Mapping and CRISPR homology-directed repair of a recessive white eye mutation in Plodia moths

Efficienthyperactive piggyBactransgenesis inPlodiapantry moths

“…We then co-injected the donor plasmid pBac[3xP3::EGFP; nosO::mScarlet] with a hyPBase mRNA and monitored somatic transformation efficiencies throughout the G 0 generation. In order to facilitate the screening of fluorescence, all experiments were performed in the Pi_wFog white-eyed strain that is devoid of screening pigments in eye tissues and also shows increased larval translucency (Heryanto et al, 2022).Transformed embryos and first instar hatchlings showed ocellar and glial EGFP fluorescence (Fig. 2C-D), with 23.7% of injected eggs showing EGFP in 72-h embryos (Table 1).…”

“…The resulting hybrid Pi_wFog strain has been maintained in inbred state for 3 years and used throughout this study. All rearing used previously published methods (Heryanto et al, 2022), using special containers and a wheat bran-sucrose-glycerol diet (Silhacek and Miller, 1972). A rearing temperature of 28°C resulted in a generation time of 28 d.…”

“…Donor plasmids without hyPBase mRNA were injected in separate experiments as controls. Microinjection procedures (Figure 1A) followed a previously described procedure (Heryanto et al, 2022), with all embryo injections performed within 40 min after egg laying (AEL). Injected embryos were counted and kept in a rearing container with a small damp Kimwipe at 28°C.…”

“…emergence, after which they were transferred to a vented rearing container with a bed of Plodia food (modified LocknLock containers described in Heryanto et al, 2022;177 mL and 350 mL formats). At the wandering L5 stage, cardboard "hotels" were added into the containers for pupal isolation.…”

Effect of behavioral conditions on silk characteristics in the Indian meal moth (Plodia interpunctella)

“…Other lepidopterans, such as the luna moth (Actias luna, Linnaeus, Saturniidae; Chen et al, 2012;Reddy and Yang, 2012) and the Indian meal moth (Plodia interpunctella, Hübner, Pyralidae; Milutinović et al, 2020;Shim and Lee, 2015) are now being studied as the search for tougher, stronger, or more flexible silks (Yoshioka et al, 2019). While recent studies on P. interpunctella have focused on it as a model organism for genomic study of silk production and evolution (Kawahara et al, 2022), still little is known about physiological properties of silk, variables driving phenotypic plasticity, and how behavioral and environmental variables impact silk production (Harrison et al, 2012;Heryanto et al, 2022;Roberts et al, 2020;Tang et al, 2017).…”