Manufacturing of Zinc Oxide Nanoparticle (ZnO NP)-Loaded Polyvinyl Alcohol (PVA) Nanostructured Mats Using Ginger Extract for Tissue Engineering Applications

Abstract

In this research, as an alternative to chemical and physical methods, environmentally and cost-effective antimicrobial zinc oxide nanoparticles (ZnO NP) were produced by the green synthesis method. The current study focuses on the production of ZnO NP starting from adequate precursor and Zingiber officinale aqueous root extracts (ginger). The produced ZnO NP was loaded into electrospun nanofibers at different concentrations for various tissue engineering applications such as wound dressings. The produced ZnO NPs and ZnO NP-loaded nanofibers were examined by Scanning Electron Microscopy (SEM) for morphological assessments and Fourier-transform infrared spectrum (FT-IR) for chemical assessments. The disc diffusion method was used to test the antimicrobial activity of ZnO NP and ZnO NP-loaded nanofibers against three representatives strains, Escherichia coli (Gram-negative bacteria), Staphylococcus aureus (Gram-positive bacteria), and Candida albicans (fungi) microorganisms. The strength and stretching of the produced fibers were assessed using tensile tests. Since water absorption and weight loss behaviors are very important in tissue engineering applications, swelling and degradation analyses were applied to the produced nanofibers. Finally, the MTT test was applied to analyze biocompatibility. According to the findings, ZnO NP-loaded nanofibers were successfully synthesized using a green precipitation approach and can be employed in tissue engineering applications such as wound dressing.

Molecular identification of keratinophilic fungi associated with hair scalp and antifungal activity of green‐synthesis zinc oxide nanoparticles

“…Ag NPs of thyme extract showed no cytotoxic activity less than the concentration of 3.5 ppm. Izgis et al [54] conducted a study to evaluate the survivability of cells on polyvinyl alcohol (PVA)-based patches loaded with green-synthesized ZnO NPs at concentrations of 20%, 40%, and 60%, as well as on pure PVA electrospun patches. The MTT test was employed to assess cell viability in this investigation.…”

Design of Cinnamaldehyde- and Gentamicin-Loaded Double-Layer Corneal Nanofiber Patches with Antibiofilm and Antimicrobial Effects

“…26 In this study, biocompatible and biodegradable poly(vinyl alcohol) (PVA) has been reported in the literature to promote oxygen permeability, which can be a crucial feature for corneal tissue engineering field. 27,28 On the other hand, due to its excellent transparency, gelatin (GEL) has been used in ocular tissue engineering. 29 In this study, PVA/GEL nanofibers enriched with cinnamaldehyde and gentamicin were produced with an electrospinning method aiming to develop an alternative method for bacterial keratitis treatment by designing nanofibrous layers that inhibit biofilm formation and could serve as a corneal patch.…”

“…Electrospinning is a simple, versatile nanofiber production method that allows the production of biomimetic scaffolds from natural and synthetic polymers, consisting of a large network of interconnected fibers and pores, allowing the proficient exchange of nutrients and metabolic wastes, thanks to its high porosity . In this study, biocompatible and biodegradable poly­(vinyl alcohol) (PVA) has been reported in the literature to promote oxygen permeability, which can be a crucial feature for corneal tissue engineering field. , On the other hand, due to its excellent transparency, gelatin (GEL) has been used in ocular tissue engineering …”

Effect of Zinc Oxide Incorporation on the Antibacterial, Physicochemical, and Mechanical Properties of Pit and Fissure Sealants

“…Zinc oxide nanoparticles (ZnO NPs) are antibacterial materials with superior antibacterial properties to silver nanoparticles by producing reactive oxygen species [ 19 , 20 , 21 ]. Furthermore, the biocompatibility to human gingival fibroblast cells and L929 mouse fibroblast cells of ZnO NPs in dental and medical applications has been confirmed, and ZnO NPs have been used in composite resins, endodontic sealers, and adhesive cements in dental fields [ 22 , 23 , 24 , 25 ].…”