Mapping and candidate-gene screening of the novel Turnip mosaic virus resistance gene retr02 in Chinese cabbage (Brassica rapa L.)

Abstract

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The role of the 5′ untranslated regions of Potyviridae in translation

“…Recent findings revealed that the viral protein linked to the 5 end of the genome (VPg) that replaces the 5 cap and determines infectivity for many potyviruses (Ayme et al, 2006;Qian et al, 2013;Truniger and Aranda, 2009) can affect translation (Eskelin et al, 2011;Khan et al, 2008;Miyoshi et al, 2008). The addition of the TEV VPg with eIF4F to a cap-binding complex-depleted wheat germ extract enhanced TEV-mediated translation by almost 3-fold when compared to eIF4F alone.…”

Multiple copies of eukaryotic translation initiation factors in Brassica rapa facilitate redundancy, enabling diversification through variation in splicing and broad‐spectrum virus resistance

“…Sequencing of the genes and comparison of the RLR22 sequence with a sequence‐based genetic map of B. rapa (Wang et al ., ) confirmed the location of retr01 ( BraA.eIF(iso)4E.a ) on chromosome A4 in B. rapa . Molecular markers located retr02 on a scaffold also from chromosome A4 (Qian et al ., ). The location of the ConTR01 candidates BraA.eIF4E.c and BraA.eIF(iso)4E.c from RLR22 (Rusholme et al ., ) was confirmed on chromosome A8, again by comparison with the sequence‐based genetic map (Wang et al ., ).…”

Identification and mapping of a novel Turnip mosaic virus resistance gene TuRBCS01 in Chinese cabbage (Brassica rapa L.)

“…This might be due to the different susceptible parents used in the two experiments: Ji Zao Chun was used in the study by Qian et al. (), and Guan291 was used in the present study. Yoon et al.…”

“…The recessive gene retr02 was mapped to chromosome A04 of the A genome in B. rapa between two InDel markers, BrID10694 (0.3 cM) and BrID101309 (0.6 cM; Qian et al. ). Considering the rich Brassica TuMV resistance resources, the number of mapped TuMV resistance genes is relatively small.…”