Mapping and characterization of novel parthenocarpy QTLs in tomato

Gorguet, Benoît; Eggink, P.M.; Ocaña, Juan; Tiwari, Aparna; Schipper, Danny; Finkers, Richard; Visser, Richard G. F.; Heusden, A.W. van

doi:10.1007/s00122-007-0708-9

Cited by 64 publications

(56 citation statements)

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“…For example, Chen and Tanksley (2004) identified 123 recombinants from 1,535 F2 individuals within the S. pennellii IL 2-5 region for the fine-mapping of se2.1 on chromosome 2. Recombination suppression varies across Solanaceae genomes and chromosomal locations (Alpert et al, 1995;Ku et al, 1999;Monforte and Tanksley, 2000;Gorguet et al, 2008), and in some regions, recombination hotspots have been identified, including on S. pennellii chromosome 9 (Fridman et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…Wild tomato species offer a rich and largely unexplored source of new genetic variation for breeders. A number of studies of tomato traits including fruit size (Frary et al, 2000;Cong et al, 2008), Brix (Fridman et al, 2004), parthenocarpy (Gorguet et al, 2008), and the evolutionary transition from allogamous flowers to autogamous flowers (Chen and Tanksley, 2004) have utilized wild tomato species ILs to identify genes involved in these processes. In this study, the 76 Solanum pennellii introgression lines in a S. lycopersicum background (cv M82; Eshed and Zamir, 1994) were used to identify QTLs for the textural properties.…”

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confidence: 99%

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High-Resolution Mapping of a Fruit Firmness-Related Quantitative Trait Locus in Tomato Reveals Epistatic Interactions Associated with a Complex Combinatorial Locus

Chapman

Bonnet²,

Grivet³

et al. 2012

Plant Physiology

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Fruit firmness in tomato (Solanum lycopersicum) is determined by a number of factors including cell wall structure, turgor, and cuticle properties. Firmness is a complex polygenic trait involving the coregulation of many genes and has proved especially challenging to unravel. In this study, a quantitative trait locus (QTL) for fruit firmness was mapped to tomato chromosome 2 using the Zamir Solanum pennellii interspecific introgression lines (ILs) and fine-mapped in a population consisting of 7,500 F2 and F3 lines from IL 2-3 and IL 2-4. This firmness QTL contained five distinct subpeaks, Fir s.p. QTL2.1 to Fir s.p. QTL2.5, and an effect on a distal region of IL 2-4 that was nonoverlapping with IL 2-3. All these effects were located within an 8.6-Mb region. Using genetic markers, each subpeak within this combinatorial locus was mapped to a physical location within the genome, and an ethylene response factor (ERF) underlying Fir s.p. QTL2.2 and a region containing three pectin methylesterase (PME) genes underlying Fir s.p. QTL2.5 were nominated as QTL candidate genes. Statistical models used to explain the observed variability between lines indicated that these candidates and the nonoverlapping portion of IL 2-4 were sufficient to account for the majority of the fruit firmness effects. Quantitative reverse transcription-polymerase chain reaction was used to quantify the expression of each candidate gene. ERF showed increased expression associated with soft fruit texture in the mapping population. In contrast, PME expression was tightly linked with firm fruit texture. Analysis of a range of recombinant lines revealed evidence for an epistatic interaction that was associated with this combinatorial locus.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

High-Resolution Mapping of a Fruit Firmness-Related Quantitative Trait Locus in Tomato Reveals Epistatic Interactions Associated with a Complex Combinatorial Locus

Chapman

Bonnet²,

Grivet³

et al. 2012

Plant Physiology

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“…However, all F 3 plants that were homozygous for the 'MPK-1' allele exhibited strong parthenocarpy, indicating that the major gene that causes parthenocarpy in 'MPK-1' is located near TGS0486. It has previously been reported that pat is localized on the long arm of chromosome 3; pat-2, pat4.1, and pat4.2 are localized on chromosome 4; pat5.1 is localized on chromosome 5; and pat9.1 is localized on chromosome 9 (Beraldi et al, 2004;Gorguet et al, 2008;Nunome et al, 2013). Therefore, this is the first report of a parthenocarpic gene on chromosome 1 in the tomato.…”

mentioning

confidence: 95%

“…In the tomato, this trait is considered attractive as it reduces the financial and labor costs of fruit setting. There are currently five parthenocarpic tomato resources that are controlled by eight different parthenocarpic genes-'Soressi' and 'Montfavet191' (pat), 'Severianin' (pat-2), 'RP75/59' (pat3/pat4), 'IL5-1' (pat4.1/pat5.1), and 'IVT-line1' (pat4.2/pat9.1) (Gorguet et al, 2005(Gorguet et al, , 2008. Genetic linkage maps have been constructed for five of these genes, pat, pat4.1, pat4.2, pat5.1, and pat9.1 (Beraldi et al, 2004;Gorguet et al, 2008), and pat-2 was recently mapped to chromosome 4 and found to encode a zinc finger homeodomain protein (Nunome et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Parthenocarpy in the Tomato (<i>Solanum lycopersicum</i> L.) Cultivar ‘MPK-1’ is Controlled by a Novel Parthenocarpic Gene

Takisawa¹,

Maruyama²,

Nakazaki³

et al. 2017

Hortic J

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Parthenocarpy is a trait where fruit set and growth are triggered without pollination and fertilization. In the tomato (Solanum lycopersicum L.), this trait is considered attractive as it reduces the cost and labor requirements for fruit setting. In this study, we investigated the inheritance of parthenocarpy in 'MPK-1'-a parthenocarpic tomato cultivar derived from a cross between a variant from a self-fertilization posterity of 'Severianin', which exhibited strong parthenocarpy and a non-parthenocarpic cultivar. It was reported that 'MPK-1' contains a pat-2 gene because 'Severianin' which has a pat-2 gene is its only parthenocarpic ancestor. However, we found that parthenocarpy in 'MPK-1' is controlled by a novel parthenocarpic gene, not pat-2. This novel gene, which was designated as Pat-k, is semi-dominant and located on chromosome 1. We also showed that the size of the parthenocarpic fruit of 'MPK-1' is similar to that of the pollinated fruit at maturity. Thus, 'MPK-1' may be used as a new parthenocarpic resource for breeding.

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“…Estas líneas han sido las más estudiadas pero también existen otros genotipos con cierta tendencia a desarrollar frutos sin semillas como: PI 190256, Oregon Cherry, Oregon 11, Carobeta (Spena y Rotino, 2001) y las líneas de introgresión de Solanum habrochaites (S. Knapp y D.M. Spooner) IVTline1 y IL51 (Gorguet et al, 2008). Sin embargo, a pesar de la existencia de diferentes fuentes naturales de partenocarpia, aún no se ha conseguido aislar ninguno de los genes responsables de este fenotipo.…”

Section: Fuentes Naturales De Partenocarpia En Tomateunclassified

Análisis del cuajado y desarrollo partenocárpico del fruto en solanáceas: identificación de genes implicados

Bañuls¹

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AGRADECIMIENTOSCuando miro hacia atrás me sorprendo de haber llegado hasta aquí, y me doy cuenta de que no lo habría conseguido sin el apoyo de mucha gente que ha estado ahí para ayudar y a la que tengo mucho que agradecer.En primer lugar querría agradecer a mis directores de tesis la dedicación que me han prestado. Al Dr. Fernando Nuez quiero agradecerle la oportunidad que me brindó de trabajar en el COMAV y de desarrollar esta tesis. Al Dr. Joaquín Cañizares le estoy muy agradecida por haber estado ahí en todo momento, para enseñar y aconsejar, sin su apoyo no habría llegado hasta aquí.Me gustaría agradecer todo el apoyo que he recibido de la gente del departamento de Bioinformática del CIPF, a Ana Conesa y a Stefan Goetz por su ayuda con la anotación del los microarrays, y a Fatima Al-Shahrour por implantar nuestra anotación en el Babelomics. También me gustaría agradecer a Montse Plomer y María García su ayuda el el invernadero, y a Jose Blanca su apoyo con toda la parte bioinformática.Además hay un montón de amigos y compañeros del COMAV que en un momento u otro me han ayudado con dudas varias o simplemente estando ahí para hablar y compartir los momentos más duros. Muchas gracias a Elena, Laura C, Laura A, Montse y Toya con las que he tenido el privilegio de compartir parte del camino. También a todos los que siguen por aquí dando guerra Pascual, Inma, Peio, Cris, Carmelo, Ana P, Nuria, Mariola, Javi, Alicia, Santi, Ana A... Para no dejarme a nadie mil gracias a todos.Muchas gracias a toda mi familia, por aguantar lo peor de los nervios, en especial a mi madre y Oti por todas las fiambreras sin las que no habría sobrevivido. Y por supuesto muchas gracias a Robert por apoyarme en todo momento y creer en mi mas que yo.A mi abuela Amparo RESUMEN El cuajado del fruto es uno de los procesos más importantes del desarrollo vegetal, de él dependen la reproducción y propagación de la planta, cuando se trata de especies silvestres, y la producción en el caso de las especies cultivadas. El estudio de los genes regulados durante este proceso es crucial para comprender mejor los mecanismos implicados y mejorar el cuajado, especialmente en condiciones ambientales adversas. El tomate, Solanum lycopersicum, además de ser uno de los principales cultivos de hortalizas, ha sido ampliamente utilizado en investigación, tanto básica como aplicada. Sin embargo, la mayoría de estudios del desarrollo del fruto en esta especie se han centrado en los procesos que tienen lugar durante la maduración y no han abordado el estudio del cuajado a gran escala. En este sentido, el estudio de materiales con capacidad partenocárpica tiene especial interés, facilita el estudio del cuajado sin interferencia de los procesos relacionados con el desarrollo del embrión y posibilita el estudio de los estadios tempranos del desarrollo del fruto independientemente de la polinización. Además, el empleo de estos materiales en la mejora es especialmente ventajoso puesto que permite obtener frutos incluso en ausencia de polinización y fecundación. En tomat...

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Mapping and characterization of novel parthenocarpy QTLs in tomato

Cited by 64 publications

References 22 publications

High-Resolution Mapping of a Fruit Firmness-Related Quantitative Trait Locus in Tomato Reveals Epistatic Interactions Associated with a Complex Combinatorial Locus

High-Resolution Mapping of a Fruit Firmness-Related Quantitative Trait Locus in Tomato Reveals Epistatic Interactions Associated with a Complex Combinatorial Locus

Parthenocarpy in the Tomato (<i>Solanum lycopersicum</i> L.) Cultivar ‘MPK-1’ is Controlled by a Novel Parthenocarpic Gene

Análisis del cuajado y desarrollo partenocárpico del fruto en solanáceas: identificación de genes implicados

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