Manufacturing of nanoliposomal extract from Sargassum boveanum algae and investigating its release behavior and antioxidant activity

Abstract

In this paper, the fabrication of algal extract-loaded nanoliposomes was optimized based on the central composite response surface design. Different concentrations of phenolic compounds (500, 1,000, and 1,500 ppm) of algal extract and lecithin (0.5, 1.25, and 2% w/w) were applied for preparation of nanoliposomes at process temperatures of 30, 50, and 70°C. Dependent variables were zeta potential, entrapment efficiency, size, and particle size distribution. The particle size of the loaded nanoliposomes ranged from 86.6 to 118.7 nm and zeta potential from −37.3 to −50.7 mV.The optimal conditions were as follows: 0.5% lecithin, 30°C process temperature, and 1,313 ppm of the phenolic compounds extracted from algae. Under these conditions, the experimental entrapment efficiency of the phenolic compounds was 45.5 ± 1.2%. FTIR analysis has verified the encapsulation of algal extract in nanoliposomes. Algal extract phenolic compounds also increased phase transition temperature (Tc) of nanoliposomes (1.6°C to 6.3°C). Moreover, the thermo-oxidative protection of nanoliposomes for the algal extract has been proved by examining the DSC thermograms.It has been demonstrated that the formulated nanoliposomes have a good stability during storage conditions, and they are able to control the release of phenolic compounds at different pH values. During the encapsulation process, the antioxidant activity of the algal extract has been maintained to an acceptable level. Consequently, algal extract-loaded nanoliposomes can be used as a natural antioxidant in lipidbased foods. K E Y W O R D Salgal extract, antioxidant, encapsulation, nanoliposome, polyphenols 300 |

Available technologies on improving the stability of polyphenols in food processing

“…Savaghebi, Barzegar, and Mozafari (2020) encapsulated phenolic compounds of algal origin in nanoliposomes, after optimizing the process. Under ideal conditions, they report that thermotolerance of the compounds increased, because oxidation of free compounds began at 132.3°C, whereas for compounds that were encapsulated oxidation began at 157.1°C, according to differential scanning calorimetry.…”

Antidiabetic effect of fucoxanthin extracted from Sargassumangustifolium on streptozotocin‐nicotinamide‐induced type 2 diabetic mice

“…In recent years, marine algae in particular brown seaweeds have been documented to possess health beneficial effect because of wide range of bioactive components such as catechins, phlorotannins, flavonoids, flavonols, and flavonol glycosides (Savaghebi et al., 2020) with antioxidant, anti‐inflammatory, antiobesity, and anticarcinogenic activities (S.‐y. Kang et al, 2018; Liu et al., 2020).…”

Ocimum basilicum (kemangi) intervention on powder and microencapsulated Spirulina platensis and its bioactive molecules

“…The encapsulation efficiency of phycocyanin was in accordance with the results of previous studies 73 , which is encapsulation using an alginate coating has an encapsulation efficiency value of 71.75%. The value of encapsulation efficiency in total phenols and flavonoids in S. platensis is effected by using liposomes or nanoliposomes in encapsulation of bioactive compounds, this is because liposome is stable at low pH and is able to withstand the time of release in the stomach, but it is less consistent in the intestine 74,75 . The encapsulation efficiency of antioxidant has been shown in previous research where antioxidant microencapsulation using the freeze drying method has an encapsulation efficiency value ranging from 73-86% 76 .…”