Many Bacterial Species are Mitogenic for Human Blood B Lymphocytes

Banck, G; Forsgren, Arne

doi:10.1111/j.1365-3083.1978.tb00528.x

Cited by 102 publications

(37 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mitogenic activity of staphylococcal cells was previously thought to be due to the presence of protein A, as protein A-rich strains possessed high mitogenic activity, whereas strains containing no protein A were very weakly or not mitogenic [16,31] [31] with whole staphylococ cal cells was much greater than that pro duced by isolated soluble or Sepharosebound protein A. These results suggested that some other components of staphylococ ci could also possess mitogenic activity, but these authors could not detect any mitogenicity of staphylococcal PG and TA.…”

Section: Discussionmentioning

confidence: 99%

“…A number of products from various bacteria has been shown to possess mitogenic activity, includ ing lipopolysaccharides (LPS) from gram negative bacteria [reviewed in 3], PPD tu berculin from Mycobacterium tuberculosis [10], acidic polysaccharide from Serratia piscatorum [11], Listeria monocytogenes cell walls [12], streptococcal extracellular products [13], and others. Also, cells of a number of microbial species have been shown to be mitogenic, including Coryne-bacterium parvum [14], Mycoplasma pneu moniae [15], Staphylococcus aureus, Hae mophilus influenzae, Bordetella pertussis, M. tuberculosis, Neisseria catarrhalis, Neis seria gonorrhoeae, Neisseria pharyngis, Ba cillus, Streptococcus pneumoniae, Strepto coccus pyogenes, and Streptococcus group G [16].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mitogenic Responsiveness of Mouse, Rat and Human Lymphocytes to <i>Staphylococcus aureus</i> Cell Wall, Teichoic Acid, and Peptidoglycan

Dziarski

Levinson³

1980

Int Arch Allergy Immunol

View full text Add to dashboard Cite

The mitogenic activity of Staphylococcus aureus peptidoglycan (PG), teichoic acid (TA) and cell wall (CW, a PG-TA complex) for mouse, rat and human lymphocytes was determined by measuring DNA synthesis. At optimal concentrations PG was the most potent mitogen, inducing maximum stimulation of mouse spleen lymphocytes on day 2 of culture, rat spleen lymphocytes on day 3, rat lymph node cells on day 2, human peripheral blood lymphocytes on day 5, and human cord blood lymphocytes on day 4. CW was less mitogenic than PG for mouse splenocytes and human peripheral and cord blood lymphocytes, with maximum stimulation occurring on days 2, 6 and 5, respectively. CW was not mitogenic for rat lymphocytes, and TA was not mitogenic for mouse, rat and human lymphocytes. At high concentrations CW and TA were cytotoxic, as indicated by markedly reduced thymidine uptake and low viability. PG was not toxic to lymphocytes. Our results suggest that TA when bound to PG can at high concentrations mask the mitogenic activity of PG and render CW cytotoxic.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mitogenic Responsiveness of Mouse, Rat and Human Lymphocytes to <i>Staphylococcus aureus</i> Cell Wall, Teichoic Acid, and Peptidoglycan

Dziarski

Levinson³

1980

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…The Fab part is then degraded, whereas the Fc fragment is endocytosed together with the Ag. M. catarrhalis selectively binds the IgD BCR, and proliferation of human B lymphocytes follows its interaction with cell surface IgD and MHC class I molecules (17)(18)(19). In the present study, we showed that MID bound all surface-expressed IgD BCRs on B lymphocytes isolated from human peripheral blood (Fig.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Novel IgD-Binding Protein fromMoraxella catarrhalis

Forsgren

Brant

Möllenkvist

et al. 2001

The Journal of Immunology

106

View full text Add to dashboard Cite

A novel surface protein of the bacterial species Moraxella catarrhalis that displays a high affinity for IgD (MID) was solubilized in Empigen and isolated by ion exchange chromatography and gel filtration. The apparent molecular mass of monomeric MID was estimated to ∼200 kDa by SDS-PAGE. The mid gene was cloned and expressed in Escherichia coli. The complete mid nucleotide gene sequence was determined, and the deduced amino acid sequence consists of 2123 residues. The sequence of MID has no similarity to other Ig-binding proteins and differs from all previously described outer membrane proteins of M. catarrhalis. MID was found to exhibit unique Ig-binding properties. Thus, in ELISA, dot blots, and Western blots, MID bound two purified IgD myeloma proteins, four IgD myeloma sera, and finally one IgD standard serum. No binding of MID was detected to IgG, IgM, IgA, or IgE myeloma proteins. MID also bound to the surface-expressed B cell receptor IgD, but not to other membrane molecules on human PBLs. This novel Ig-binding reagent promises to be of theoretical and practical interest in immunological research.

show abstract

“…In addition, H. influenzae has the capacity to reside in tonsils (25), where the IgD binding phenotype could allow for interaction with the human BCR. In fact, a mitogenic effect of Hib on human B cells has been reported (26). This effect might parallel the one M. catarrhalis has on human PBLs (27,28) and possibly serve to increase the virulence of Hib.…”

Section: Discussionmentioning

confidence: 96%

Characterization of the IgD Binding Site of Encapsulated Haemophilus influenzae Serotype b

Samuelsson

Hallström

Forsgren

et al. 2007

The Journal of Immunology

View full text Add to dashboard Cite

Encapsulated Haemophilus influenzae is a causative agent of invasive disease, such as meningitis and septicemia. Several interactions exist between H. influenzae and the human host. H. influenzae has been reported to bind IgD in a nonimmune manner, but the responsible protein has not yet been identified. To define the binding site on IgD for H. influenzae, full-length IgD and four chimeric IgDs with interspersed IgG sequences and Ag specificity for dansyl chloride were expressed in stably transfected Chinese hamster ovary cells. The binding of recombinant IgD to a panel of encapsulated H. influenzae serotype b (Hib) and nontypeable strains were investigated using a whole cell ELISA and flow cytometry. IgD binding was detected in 50% of the encapsulated Hib strains examined, whereas nontypeable H. influenzae did not interact with IgD. Finally, mapping experiments using the chimeric IgD/IgG indicated that IgD CH1 aa 198–224 were involved in the interaction between IgD and H. influenzae. Thus, by using recombinant IgD and chimeras with defined Ag specificity, we have confirmed that Hib specifically binds IgD, and that this binding involves the IgD CH1 region.

show abstract

Many Bacterial Species are Mitogenic for Human Blood B Lymphocytes

Cited by 102 publications

References 28 publications

Mitogenic Responsiveness of Mouse, Rat and Human Lymphocytes to <i>Staphylococcus aureus</i> Cell Wall, Teichoic Acid, and Peptidoglycan

Mitogenic Responsiveness of Mouse, Rat and Human Lymphocytes to <i>Staphylococcus aureus</i> Cell Wall, Teichoic Acid, and Peptidoglycan

Isolation and Characterization of a Novel IgD-Binding Protein fromMoraxella catarrhalis

Characterization of the IgD Binding Site of Encapsulated Haemophilus influenzae Serotype b

Contact Info

Product

Resources

About