Manuka honey and methylglyoxal increase the sensitivity of Staphylococcus aureus
 to linezolid

Hayes, G. W.; Wright, N. D.; Gardner, Sarah L; Telzrow, Calla L.; Wommack, Andrew J.; Vigueira, Patrick A.

doi:10.1111/lam.12880

Cited by 28 publications

(23 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Manuka honey is one of the most popular and extensively researched medicinal honeys [1,[28][29][30][31]. It is produced in Australia and New Zealand from a monofloral source known as the Manuka tree (Leptospermum scoparium) [28].…”

Section: Introductionmentioning

confidence: 99%

Physical characteristics and antimicrobial properties of Apis mellifera, Frieseomelitta nigra and Melipona favosa bee honeys from apiaries in Trinidad and Tobago

Brown

O’Brien

Georges

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

Background: Honey is a versatile and complex substance consisting of bioactive chemicals which vary according to many bee and environmental factors. The aim of this study was to assess the physical and antimicrobial properties of five honey samples obtained from three species of bees; two stingless bees, Frieseomelitta nigra and Melipona favosa and one stinging bee, Apis mellifera (fresh and aged honey). Samples were acquired from apiaries across Trinidad and Tobago. An artificial honey, made from sugar, was also used for comparison. Methods: Physical properties such as appearance, pH, moisture content, sugar content and specific gravity were determined. Antimicrobial activity was assessed utilizing the agar diffusion assay and comparison to a phenol equivalence. The broth microdilution test was performed to determine the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of the five honey samples against four common pathogens, including Staphylococcus aureus, Escherichia coli, Streptococcus pyogenes and Haemophilus influenzae. Results: All honey samples were acidic, with pH values ranging from 2.88 (M. favosa of Tobago) to 3.91 (fresh A. mellifera). Sugar content ranged from 66.0 to 81.6% with the highest values detected in stinging bee honeys of the A. mellifera (81.6 and 80.5°Bx). Moisture content ranged from 16.9% for aged A. mellifera honey (from Trinidad) to 32.4% for F. nigra honey (from Tobago). The MICs (2 to 16%) and MBCs (2 to 32%) of stingless bee honeys were lower than that of stinging bee and artificial honeys (16 to > 32%). Stingless bee honeys also exhibited a broad spectrum of antimicrobial activity against both Gram-positive and Gram-negative organisms with higher phenol equivalence values (4.5 to 28.6%) than the A. mellifera honeys (0 to 3.4%) against the isolates tested. M. favosa honey of Tobago displayed the greatest antimicrobial activity as indicated by the high phenol equivalence and low MIC and MBC values. Conclusions: Stingless bee honeys from Tobago showed the greatest antimicrobial activity when compared to the other honeys used in this study. M. favosa honey of Tobago showed the most potential for use as medicinal honey.

show abstract

Section: Introductionmentioning

confidence: 99%

Physical characteristics and antimicrobial properties of Apis mellifera, Frieseomelitta nigra and Melipona favosa bee honeys from apiaries in Trinidad and Tobago

Brown

O’Brien

Georges

et al. 2020

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…Honey has been reported to contain over 200 compounds, including sugars, vitamins, amino acids, minerals, enzymes, flavonoids, antioxidants and phenolic acids (Eteraf-Oskouei and Najafi, 2013;Schneider et al, 2013;Alvarez-Suarez et al, 2014;Stephens et al, 2015). Manuka honey (derived from the Leptospermum scoparium tree in New Zealand) is frequently applied in the treatment of bacterial infections (Qamar et al, 2017) and exhibits well documented antibacterial properties as a result of various phenolic compounds (Carter et al, 2016;Johnston et al, 2018) and methylglyoxal, the latter following inhibition of bacterial DNA and protein synthesis (Jervis-Bardy et al, 2011;Kilty et al, 2011;Hayes et al, 2018). At bactericidal concentrations, manuka honey has been reported to cause loss of membrane integrity in both Gram positive and negative bacteria, including Pseudomonas aeruginosa (Roberts et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Whilst the general understanding of such effects in response to manuka honey are less clear, a handful of gene expression studies support a view of virulence attenuation (Jenkins et al, 2014;Roberts et al, 2014). It must be noted, however, that manuka honey exhibits a complex mode of action that is capable of acting upon multiple cellular target sites with variable cellular responses to honey reported between different bacterial species (Jenkins and Cooper, 2012;Carter et al, 2016;Hayes et al, 2018;Johnston et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Exposure to a Manuka Honey Wound Gel Is Associated With Changes in Bacterial Virulence and Antimicrobial Susceptibility

et al. 2020

View full text Add to dashboard Cite

The use of manuka honey for the topical treatment of wounds has increased worldwide owing to its broad spectrum of activity towards bacteria in both planktonic and biofilm growth modes. Despite this, the potential consequences of bacterial exposure to manuka honey, as may occur during the treatment of chronic wounds, are not fully understood. Here, we describe changes in antimicrobial susceptibility and virulence in a panel of bacteria, including wound isolates, following repeated exposure (ten passages) to sub-inhibitory concentrations of a manuka honey based wound gel. Changes in antibiotic sensitivity above 4-fold were predominantly related to increased vancomycin sensitivity in the staphylococci. Interestingly, Staphylococcus epidermidis displayed phenotypic resistance to erythromycin following passaging, with susceptibility profiles returning to baseline in the absence of further honey exposure. Changes in susceptibility to the tested wound gel were moderate (≤ 1-fold) when compared to the respective parent strain. In sessile communities, increased biofilm eradication concentrations over 4-fold occurred in a wound isolate of Pseudomonas aeruginosa (WIBG 2.2) as evidenced by a 7-fold reduction in gentamicin sensitivity following passaging. With regards to pathogenesis, 4/8 bacteria exhibited enhanced virulence following honey wound gel exposure. In the pseudomonads and S. epidermidis, this occurred in conjunction with increased haemolysis and biofilm formation, whilst P. aeruginosa also exhibited increased pyocyanin production. Where virulence attenuation was noted in a passaged wound isolate of S. aureus (WIBG 1.6), this was concomitant to delayed coagulation and reduced haemolytic potential. Overall, passaging in the presence of a manuka honey wound gel led to changes in antimicrobial sensitivity and virulence that varied between test bacteria.

show abstract

“…16 MG has been shown to act as an antibiotic adjuvant and increases the activity of linezolid against Staphylococcus aureus. 17 A combination of honey and phage has been shown to possess a synergistic effect against the biofilm formation by Escherichia coli. 18 Antibacterial activity of MG is suggested to be mediated through the structural changes of bacterial fimbriae and flagella.…”

Section: Introductionmentioning

confidence: 99%

Potential of Methylglyoxal-Conjugated Chitosan Nanoparticles in Treatment of Fluconazole-Resistant Candida albicans Infection in a Murine Model

Khan

Younus

Allemailem

et al. 2020

IJN

View full text Add to dashboard Cite

Background: Fungal infections are becoming more prevalent and threatening because of the continuous emergence of azole-resistant fungal infections. The present study was aimed to assess the activity of free Methylglyoxal (MG) or MG-conjugated chitosan nanoparticles (MGCN) against fluconazole-resistant Candida albicans. Materials and Methods: A novel formulation of MGCN was prepared and characterized to determine their size, shape and polydispersity index. Moreover, the efficacy of fluconazole or MG or MGCN was determined against intracellular C. albicans in macrophages and the systematic candidiasis in a murine model. The safety of MG or MGCN was tested in mice by analyzing the levels of hepatic and renal toxicity parameters. Results: Candida albicans did not respond to fluconazole, even at the highest dose of 20 mg/kg, whereas MG and MGCN effectively eliminated C. albicans from the macrophages and infected mice. Mice in the group treated with MGCN at a dose of 10 mg/kg exhibited a 90% survival rate and showed the lowest fungal load in the kidney, whereas the mice treated with free MG at the same dose exhibited 50% survival rate. Moreover, the administration of MG or MGCN did not induce any liver and kidney toxicity in the treated mice. Conclusion: The findings of the present work suggest that MGCN may be proved a promising therapeutic formulation to treat azole-resistant C. albicans infections.

show abstract

Manuka honey and methylglyoxal increase the sensitivity of Staphylococcus aureus to linezolid

Cited by 28 publications

References 19 publications

Physical characteristics and antimicrobial properties of Apis mellifera, Frieseomelitta nigra and Melipona favosa bee honeys from apiaries in Trinidad and Tobago

Physical characteristics and antimicrobial properties of Apis mellifera, Frieseomelitta nigra and Melipona favosa bee honeys from apiaries in Trinidad and Tobago

Exposure to a Manuka Honey Wound Gel Is Associated With Changes in Bacterial Virulence and Antimicrobial Susceptibility

<p>Potential of Methylglyoxal-Conjugated Chitosan Nanoparticles in Treatment of Fluconazole-Resistant <em>Candida albicans</em> Infection in a Murine Model</p>

Contact Info

Product

Resources

About