MAPK immunoreactivity in streptozotocin-induced diabetic rat testis

Abstract

PURPOSE:To evaluate the alterations of two mitogen-activated protein kinases (MAPK)s, extracellular signal regulated kinase (ERK) and c-Jun NH2 terminal kinase (JNK), in the testes of male rats with experimental diabetes. METHODS:Twenty males Sprague-Dawley rats were randomly divided into a control group (n=8) and a diabetes group (administration of 40 mg/kg/day streptozotocin (STZ) for five sequential days, n=12). After six weeks, testicular biopsy samples were obtained for light microscopy and immunohistochemical methods. RESULTS:The PCNA (proliferating cell nuclear antigen) index was significantly decreased in the diabetes group (p=0.004) when compared to the control group. Both total (t)-ERK and phosphor (p)-ERK immunoreactivities were significantly decreased in the diabetes group (p=0.004, p<0.001, respectively). The t-JNK immunoreactivity was unchanged in both groups (p=0.125), while p-JNK immunoreactivity was significantly increased in the diabetic group (p=0.002). CONCLUSIONS:The decrease of androgen levels in the course of diabetes may contribute to the decrease of the immunoreactivities of t-ERK and p-ERK. JNK may be activated due to the changes in various cytokines and chemochines that participate in the oxidative stress process of diabetes. Therefore, testicular apoptosis may occur and lead to infertility associated with diabetes.

Comparison of male reproductive parameters in mice with type 1 and type 2 diabetes

“…In T2DM mice, the prevalence was types h >g >d >f >b >c =e at 36 days, and it was types b >h >e >f >g >c >d at 72 days. The decreases in diameter and epithelial height of the seminiferous tubules of T1DM and T2DM mice in our study were similar to those found in previous reports [23,38,[42][43][44]. Our study also found a positive correlation between seminiferous tubule diameter and spermatogenesis along with decreased sperm concentration in the T1DM and T2DM mice.…”

Testicular histopathology and phosphorylated protein changes in mice with diabetes induced by multiple-low doses of streptozotocin: An experimental study

“…The observed tubular histopathology was classified into seven types ( Figure 6B-H ). The intraepithelial vacuolization found in this study is similar to that of previous studies in OHD-STZ rats ( 32 - 34 ). This type of histopathology is one of the most common injuries in Sertoli cells ( 35 ).…”

“…This type of histopathology is one of the most common injuries in Sertoli cells ( 35 ). Furthermore, the decreases in seminiferous tubule epithelium diameter found in this study were similar to those found in OHD-STZ rats ( 3 , 32 , 36 ).…”

Protective efficiency ofAshrasidate palm hydroalcoholic extract against diabetes‐induced testicular toxicity: A biochemical and stereological study

“…Earlier studies showed that hyperglycaemia increases the expression of Bax protein as a pre-apoptotic gene and causes apoptosis in PC1 cells (Sharifi, Mousavi, Farhadi, & Larijani, 2007). In the testis of diabetic rats, apoptosis is accompanied with downregulation of Bcl-2 gene expression (Donmez, Kizilay, & Topcu-Tarladacalisir, 2014;Jafari Anarkooli, Sankian, Ahmadpour, Varasteh, & Haghir, 2008). This study revealed that treatment of diabetic rats with ADP extract prevented apoptosis through upregulating the expression of Bcl-2 protein.…”

“…The present results likewise showed lover testosterone level in diabetic rats. In the testis of diabetic rats, apoptosis is accompanied with downregulation of Bcl-2 gene expression (Donmez, Kizilay, & Topcu-Tarladacalisir, 2014;Jafari Anarkooli, Sankian, Ahmadpour, Varasteh, & Haghir, 2008). In this study, the Leydig cells of diabetic animals were assessed both quantitatively using physical discetor and qualitatively for Blc-2 expression.…”