2006
DOI: 10.1016/j.mce.2006.03.015
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MAP-quest: Could we produce constitutively active variants of MAP kinases?

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Cited by 19 publications
(16 citation statements)
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“…Also, although vast information is available with respect to the structural requirements for MAPK activation (45,46), it is not known how to impose these structural changes via mutagenesis (48). We therefore took a genetic approach and were able to generate mutants of the human MAPK p38␣, which were spontaneously active as recombinant proteins expressed in and purified from Escherichia coli cells (49).…”
mentioning
confidence: 99%
“…Also, although vast information is available with respect to the structural requirements for MAPK activation (45,46), it is not known how to impose these structural changes via mutagenesis (48). We therefore took a genetic approach and were able to generate mutants of the human MAPK p38␣, which were spontaneously active as recombinant proteins expressed in and purified from Escherichia coli cells (49).…”
mentioning
confidence: 99%
“…For a very long time and despite many efforts to obtain intrinsically active variants of Erks (30,37), such proteins could not be achieved. Now that such molecules are available for yeast, flies, and mammals, novel approaches are open for investigating, in a specific manner, the biological and biochemical functions of each isoform and splicing variants of these kinases.…”
Section: Discussionmentioning
confidence: 99%
“…Also, attempts to mimic phosphorylation by replacing the relevant Thr to Glu were not successful (25,29). A large number of studies applying other strategies were also just partially successful (30). Several years ago, we took a genetic approach for isolation of intrinsically active mutants of the yeast MAPK Hog1.…”
mentioning
confidence: 99%
“…It should be noted that numeration of the mutations in ERK1 (in text and in Table 1A) refer to the sequence of the human protein and numeration of mutations in ERK2 (in text and in Table 1B) refer to the sequence of rat protein. Notably, mutations that had been discovered (until 2006) in ERK orthologs in lower organisms were summarized in [98] and will not be discussed here.…”
Section: Almost All Known Mutations In Erks Have Been Identified Expementioning
confidence: 99%