Mapping a quantitative trait locus for the concentration of β-lactoglobulin in milk, and the effect of β-lactoglobulin genetic variants on the composition of milk from Holstein-Friesian x Jersey crossbred cows

Berry, Sarah D.; López‐Villalobos, N.; Beattie, E. M.; Davis, Stephen R.; Adams, Lowell; Thomas, Natalie; Ankersmit-Udy, Alexandra E.; Stanfield, Angela M.; Lehnert, Klaus; Ward, Hamish; Arias, J.; Spelman, Richard; Snell, Russell G.

doi:10.1080/00480169.2010.65053

Cited by 20 publications

(15 citation statements)

References 21 publications

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“…Both genotypes BC and AA had a relatively low frequencies, 0.02 and 0.09, which were similar to 0.01 and 0.16 reported by Berry et al [4] for a New Zealand F1 Holstein x Jersey population used to produce milk with high percentage of total solids.…”

Section: β-Lactoglobulinsupporting

confidence: 81%

“…The frequency estimated for the C allele, 0.02, was similar to 0.01 reported by Berry et al [4] in a New Zealand herd of F1 Holstein x Jersey. The result from the present study could be explained because some of the sampled cows in the Mexican Jersey population were imported from New Zealand.…”

Section: β-Lactoglobulinsupporting

confidence: 78%

“…Particularly, the Jersey cattle populations have been constituted by American, Canadian [1], European, Australian and New Zealander progenitors. Jersey populations of these countries have been reported with variable gene frequencies for milk protein genes [2,3,4].…”

Section: Introductionmentioning

confidence: 98%

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Polymorphism of three milk protein genes in Mexican Jersey cattle

Zepeda-Batista¹,

Alarcón-Zúñiga²,

Ruíz-Flores³

et al. 2015

Electronic Journal of Biotechnology

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The objective was to estimate the allelic and genotypic frequencies, genetic diversity and polymorphic information content for the β-casein, κ-casein and β-lactoglobulin genes. Blood and frozen semen samples were collected from 453 Jersey individuals registered by the Mexican Jersey Cattle Association. Twenty eight breed specific SNP primers for whole genes were used. The B allele of κ-casein had higher frequency (0.69) than the A (0.26) and E (0.05). For β-lactoglobulin, the highest frequency was for B (0.72), followed by A and C alleles (0.26 and 0.02, respectively). The β-casein allele with the highest frequency was A 2 (0.71), followed by A 1 (0.19), A 3 (0.05), B (0.04) and C (0.01). The average genetic diversity (He) was 0.53. The average locus effective allele number was 1.79. These results indicate a high allelic diversity for κ-caseín, β-casein and β-lactoglobulin that could be included in breeding programs in the population studied, aimed to improve the milk quality traits of economic importance.

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Section: β-Lactoglobulinsupporting

confidence: 81%

Section: β-Lactoglobulinsupporting

confidence: 78%

See 1 more Smart Citation

Polymorphism of three milk protein genes in Mexican Jersey cattle

Zepeda-Batista¹,

Alarcón-Zúñiga²,

Ruíz-Flores³

et al. 2015

Electronic Journal of Biotechnology

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“…The analogous roles of AGPAT6 and DGAT1 might suggest that these pleiotropic effects on milk composition are a response peculiar to alterations in the rate of triglyceride synthesis, however we have observed similar pleiotropic effects for other major milk components. As an example, the fourth largest milk fat percentage effect estimated in our GWAS of 32,530 cows was for the 103–104 Mbp interval on chromosome 11 (Table S2), representing a region that encompasses a well-documented QTL for the major milk protein beta-lactoglobulin [21]. Taken together, these observations suggest that there are broad, interrelated regulatory mechanisms governing the yield and concentration of major milk components, and that a change in one of these components leads to a reconfiguration of total milk composition.…”

Section: Discussionmentioning

confidence: 99%

Expression Variants of the Lipogenic AGPAT6 Gene Affect Diverse Milk Composition Phenotypes in Bos taurus

et al. 2014

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Milk is composed of a complex mixture of lipids, proteins, carbohydrates and various vitamins and minerals as a source of nutrition for young mammals. The composition of milk varies between individuals, with lipid composition in particular being highly heritable. Recent reports have highlighted a region of bovine chromosome 27 harbouring variants affecting milk fat percentage and fatty acid content. We aimed to further investigate this locus in two independent cattle populations, consisting of a Holstein-Friesian x Jersey crossbreed pedigree of 711 F2 cows, and a collection of 32,530 mixed ancestry Bos taurus cows. Bayesian genome-wide association mapping using markers imputed from the Illumina BovineHD chip revealed a large quantitative trait locus (QTL) for milk fat percentage on chromosome 27, present in both populations. We also investigated a range of other milk composition phenotypes, and report additional associations at this locus for fat yield, protein percentage and yield, lactose percentage and yield, milk volume, and the proportions of numerous milk fatty acids. We then used mammary RNA sequence data from 212 lactating cows to assess the transcript abundance of genes located in the milk fat percentage QTL interval. This analysis revealed a strong eQTL for AGPAT6, demonstrating that high milk fat percentage genotype is also additively associated with increased expression of the AGPAT6 gene. Finally, we used whole genome sequence data from six F1 sires to target a panel of novel AGPAT6 locus variants for genotyping in the F2 crossbreed population. Association analysis of 58 of these variants revealed highly significant association for polymorphisms mapping to the 5′UTR exons and intron 1 of AGPAT6. Taken together, these data suggest that variants affecting the expression of AGPAT6 are causally involved in differential milk fat synthesis, with pleiotropic consequences for a diverse range of other milk components.

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“…The B variant has been associated with higher casein percentage (Lundén et al, 1997;Berry et al, 2010), higher total solids (McLean et al, 1984), and higher milk yield, fat yield, fat percentage, and lactose yield (Tsiaras et al, 2005), whereas the A variant is associated with higher whey protein content and lower casein content (Auldist et al, 2000). The B variant has been associated with higher casein percentage (Lundén et al, 1997;Berry et al, 2010), higher total solids (McLean et al, 1984), and higher milk yield, fat yield, fat percentage, and lactose yield (Tsiaras et al, 2005), whereas the A variant is associated with higher whey protein content and lower casein content (Auldist et al, 2000).…”

Section: Existing Variation In Bovine Milk Proteins and The Impact Onmentioning

confidence: 99%

Significance, Origin, and Function of Bovine Milk Proteins: The Biological Implications of Manipulation or Modification

Berry¹,

Sheehy²,

Williamson³

et al. 2014

Milk Proteins

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Mapping a quantitative trait locus for the concentration of β-lactoglobulin in milk, and the effect of β-lactoglobulin genetic variants on the composition of milk from Holstein-Friesian x Jersey crossbred cows

Cited by 20 publications

References 21 publications

Polymorphism of three milk protein genes in Mexican Jersey cattle

Polymorphism of three milk protein genes in Mexican Jersey cattle

Expression Variants of the Lipogenic AGPAT6 Gene Affect Diverse Milk Composition Phenotypes in Bos taurus

Significance, Origin, and Function of Bovine Milk Proteins: The Biological Implications of Manipulation or Modification

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