AbstractsDrop-on-demand (DOD) micro-droplet formation and deposition play an important role in additive manufacturing, particularly in printing of 3D in vitro biological models for pharmacological and pathological studies, for tissue engineering and regenerative medicine applications, and for building of cell-integrated micro-fluidic devices. In development of a DOD based micro-droplet deposition process for 3D cell printing, the droplet formation, controlled on-demand deposition and at the single cell level, and most importantly, maintaining the viability and functionality of the cells during and after the printing, are all remaining to be challenged. This report presents our recent study on developing a novel DOD based micro-droplet deposition process for 3D Printing by utilization of an alternating viscous & inertial force jetting mechanism. The results include an analysis of droplet formation mechanism, the system configuration, and experimental study of the effects of process parameters on micro-droplet formation. Sodium Alginate solutions are used for micro-droplet formation and deposition. Key process parameters include actuation signal waveforms, nozzle dimensional features, and solution SUN
A c c e p t e d M a n u s c r i p t N o t C o p y e d i t e dviscosity. Sizes of formed micro-droplets are examined by measuring the droplet diameter and velocity. Resultsshow that by utilizing a nozzle at a 45μm diameter, the size of the formed micro-droplets are in the range of 52~72μm in diameter and 0.4~2.0m/s in jetting speed, respectively. Reproducibility of the system is also examined and the results show that the deviation of the formed micro-droplet diameter and the droplet deposition accuracy are within 6% and 6.2μm range, respectively. Experimental results demonstrate a high controllability and precision for the developed DOD micro-droplet deposition system with a potential for precise cell printing.