

confidence: 99%

Mannose-binding lectin 2 (MBL2) gene polymorphisms do not influence frequency of infections in chronic lymphocytic leukemia patients

Holanda

Lucena-Araújo

Quintas

et al. 2014

Revista Brasileira de Hematologia e Hemoterapia

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BackgroundInfectious complications represent the main cause of morbidity and mortality in chronic lymphocytic leukemia. It has been reported that polymorphisms of the mannose-binding lectin 2 (MBL2) genes are correlated with MBL protein serum levels and, consequently, are associated with the development of infectious diseases.ObjectiveThe purpose of this study was to investigate the possible association between MBL2 gene polymorphisms and risk of infection in chronic lymphocytic leukemia patients.MethodsPeripheral blood samples from 116 chronic lymphocytic leukemia patients were collected; after genomic DNA extraction, real time polymerase chain reaction was used to determine the polymorphisms of the promoter region and exon 1 of the MBL2 gene.ResultsA high frequency of Binet stage A (p-value = 0.005) and absence of splenomegaly (p-value = 0.002) were observed in patients with no infection; however, variant alleles/ genotypes and haplotypes of this gene had no impact on the risk of infection.ConclusionTo the authors’ knowledge, this is the first study describing the association between MBL2 polymorphisms and infectious disease in chronic lymphocytic leukemia. Although it was not possible to demonstrate any influence of MBL2 polymorphisms as a genetic modulator of infection in chronic lymphocytic leukemia, the authors believe that the present data are clinically relevant and provide the basis for future studies.© 2014 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. All rights reserved.

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Mannose-binding lectin in term newborns and their mothers: Genotypic and phenotypic relationship

Cited by 17 publications

References 29 publications

Role of mannose-binding lectin in nosocomial sepsis in critically ill neonates

Role of mannose-binding lectin in nosocomial sepsis in critically ill neonates

Genetic Polymorphisms of Mannose-Binding Lectin Do Not Influence Placental Malaria but Are Associated with Preterm Deliveries

Mannose-binding lectin 2 (MBL2) gene polymorphisms do not influence frequency of infections in chronic lymphocytic leukemia patients

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