Map-based cloning of the ERECT PANICLE 3 gene in rice

Piao, Rihua; Jiang, Wenzhu; Ham, Tae-Ho; Choi, Mansoo; Qiao, Yongli; Chu, Sang-Ho; Park, Jung Hyun; Woo, Mi-Ok; Zheng-xun, Jin; An, Gynheung; Lee, Joohyun; Koh, Hee‐Jong

doi:10.1007/s00122-009-1151-x

Cited by 86 publications

(69 citation statements)

References 30 publications

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“…And the increased diameter of both culm and panicle may help to enhance the potential in lodging and fertilizer resistance. Genetic studies of erect panicle varieties derived from Balilla and other mutants have identified panicle erectness genes, such as DEP1/qPE9-1, EP2, and EP3, in recent years [10][11][12][14][15][16]. Unlike dep1, the number of spikelets and primary and secondary branches was almost the same in dep2 compared to the wild type.…”

Section: Discussionmentioning

confidence: 99%

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Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation

et al. 2010

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The architecture of the panicle, including grain size and panicle morphology, directly determines grain yield. Panicle erectness, which is selected for achieving ideal plant architecture in the northern part of China, has drawn increasing attention of rice breeders. Here, dense and erect panicle 2 (dep2) mutant, which shows a dense and erect panicle phenotype, was identified. DEP2 encodes a plant-specific protein without any known functional domain. Expression profiling of DEP2 revealed that it is highly expressed in young tissues, with most abundance in young panicles. Morphological and expression analysis indicated that mutation in DEP2 mainly affects the rapid elongation of rachis and primary and secondary branches, but does not impair the initiation or formation of panicle primordia. Further analysis suggests that decrease of panicle length in dep2 is caused by a defect in cell proliferation during the exponential elongation of panicle. Despite a more compact plant type in the dep2 mutant, no significant alteration in grain production was found between wild type and dep2 mutant. Therefore, the study of DEP2 not only strengthens our understanding of the molecular genetic basis of panicle architecture but also has important implications for rice breeding.

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Section: Discussionmentioning

confidence: 99%

“…In the case of the 'Green Revolution', grain yields have been significantly increased by growing lodging-resistant semi-dwarf varieties of wheat and rice [6,7]. have been reported; EP2 was mapped to chromosome 4, and EP3 encodes a putative F-box protein [15,16].…”

Section: Introductionmentioning

confidence: 99%

Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation

et al. 2010

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“…Paraffin-embedded peduncle tissue sections were prepared following the methods described by (Piao et al 2009) with slight modifications. Panicles were harvested at ripening stage.…”

Section: Histological Analysismentioning

confidence: 99%

“…There are several reports on genetic and molecular mechanisms controlling panicle architecture in rice such as DEP1 (dense and erect panicle 1) (Huang et al 2009), DEP2 (dense and erect panicle 2) (Li et al 2010), DEP3 (dense and erect panicle 3) (Qiao et al 2011), EP (erect pose panicle) (Wang et al 2009), EP2 (erect panicle 2) (Zhu et al 2010) and EP3 (erect panicle 3) (Piao et al 2009). DEP1 locus is a gain-of-function mutation causing truncation of a phosphatidylethanolamine-binding protein-like domain protein.…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping and Candidate Gene Analysis of Small Round Grain Mutant in Rice

Seo

Bordiya

Lee

et al. 2017

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Grain size and panicle architecture are important traits determining yield. Here we report a new allele of DEP2 (dense and erect panicle 2) which has a pleiotropic effect on grain size/shape and panicle architecture. A small round grain and erect panicle mutant was obtained by treating japonica cultivar Hwacheong waxy with MNU (N-methyl-N-nitrosourea). Through fine mapping sequence analysis, we identified the single nucleotide substitution in the mutant, which caused splicing error of the first intron, on previously reported DEP2 locus in rice. Since the dep2-1 and dep2-2 have been reported in previous studies, we named this new allele as dep2-3. The dep2-3 mutant manifested reduced grain size and plant height, dense and erect panicle and erect plant architecture. When we crossed the mutant with wild type, panicle and grain of F1 plant showed intermediate phenotype, indicating that dep2-3 showed incomplete dominance, which was not reported in the previous study. Scanning electron microscopy (SEM) results showed that increase in width of mutant grain was due to the increased width of glume cells. Paraffin section of peduncle revealed that outgrowth in outer layer of peduncle compared to inner layer caused the erectness of the panicle. Our results collectively indicated that the dep2-3 might play a significant role in the regulation of grain size/shape and formation of vascular bundles.

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“…[EP2], EP3) have also been isolated recently (Huang et al, 2009;Piao et al, 2009;Zhou et al, 2009;Zhu et al, 2010). Interestingly, these genes also affect grain shape, although they were originally isolated because of their effect on panicle traits.…”

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confidence: 99%

SHORT GRAIN1 Decreases Organ Elongation and Brassinosteroid Response in Rice

et al. 2011

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We identified a short-grain mutant (Short grain1 (Sg1) Dominant) via phenotypic screening of 13,000 rice (Oryza sativa) activation-tagged lines. The causative gene, SG1, encodes a protein with unknown function that is preferentially expressed in roots and developing panicles. Overexpression of SG1 in rice produced a phenotype with short grains and dwarfing reminiscent of brassinosteroid (BR)-deficient mutants, with wide, dark-green, and erect leaves. However, the endogenous BR level in the SG1 overexpressor (SG1:OX) plants was comparable to the wild type. SG1:OX plants were insensitive to brassinolide in the lamina inclination assay. Therefore, SG1 appears to decrease responses to BRs. Despite shorter organs in the SG1:OX plants, their cell size was not decreased in the SG1:OX plants. Therefore, SG1 decreases organ elongation by decreasing cell proliferation. In contrast to the SG1:OX plants, RNA interference knockdown plants that down-regulated SG1 and a related gene, SG1-LIKE PROTEIN1, had longer grains and internodes in rachis branches than in the wild type. Taken together, these results suggest that SG1 decreases responses to BRs and elongation of organs such as seeds and the internodes of rachis branches through decreased cellular proliferation.

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Map-based cloning of the ERECT PANICLE 3 gene in rice

Cited by 86 publications

References 30 publications

Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation

Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation

Fine Mapping and Candidate Gene Analysis of Small Round Grain Mutant in Rice

SHORT GRAIN1 Decreases Organ Elongation and Brassinosteroid Response in Rice

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